1887

Abstract

The 2b protein of (PEBV) is required for transmission of the virus by nematodes. Comparison of the 2b proteins of highly transmissible (TpA56) and poorly transmissible (SP5) isolates of PEBV identified two amino acid substitutions (G90S and G177R) that might be responsible for the poor transmission of isolate SP5. Hybrid viruses were created in which the TpA56 2b protein carried SP5-specific substitutions at residue 90 or 177, and in which the SP5 2b protein carried TpA56-specific substitutions at these positions. Transmission tests showed that the G177R substitution is sufficient to prevent nematode transmission of the virus. Examination of the 2b proteins from PEBV and other tobraviruses predicted the presence of a coiled-coil domain in the central region of the protein. This structural element is important for the association of interacting proteins and, thus, might mediate interaction of the 2b protein with the virus coat protein or with the vector nematode.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-7-1771
2002-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/7/0831771a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-7-1771&mimeType=html&fmt=ahah

References

  1. Harrison B. D., Robinson D. J. 1986; Tobraviruses. In The Plant Viruses pp 339–369 Edited by van Regenmortel M. H. V., Fraenkel-Conrat H. New York: Plenum Press;
    [Google Scholar]
  2. Hernández C., Carette J. E., Brown D. J. F., Bol J. F. 1996; Serial passage of tobacco rattle virus under different selection conditions results in deletion of structural and non-structural genes in RNA2. Journal of Virology 70:4933–4940
    [Google Scholar]
  3. Hernández C., Visser P. B., Brown D. J. F., Bol J. F. 1997; Transmission of tobacco rattle virus isolate PpK20 by its nematode vector requires one of the two non-structural genes in the viral RNA2. Journal of General Virology 78:465–467
    [Google Scholar]
  4. Karanastasi E. 2001; Acquisition, retention, and transmission of tobravirus particles by nematodes of the family Trichodoridae, and intervention of the trichodorid feeding behaviour and pharyngeal ultrastructure. PhD thesis University of Dundee; UK:
  5. Karanastasi E., Vassilakos N., Roberts I. M., MacFarlane S. A., Brown D. J. F. 2000; Immunogold localisation of tobacco rattle virus particles within the virus-vector nematode Paratrichodorus anemones. Journal of Nematology 32:5–12
    [Google Scholar]
  6. Leclerc D., Burri L., Kajava A. V., Mougeot J.-L., Hess D., Lustig A., Kleeman G., Hohn T. 1998; The open reading frame III product of cauliflower mosaic virus forms a tetramer though a N-terminal coiled-coil. Journal of Biological Chemistry 273:29015–29021
    [Google Scholar]
  7. Leh V., Jacquot E., Geldreich A., Hermann T., Leclerc D., Cerutti M., Yot P., Keller M., Blanc S. 1999; Aphid transmission of cauliflower mosaic virus requires the viral PIII protein. EMBO Journal 18:7077–7085
    [Google Scholar]
  8. Lupas A. 1996; Coiled coils: new structures and new functions. Trends in Biochemical Sciences 21:375–382
    [Google Scholar]
  9. MacFarlane S. A. 1999; The molecular biology of the tobraviruses. Journal of General Virology 80:2799–2807
    [Google Scholar]
  10. MacFarlane S. A., Brown D. J. F. 1995; Sequence comparison of RNA2 of nematode-transmissible and nematode-non-transmissible isolates of pea early-browning virus suggests that the gene encoding the 29 kDa protein may be involved in nematode transmission. Journal of General Virology 76:1299–1304
    [Google Scholar]
  11. MacFarlane S. A., Wallis C. V., Taylor S. C., Goulden M. G., Wood K. R., Davies J. W. 1991; Construction and analysis of infectious transcripts synthesized from full-length cDNA clones of both genomic RNAs of pea early-browning virus. Virology 182:124–129
    [Google Scholar]
  12. MacFarlane S. A., Wallis C. V., Brown D. J. F. 1996; Multiple genes involved in the nematode transmission of pea early-browning virus. Virology 219:417–422
    [Google Scholar]
  13. Ploeg A. T., Robinson D. J., Brown D. J. F. 1993; RNA2 of tobacco rattle virus encodes the determinants of transmissibility by trichodorid vector nematodes. Journal of General Virology 74:1463–1466
    [Google Scholar]
  14. Taylor C. E., Brown D. J. F. 1997 Nematode Vectors of Plant Viruses New York: CAB International;
    [Google Scholar]
  15. Thompson J. T., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
    [Google Scholar]
  16. Vassilakos N. 2000; Molecular studies of the specificity of nematode transmission of Tobacco rattle virus. PhD thesis University of Dundee; UK:
  17. Vassilakos N., Vellios E. K., Brown E. C., Brown D. J. F., MacFarlane S. A. 2001; Tobravirus 2b protein acts in trans to facilitate transmission by nematodes. Virology 279:478–487
    [Google Scholar]
  18. Verwoerd T. C., Dekker B. M. M., Hoekema A. 1989; A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Research 17:2362
    [Google Scholar]
  19. Williams G. W., Faller M. 1999; PIX: a peptide identification system at the HGMP-RC. http://www.hgmp.mrc.ac.uk/Registered/Webapp/pix
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-7-1771
Loading
/content/journal/jgv/10.1099/0022-1317-83-7-1771
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error