1887

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Volume 83, Issue 8

Identification of radically different variants of porcine reproductive and respiratory syndrome virus in Eastern Europe: towards a common ancestor for European and American viruses Free

Abstract

We determined 22 partial porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 sequences, representing pathogenic field strains mainly from Poland and Lithuania, and two currently available European-type live PRRSV vaccines. Also, the complete ORF7 of two Lithuanian and two Polish strains was sequenced. We found that Polish, and in particular Lithuanian, PRRSV sequences were exceptionally different from the European prototype, the Lelystad virus, and in addition showed a very high national diversity. The most diverse present-day European-type PRRSV sequences were from Poland (2000) and Lithuania (2000), and exhibited only 72·2% nucleotide identity in the investigated ORF5 sequence. While all sequences determined in the present study were clearly of European type, inclusion of the new Lithuanian sequences in the genealogy resulted in a common ancestor for the European type virus significantly closer to the American-type PRRSV than previously seen. In addition, the length of the ORF7 of the Lithuanian strains was 378 nucleotides, and thus intermediate between the sizes of the prototypical EU-type (387 nucleotides) and US-type (372 nucleotides) ORF7 lengths. These findings for the Lithuanian PRRSV sequences provide support for the hypothesis that the EU and US genotypes of PRRSV evolved from a common ancestor. Also, this is the first report of ORF7 protein size polymorphism in field isolates of EU-type PRRSV.

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© Microbiology Society
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2002-08-01
2024-04-18
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References

  1. Andreyev V. G., Wesley R. D., Mengeling W. L., Vorwald A. C., Lager K. M. 1997; Genetic variation and phylogenetic relationships of 22 porcine reproductive and respiratory syndrome virus (PRRSV) field strains based on sequence analysis of open reading frame 5. Archives of Virology 142:993–1001
     [Google Scholar]
  2. Andreyev V. G., Scherbakov A. G., Pylnov V. A., Gusev A. A. 1999; Genetic heterogeneity of PRRSV in Russia. In Proceedings of the Third International Symposium on Porcine Reproductive and Respiratory Syndrome (PRRS) pp 211–212
     [Google Scholar]
  3. Balasuriya U. B. R., Patton J. F., Rossitto P. V., Timoney P. J., McCollum W. H., MacLachlan N. J. 1997; Neutralization determinants of laboratory strains and field isolates of equine arteritis virus: identification of four neutralization sites in the amino-terminal ectodomain of the GL envelope glycoprotein. Virology 232:114–128
     [Google Scholar]
  4. Becher P., Orlich M., Kosmidou A., Konig M., Baroth M., Thiel H.-J. 1999; Genetic diversity of pestiviruses: identification of novel groups and implications for classification. Virology 262:64–71
     [Google Scholar]
  5. Bøtner A., Strandbygaard B., Sørensen K. J., Have P., Madsen K. G., Madsen E. S., Alexandersen S. 1997; Appearance of acute PRRS-like symptoms in sow herds after vaccination with a modified live PRRS vaccine. Veterinary Record 141:497–499
     [Google Scholar]
  6. Bøtner A., Nielsen J., Oleksiewicz M. B., Storgaard T. 1999; Heterologous challenge with porcine reproductive and respiratory syndrome (PRRS) vaccine virus: no evidence of reactivation of previous European-type PRRS virus infection. Veterinary Microbiology 68:187–195
     [Google Scholar]
  7. Cavanagh D. 1997; Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Archives of Virology 142:629–633
     [Google Scholar]
  8. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate- phenol-chloroform extraction. Analytical Biochemistry 162:156–159
     [Google Scholar]
  9. Colaço C., Sen S., Thangavelu M., Pinder S., Roser B. 1992; Extraordinary stability of enzymes dried in trehalose: simplified molecular biology. Biotechnology 10:1007–1011
     [Google Scholar]
  10. Dea S., Gagnon C. A., Mardassi H., Pirzadeh B., Rogan D. 2000; Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: comparison of the North American and European isolates. Archives of Virology 145:659–688
     [Google Scholar]
  11. Dewey C., Charbonneau G., Carman S., Hamel A., Nayar G., Friendship R., Eernisse K., Swenson S. 2000; Lelystad-like strain of porcine reproductive and respiratory syndrome virus (PRRSV) identified in Canadian swine. Canadian Veterinary Journal 41:493–494
     [Google Scholar]
  12. Dobbe J. C., van der Meer Y., Spaan W. J., Snijder E. J. 2001; Construction of chimeric arteriviruses reveals that the ectodomain of the major glycoprotein is not the main determinant of equine arteritis virus tropism in cell culture. Virology 288:283–294
     [Google Scholar]
  13. Drew T. W., Lowings J. P., Yapp F. 1997; Variation in open reading frames 3, 4 and 7 among porcine reproductive and respiratory syndrome virus isolates in the UK. Veterinary Microbiology 55:209–221
     [Google Scholar]
  14. Felsenstein J. 1989; PHYLIP – Phylogeny Interference Package (Version 3.2). Cladistics 5:164–166
     [Google Scholar]
  15. Forsberg R., Oleksiewicz M. B., Krabbe Petersen A.-M., Hein J., Bøtner A., Storgaard T. 2001; A molecular clock dates the common ancestor of European-type porcine reproductive and respiratory syndrome virus at more than 10 years before onset of the current epidemic. Virology 289:174–179
     [Google Scholar]
  16. Forsberg R., Storgaard T., Nielsen H. S., Oleksiewicz M. B., Cordioli P., Sala G., Hein J., Bøtner A. 2002; The genetic diversity of European type PRRSV is similar to that of North American type but is geographically skewed within Europe. Virology (in press)
    [Google Scholar]
  17. Gagnon C. A., Dea S. 1998; Differentiation between porcine reproductive and respiratory syndrome virus isolates by restriction fragment length polymorphism of their ORF′s 6 and 7 genes. Canadian Journal of Veterinary Research 62:110–116
     [Google Scholar]
  18. Indik S., Valicek L., Klein D., Klanova J. 2000; Variations in the major envelope glycoprotein GP5 of Czech strains of porcine reproductive and respiratory syndrome virus. Journal of General Virology 81:2497–2502
     [Google Scholar]
  19. Jameson B. A., Wolf H. 1988; The antigenic index: a novel algorithm for predicting antigenic determinants. Computer Applications in the Biosciences 4:181–186
     [Google Scholar]
  20. Kapur V., Elam M. R., Pawlovich T. M., Murtaugh M. P. 1996; Genetic variation in porcine reproductive and respiratory syndrome virus isolates in the Midwestern United States. Journal of General Virology 77:1271–1276
     [Google Scholar]
  21. Keffaber K. K. 1989; Reproductive failure of unknown etiology. American Association of Swine Practitioners Newsletter 1:1–10
     [Google Scholar]
  22. Le Gall A., Legeay O., Bourhy H., Arnauld C., Albina E., Jestin A. 1998; Molecular variation in the nucleoprotein gene (ORF7) of the porcine reproductive and respiratory syndrome virus (PRRSV. Virus Research 54:9–21
     [Google Scholar]
  23. Lindhaus W., Lindhaus B. 1991; Rätselhafte Schweinekrankheit. Praktische Tierarztliche 5:423–425
     [Google Scholar]
  24. Madsen K. G., Hansen C. M., Madsen E. S., Strandbygaard B., Bøtner A., Sørensen K. J. 1998; Sequence analysis of porcine reproductive and respiratory syndrome virus of the American type collected from Danish swine herds. Archives of Virology 143:1683–1700
     [Google Scholar]
  25. Mardassi H., Massie B., Dea S. 1996; Intracellular synthesis, processing, and transport of proteins encoded by ORFs 5 to 7 of porcine reproductive and respiratory syndrome virus. Virology 221:98–112
     [Google Scholar]
  26. Mavromatis I., Kritas S. K., Alexopoulos C., Tsinas A., Kyriakis S. C. 1999; Field evaluation of a live vaccine against porcine reproductive and respiratory syndrome in fattening pigs. Zentralblatt für Veterinaermedizin Reihe B 46:603–612
     [Google Scholar]
  27. Meng X. J. 2000; Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development. Veterinary Microbiology 74:309–329
     [Google Scholar]
  28. Meng X. J., Paul P. S., Halbur P. G. 1994; Molecular cloning and nucleotide sequencing of the 3′-terminal genomic RNA of the porcine reproductive and respiratory syndrome virus. Journal of General Virology 75:1795–1801
     [Google Scholar]
  29. Meng X. J., Paul P. S., Halbur P. G., Lum M. A. 1995; Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): implication for the existence of two genotypes of PRRSV in the U. S. A. and Europe. Archives of Virology 140:745–755
     [Google Scholar]
  30. Meulenberg J. M., Hulst M. M., de Meijer P., Moonen J. M., den Besten A., de Kluyver E. P., Wensvoort G., Moorman R. J. M. 1993; Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192:62–74
     [Google Scholar]
  31. Meulenberg J. J. M., Petersen-den Besten A., De Kluyver E. P., Moormann R. J. M., Schaaper W. M. M., Wensvoort G. 1995; Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus. Virology 206:155–163
     [Google Scholar]
  32. Meulenberg J. J. M., van Nieuwstadt A. P., van EssenZandbergen A., Bosde Ruijter J. N. A., Langeveld J. P. M., Meloen R. H. 1998; Localization and fine mapping of antigenic sites on the nucleocapsid protein N of porcine reproductive and respiratory syndrome virus with monoclonal antibodies. Virology 252:106–114
     [Google Scholar]
  33. Morozov I., Meng X. J., Paul P. S. 1995; Sequence analysis of open reading frames (ORFs) 2 to 4 of a U. S. isolate of porcine reproductive and respiratory syndrome virus. Archives of Virology 140:1313–1319
     [Google Scholar]
  34. Murtaugh M. P., Elam M. R., Kakach L. T. 1995; Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Archives of Virology 140:1451–1460
     [Google Scholar]
  35. Nelsen C. J., Murtaugh M. P., Faaberg K. S. 1999; Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents. Journal of Virology 73:270–280
     [Google Scholar]
  36. Nielsen H., Engelbrecht J., Brunak S., von Heijne G. 1997; Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering 10:1–6
     [Google Scholar]
  37. Nielsen H. S., Storgaard T., Oleksiewicz M. B. 2000; Analysis of ORF 1 in European porcine reproductive and respiratory syndrome virus by long RT–PCR and restriction fragment length polymorphism (RFLP) analysis. Veterinary Microbiology 76:221–228
     [Google Scholar]
  38. Nielsen H. S., Oleksiewicz M. B., Forsberg R., Stadejek T., Bøtner A., Storgaard T. 2001; Reversion of a live porcine reproductive and respiratory syndrome virus vaccine investigated by parallel mutations. Journal of General Virology 82:1263–1272
     [Google Scholar]
  39. Nielsen J., Bøtner A., Bille-Hansen V., Oleksiewicz M. B., Storgaard T. 2002; Experimental infection of late-term pregnant sows with a field isolate of porcine reproductive and respiratory syndrome vaccine-derived virus. Veterinary Microbiology 84:1–13
     [Google Scholar]
  40. Oleksiewicz M. B., Bøtner A., Madsen K. G., Storgaard T. 1998; Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT–PCR amplification of whole viral genes. Veterinary Microbiology 64:7–22
     [Google Scholar]
  41. Oleksiewicz M. B., Bøtner A., Toft P., Grubbe T., Nielsen J., Kamstrup S., Storgaard T. 2000; Emergence of porcine reproductive and respiratory syndrome virus deletion mutants: correlation with the porcine antibody response to a hypervariable site in the ORF3 structural glycoprotein. Virology 267:135–140
     [Google Scholar]
  42. Oleksiewicz M. B., Bøtner A., Toft P., Normann P., Storgaard T. 2001; Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes. Journal of Virology 75:3277–3290
     [Google Scholar]
  43. Oleksiewicz M. B., Normann P., Bøtner A. 2002; Porcine B-cells recognize epitopes which are conserved between the structural proteins of American- and European-type porcine reproductive and respiratory syndrome virus. Journal of General Virology 83:1407–1418
     [Google Scholar]
  44. Pirzadeh B., Gagnon C. A., Dea S. 1998; Genomic and antigenic variations of porcine reproductive and respiratory syndrome virus major envelope GP5 glycoprotein. Canadian Journal of Veterinary Research 62:170–177
     [Google Scholar]
  45. Rodriguez M. J., Sarraseca J., Fominaya J., Cortes E., Sanz A., Casal J. I. 2001; Identification of an immunodominant epitope in the C terminus of glycoprotein 5 of porcine reproductive and respiratory syndrome virus. Journal of General Virology 82:995–999
     [Google Scholar]
  46. Rowland R. R., Steffen M., Ackerman T., Benfield D. A. 1999; The evolution of porcine reproductive and respiratory syndrome virus: quasispecies and emergence of a virus subpopulation during infection of pigs with VR-2332. Virology 259:262–266
     [Google Scholar]
  47. Shibata I., Uruno K., Mori M. 1996; Serological property and replication in cell cultures of porcine reproductive and respiratory syndrome viruses isolated in Japan. Journal of Veterinary Medical Science 58:805–807
     [Google Scholar]
  48. Shimizu M., Yamada S., Murakami Y., Morozumi T., Kobayashi H., Mitani K., Ito N., Kubo M., Kimura K., Kobayashi M. others 1994; Isolation of porcine reproductive and respiratory syndrome (PRRS) virus from Heko-Heko disease of pigs. Journal of Veterinary Medical Science 56:389–391
     [Google Scholar]
  49. Snijder E. J., Meulenberg J. J. M. 1998; The molecular biology of arteriviruses. Journal of General Virology 79:961–979
     [Google Scholar]
  50. Stadejek T., Pejsak Z. 1995; Application of IPMA for serological diagnosis of porcine reproductive and respiratory syndrome (PRRS). Medycyna Weterynaryjna 51:340–342
     [Google Scholar]
  51. Storgaard T., Oleksiewicz M. B., Bøtner A. 1999; Examination of the selective pressures on a live PRRS vaccine virus. Archives of Virology 144:2389–2401
     [Google Scholar]
  52. Strimmer K., von Haeseler A. 1996; Quartet puzzling: a quartet maximum likelihood method for reconstruction tree topologies. Molecular Biology and Evolution 13:964–969
     [Google Scholar]
  53. Suarez P., Zardoya R., Martin M. J., Prieto C., Dopazo J., Solana A., Castro J. M. 1996; Phylogenetic relationships of European strains of porcine reproductive and respiratory syndrome virus (PRRSV) inferred from DNA sequences of putative ORF-5 and ORF-7 genes. Virus Research 42:159–165
     [Google Scholar]
  54. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
     [Google Scholar]
  55. Thorne J. L., Kishino H., Felsenstein J. 1992; Inching toward reality: an improved likelihood model of sequence evolution. Journal of Molecular Evolution 34:3–16
     [Google Scholar]
  56. Verheije M., Kroese M., Rottier P., Meulenberg J. 2001; Viable porcine arteriviruses with deletions proximal to the 3′ end of the genome. Journal of General Virology 82:2607–2614
     [Google Scholar]
  57. Wensvoort G., Terpstra C., Pol J. M. A., ter Laak E. A., Bloemraad M., de Kluyver E. P., Kragten C., van Buiten L., den Besten A., Wagenaar F. others 1991; Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Veterinary Quarterly 13:121–130
     [Google Scholar]
  58. Wootton S. K., Nelson E. A., Yoo D. 1998; Antigenic structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus. Clinical and Diagnostic Laboratory Immunology 5:773–779
     [Google Scholar]
  59. Wu W.-H., Fang Y., Farwell R., Steffen-Bien M., Rowland R. R. R., Christopher-Hennings J., Nelson E. 2001; A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Virology 287:183–191
     [Google Scholar]
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Identification of radically different variants of porcine reproductive and respiratory syndrome virus in Eastern Europe: towards a common ancestor for European and American viruses
J. Gen. Virol. 83, 1861 (2002); https://doi.org/10.1099/0022-1317-83-8-1861
/content/journal/jgv/10.1099/0022-1317-83-8-1861
/content/journal/jgv/10.1099/0022-1317-83-8-1861
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