1887

Abstract

The mature cauliflower mosaic virus (CaMV) capsid protein (CP), if expressed in the absence of other viral proteins, is transported into the plant cell nucleus by the action of a nuclear localization signal (NLS) close to the N terminus. In contrast, virus particles do not enter the nucleus, but dock at the nuclear membrane, a process inhibited by anti-NLS antibodies or by GTPγS, and apparently mediated by interaction of CP with host importin α. The very acidic N-terminal extension of the viral CP precursor inhibits nuclear targeting of the protein and hence the precursor is localized in the cytoplasm. We hypothesize that this provides a control mechanism which ensures that the CP precursor is used for virus assembly in the cytoplasm and that only mature virus particles reach the nuclear pore.

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2002-07-01
2024-04-16
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References

  1. Bastos R., Ribas de Pouplana L., Enarson M., Bodoor K., Burke B. 1997; Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex. Journal of Cell Biology 137:989–1000
    [Google Scholar]
  2. Belgareh N., Doye V. 1997; Dynamics of nuclear pore distribution in nucleoporin mutant yeast cells. Journal of Cell Biology 136:747–759
    [Google Scholar]
  3. Blanc S., Cerutti M., Usmany M., Vlak J. M., Hull R. 1993; Biological activity of cauliflower mosaic virus aphid transmission factor expressed in a heterologous system. Virology 192:643–650
    [Google Scholar]
  4. Bucci M., Wente S. R. 1997; In vivo dynamics of nuclear pore complexes in yeast. Journal of Cell Biology 136:1185–1199
    [Google Scholar]
  5. Cheng R. H., Olson N. H., Baker T. S. 1992; Cauliflower mosaic virus: a 420 subunit (T=7), multilayer structure. Virology 186:655–668
    [Google Scholar]
  6. Dickmanns A., Bischoff F. R., Marshallsay C., Luhrmann R., Ponstingl H., Fanning E. 1996; The thermolability of nuclear protein import in tsBN2 cells is suppressed by microinjected Ran-GTP or Ran-GDP, but not by RanQ69L or RanT24N. Journal of Cell Science 109:1449–1457
    [Google Scholar]
  7. Du Plessis D. H., Smith P. 1981; Glycosylation of the cauliflower mosaic virus capsid polypeptide. Virology 109:403–408
    [Google Scholar]
  8. Feldherr C. M., Kallenbach E., Schultz N. 1984; Movement of a karyophilic protein through the nuclear pores of oocytes. Journal of Cell Biology 99:2216–2222
    [Google Scholar]
  9. Freed E. O., Englund G., Maldarelli F., Martin M. A. 1997; Phosphorylation of residue 131 of HIV-1 matrix is not required for macrophage infection. Cell 88:171–173
    [Google Scholar]
  10. Fütterer J., Gordon K., Pfeiffer P., Hohn T. 1988; The instability of a recombinant plasmid, caused by a prokaryotic-like promoter within the eukaryotic insert, can be alleviated by expression of antisense RNA. Gene 67:141–145
    [Google Scholar]
  11. Fütterer J., Gordon K., Pfeiffer P., Sanfaçon H., Pisan B., Bonneville J. M., Hohn T. 1989; Differential inhibition of downstream gene expression by the CaMV 35S RNA leader. Virus Genes 3:45–55
    [Google Scholar]
  12. Fütterer J., Bonneville J. M., Gordon K., DeTapia M., Karlsson S., Hohn T. 1990; Expression from polycistronic cauliflower mosaic virus pregenomic RNA. In Posttranscriptional Control of Gene Expression pp 349–357 Edited by McCarthy J. E. G., Tuite M. F. Berlin: Springer;
    [Google Scholar]
  13. Gallay P., Swingler S., Song J., Bushman F., Trono D. 1995; HIV nuclear import is governed by the phosphotyrosine-mediated binding of matrix to the core domain of integrase. Cell 83:569–576
    [Google Scholar]
  14. Gallay P., Hope T., Chin D., Trono D. 1997; HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway. Proceedings of the National Academy of Sciences, USA 94:9825–9830
    [Google Scholar]
  15. Gelderblom H. R., Özel M., Pauli G. 1989; Morphogenesis and morphology of HIV structure-function relations. Archives of Virology 106:1–13
    [Google Scholar]
  16. Goodall G. J., Wiebauer K., Filipowicz W. 1990; Analysis of pre-mRNA processing in transfected plant protoplasts. Methods in Enzymology 181:148–161
    [Google Scholar]
  17. Guerra-Peraza O., de Tapia M., Hohn T., Hemmings-Mieszczak M. 2000; Interaction of the cauliflower mosaic virus coat protein with the pregenomic RNA leader. Journal of Virology 74:2067–2072
    [Google Scholar]
  18. Himmelbach A., Chapdelaine Y., Hohn T. 1996; Interaction between cauliflower mosaic virus inclusion body protein and capsid protein: implications for viral assembly. Virology 217:147–157
    [Google Scholar]
  19. Hurkman W. J., Tanaka C. K. 1986; Solubilization of membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiology 81:802–806
    [Google Scholar]
  20. Izaurralde E., Kann M., Panté N., Sodeik B., Hohn T. 1999; Viruses, microorganisms and scientists meet the nuclear pore. EMBO Journal 18:289–296
    [Google Scholar]
  21. Kann M., Bischof A., Gerlich W. H. 1997; In vitro model for the nuclear transport of the hepadnavirus genome. Journal of Virology 71:1310–1316
    [Google Scholar]
  22. Kann M., Sodeik B., Vlachou A., Gerlich W. H., Helenius A. 1999; Phosphorylation-dependent binding of hepatitis B virus core particles to the nuclear pore complex. Journal of Cell Biology 145:45–55
    [Google Scholar]
  23. Karsies A., Hohn T., Leclerc D. 2001; Degradation signals within both terminal domains of the cauliflower mosaic virus capsid protein precursor. Plant Journal 27:335–343
    [Google Scholar]
  24. Kunik T., Palanichelvam K., Czosnek H., Citovsky V., Gafni Y. 1998; Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect cells. Plant Journal 13:393–399
    [Google Scholar]
  25. Lebeurier G., Hirth L., Hohn T., Hohn B. 1980; Infectivities of native and cloned DNA of cauliflower mosaic virus. Gene 12:139–146
    [Google Scholar]
  26. Leclerc D., Chapdelaine Y., Hohn T. 1999; Nuclear targeting of the cauliflower mosaic virus coat protein. Journal of Virology 73:553–560
    [Google Scholar]
  27. Martinez-Izquierdo J., Hohn T. 1987; Cauliflower mosaic virus coat protein is phosphorylated in vitro by a virion associated protein kinase. Proceedings of the National Academy of Sciences, USA 84:1824–1828
    [Google Scholar]
  28. Merkle T., Leclerc D., Marshallsay C., Nagy F. 1996; A plant in vitro system for the nuclear import of proteins. Plant Journal 10:1177–1186
    [Google Scholar]
  29. Mieszczak M., Klahre U., Levy J. H., Goodall G. J., Filipowicz W. 1992; Multiple plant RNA binding proteins identified by PCR: expression of cDNAs encoding RNA binding proteins targeted to chloroplasts in Nicotiana plumbaginifolia. Molecular and General Genetics 234:390–400
    [Google Scholar]
  30. Moroianu J., Blobel G., Radu A. 1996; Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta. Proceedings of the National Academy of Sciences, USA 93:7059–7062
    [Google Scholar]
  31. Panté N., Aebi U. 1996; Sequential binding of import ligands to distinct nucleopore regions during their nuclear import. Science 273:1729–1732
    [Google Scholar]
  32. Panté N., Kann M. 2002; The nuclear pore complex is able to transport macromolecules with diameters of about 39 nm. Molecular Biology of the Cell 10·1091/mbc.01-06-0308
    [Google Scholar]
  33. Roe T.-Y., Reynolds T. C., Yu G., Brown P. O. 1993; Integration of murine leukemia virus DNA depends on mitosis. EMBO Journal 12:2099–2108
    [Google Scholar]
  34. Rothnie H. M., Chapdelaine Y., Hohn T. 1994; Pararetroviruses and retroviruses: a comparative review of viral structure and gene expression strategies. Advanced Virus Research 44:1–67
    [Google Scholar]
  35. Schagger H., von Jagow G. 1987; Tricine–sodium dodecyl sulfate–polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry 166:368–379
    [Google Scholar]
  36. Smith H. M., Hicks G. R., Raikhel N. V. 1997; Importin alpha from Arabidopsis thaliana is a nuclear import receptor that recognizes three classes of import signals. Plant Physiology 114:411–417
    [Google Scholar]
  37. Szurek B., Marois E., Bonas U., Van den Ackerveken G. 2001; Eukaryotic features of the Xanthomonas type III effector AvrBs3: protein domains involved in transcriptional activation and the interaction with nuclear import receptors from pepper. Plant Journal 26:1–16
    [Google Scholar]
  38. Torruella M., Gordon K., Hohn T. 1989; Cauliflower mosaic virus produces an aspartic proteinase to cleave its polyproteins. EMBO Journal 8:2819–2825
    [Google Scholar]
  39. Whittaker G. R., Helenius A. 1998; Nuclear import and export of viruses and virus genomes. Virology 246:1–23
    [Google Scholar]
  40. Yamada M., Kasamatsu H. 1993; Role of nuclear pore complexes in SV40 nuclear targeting. Journal of Virology 67:119–130
    [Google Scholar]
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