1887

Abstract

Monoclonal antibodies were raised against helper component-proteinase (HcPro) purified from plants infected with the potyvirus (LMV). These antibodies were used in a two-site triple antibody sandwich ELISA assay together with polyclonal antibodies directed against purified virions. An interaction between HcPro and the viral coat protein (CP) was demonstrated in extracts of LMV-infected leaves, as well as for two other potyviruses, and . The CP–HcPro interaction was not abolished in LMV derivatives with an HcPro GFP N-terminal fusion, or with a deletion from the CP of the amino acids involved in aphid transmission. Electron microscopy indicated that HcPro probably does not interact with the CP in the form of assembled virions or virus-like particles. Together, these results suggest that the interaction detected between CP and HcPro might be involved in a process of the potyvirus cycle different from aphid transmission.

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2002-07-01
2019-10-15
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References

  1. Anandalakshmi, R., Pruss, G. J., Ge, X., Marathe, R., Mallory, A. C., Smith, T. H. & Vance, V. B. ( 1998; ). A viral suppressor of gene silencing in plants. Proceedings of the National Academy of Sciences, USA 95, 13079-13084.[CrossRef]
    [Google Scholar]
  2. Andrejeva, J., Puurand, U., Merits, A., Rabenstein, F., Jarvekulg, L. & Valkonen, J. P. ( 1999; ). Potyvirus helper component-proteinase and coat protein (CP) have coordinated functions in virus–host interactions and the same CP motif affects virus transmission and accumulation. Journal of General Virology 80, 1133-1139.
    [Google Scholar]
  3. Atreya, P. L., Atreya, C. D. & Pirone, T. P. ( 1991; ). Amino acid substitutions in the coat protein result in loss of insect transmissibility of a plant virus. Proceedings of the National Academy of Sciences, USA 88, 7887-7897.[CrossRef]
    [Google Scholar]
  4. Blanc, S., Lopez-Moya, J. J., Wang, R., Garcia-Lampasona, S., Thornbury, D. W. & Pirone, T. P. ( 1997; ). A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus. Virology 231, 141-147.[CrossRef]
    [Google Scholar]
  5. Brigneti, G., Voinnet, O., Li, W. X., Ji, L. H., Ding, S. W. & Baulcombe, D. C. ( 1998; ). Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO Journal 17, 6739-6746.[CrossRef]
    [Google Scholar]
  6. Carrington, J. C., Cary, S. M., Parks, T. D. & Dougherty, W. G. ( 1989; ). A second proteinase encoded by a plant potyvirus genome. EMBO Journal 8, 365-370.
    [Google Scholar]
  7. Clark, M. F. & Adams, A. N. ( 1977; ). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34, 475-483.[CrossRef]
    [Google Scholar]
  8. Fakhfakh, H., Le Gall, O., Candresse, T. & Marrakchi, M. ( 2001; ). Analysis of the variability of Tunisian isolates of Lettuce mosaic potyvirus (LMV) using biological and molecular properties. Journal of Plant Pathology 83, 3-11.
    [Google Scholar]
  9. German-Retana, S., Candresse, T., Alias, E., Delbos, R. & Le Gall, O. ( 2000; ). Effects of GFP or GUS tagging on the accumulation and pathogenicity of a resistance breaking LMV isolate in susceptible and resistant lettuce cultivars. Molecular Plant–Microbe Interactions 13, 316-324.[CrossRef]
    [Google Scholar]
  10. Guo, D., Rajamaki, M. L., Saarma, M. & Valkonen, J. P. ( 2001; ). Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system. Journal of General Virology 82, 935-939.
    [Google Scholar]
  11. Huet, H., Gal-On, A., Meir, E., Lecoq, H. & Raccah, B. ( 1994; ). Mutations in the helper component protease gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility. Journal of General Virology 75, 1407-1414.[CrossRef]
    [Google Scholar]
  12. Kasschau, K. D. & Carrington, J. C. ( 1998; ). A counter-defensive strategy of plant viruses: suppression of posttranscriptional gene silencing. Cell 95, 461-470.[CrossRef]
    [Google Scholar]
  13. Kasschau, K. D. & Carrington, J. C. ( 2001; ). Long-distance movement and replication maintenance functions correlate with silencing suppression activity of potyviral HC-Pro. Virology 285, 71-81.[CrossRef]
    [Google Scholar]
  14. Kasschau, K. D., Cronin, S. & Carrington, J. C. ( 1997; ). Genome amplification and long-distance movement functions associated with the central domain of tobacco etch potyvirus helper component-proteinase. Virology 228, 251-262.[CrossRef]
    [Google Scholar]
  15. Kerlan, C. & Dunez, J. ( 1979; ). Différenciation biologique et sérologique de souches du virus de la Sharka. Annals of Phytopathology 11, 241-251.
    [Google Scholar]
  16. Klein, P. G., Klein, R. R., Rodriguez-Cerezo, E., Hunt, A. G. & Shaw, J. G. ( 1994; ). Mutational analysis of the tobacco vein mottling virus genome. Virology 204, 759-769.[CrossRef]
    [Google Scholar]
  17. Krause-Sakate, R., Le Gall, O., Fakhfakh, H., Peypelut, M., Marrakchi, M., Varveri, C., Pavan, M. A., Souche, S., Lot, H., Zerbini, F. M. & Candresse, T. (2002). Molecular characterization of Lettuce mosaic virus field isolates reveals the emergence and spread of a resistance-breaking strain, LMV-Most. Phytopathology.
  18. Maia, I. G., Haenni, A.-L. & Bernardi, F. ( 1996; ). Potyviral HC-Pro: a multifunctional protein. Journal of General Virology 77, 1335-1341.[CrossRef]
    [Google Scholar]
  19. Maiss, E., Timpe, U., Brisske, A., Jelkmann, W., Casper, R., Himmler, G., Mattanovich, D. & Katinger, H. W. D. ( 1989; ). The nucleotide sequence of plum pox virus RNA. Journal of General Virology 70, 513-524.[CrossRef]
    [Google Scholar]
  20. Manoussopoulos, I. N., Maiss, E. & Tsagris, M. ( 2000; ). Native electrophoresis and Western blot analysis (NEWeB): a method for characterization of different forms of potyvirus particles and similar nucleoprotein complexes in extracts of infected plant tissues. Journal of General Virology 81, 2295-2298.
    [Google Scholar]
  21. Peng, Y., Kadoury, D., Gal-On, A., Huet, H., Wang, Y. & Raccah, B. ( 1998; ). Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions. Journal of General Virology 79, 897-904.
    [Google Scholar]
  22. Redondo, E., Krause-Sakate, R., Yang, S. J., Lot, H., Le Gall, O. & Candresse, T. ( 2001; ). Lettuce mosaic virus (LMV) pathogenicity determinants in susceptible and tolerant lettuce varieties map to different regions of the viral genome. Molecular Plant–Microbe Interactions 14, 804-810.[CrossRef]
    [Google Scholar]
  23. Revers, F., Yang, S. J., Walter, J., Souche, S., Lot, H., Le Gall, O., Candresse, T. & Dunez, J. ( 1997; ). Comparison of the complete nucleotide sequences of two isolates of Lettuce mosaic virus differing in their biological properties. Virus Research 47, 167-177.[CrossRef]
    [Google Scholar]
  24. Revers, F., Le Gall, O., Candresse, T. & Maule, A. J. ( 1999; ). New advances in understanding the molecular biology of plant/potyvirus interactions. Molecular Plant–Microbe Interactions 12, 367-376.[CrossRef]
    [Google Scholar]
  25. Rojas, M. R., Zerbini, F. M., Allison, R. F., Gilbertson, R. L. & Lucas, W. J. ( 1997; ). Capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins. Virology 237, 283-295.[CrossRef]
    [Google Scholar]
  26. Shukla, D. D., Ward, C. W. & Brunt, A. A. (1994). The Potyviridae. Wallingford, UK: CAB International.
  27. Vance, V. B., Berger, P. H., Carrington, J. C., Hunt, A. G. & Shi, X. M. ( 1995; ). 5′ proximal potyviral sequences mediate potato virus X/potyviral synergistic disease in transgenic tobacco. Virology 206, 583-590.[CrossRef]
    [Google Scholar]
  28. Yang, S. J., Revers, F., Souche, S., Lot, H., Le Gall, O., Candresse, T. & Dunez, J. ( 1998; ). Construction of full-length cDNA clones of Lettuce mosaic virus (LMV) and the effects of intron-insertion on their viability in Escherichia coli and on their infectivity to plants. Archives of Virology 143, 2443-2451.[CrossRef]
    [Google Scholar]
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