1887

Abstract

Jaagsiekte sheep retrovirus (JSRV) is the aetiological agent of ovine pulmonary adenocarcinoma (OPA). To monitor changes in cellular immune function during JSRV infection, lymphoproliferation in response to various mitogens was measured in the blood of conventionally housed and specific-pathogen-free lambs experimentally infected with JSRV until the development of OPA and compared with uninfected control lambs. In addition, blood samples collected from adult field cases in the terminal stages of OPA and control adult sheep were compared. No difference in the proliferative response to phytohaemagglutinin and pokeweed mitogen between the animal groups was detected. In contrast, reduced responses to concanavalin A stimulation were demonstrated in the JSRV-inoculated lambs, prior to the onset of clinical disease, and also in the terminally ill adult sheep. Peripheral blood leukocytes were monitored to identify phenotypic frequency alterations. The CD4 lymphocytopaenia and neutrophilia reported previously in adult OPA cases were demonstrated but similar phenotypic changes were not identified during experimental infection.

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2002-07-01
2019-10-14
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References

  1. Bachh, A. S., Ram, G. C., Hopkins, J. & Bansal, M. P. ( 1995; ). Phenotypic characterisation of ovine lymphocyte subpopulations. Immunoadherence and reactivity to monoclonal antibodies. Indian Veterinary Journal 72, 680-686.
    [Google Scholar]
  2. Boyum, A. ( 1968; ). Separation of leukocytes from blood and bone marrow. Scandinavian Journal of Clinical and Laboratory Investigation 21 (Suppl. 97), S7.
    [Google Scholar]
  3. DeMartini, J. C., Bishop, J. V., Allen, T. E., Jassim, F. A., Sharp, J. M., de las Heras, M., Voelker, D. R. & Carlson, J. O. ( 2001; ). Jaagsiekte sheep retrovirus proviral clone JSRVJS7, derived from the JS7 lung tumor cell line, induces ovine pulmonary carcinoma and is integrated into the surfactant protein A gene. Journal of Virology 75, 4239-4246.[CrossRef]
    [Google Scholar]
  4. Dunlap, J. E., Nichols, W. S., Hebebrand, L. C., Mathes, L. E. & Olsen, R. G. ( 1979; ). Mobility of lymphocyte surface membrane concanavalin A receptors of normal and feline leukaemia virus-infected viremic felines. Cancer Research 39, 956-958.
    [Google Scholar]
  5. Holland, M. J., Palmarini, M., Garcia-Goti, M., Gonzalez, L., McKendrick, I., de las Heras, M. & Sharp, J. M. ( 1999; ). Jaagsiekte retrovirus is widely distributed both in T and B lymphocytes and in mononuclear phagocytes of sheep with naturally and experimentally acquired pulmonary adenomatosis. Journal of Virology 73, 4004-4008.
    [Google Scholar]
  6. Kilpatrick, D. C. (1998). In Lectin Methods and Protocols, pp. 385–392. Edited by J. M. Rhodes & J. D. Milton. Totowa, NJ: Humana Press.
  7. Lloyd, J. B., Gill, H. S. & Husband, A. J. ( 1995; ). The effect of storage on immunophenotyping of sheep peripheral blood lymphocytes by flow cytometry. Veterinary Immunology and Immunopathology 47, 135-142.[CrossRef]
    [Google Scholar]
  8. Maeda, N., Palmarini, M., Murgia, C. & Fan, H. ( 2001; ). Direct transformation of rodent fibroblasts by Jaagsiekte sheep retrovirus DNA. Proceedings of the National Academy of Sciences, USA 98, 4449-4454.[CrossRef]
    [Google Scholar]
  9. Mann, D. L., Lasane, F., Popovic, M., Arthur, L. O., Robey, W. G., Blattner, W. A. & Newman, M. J. ( 1987; ). HTLV-III large envelope protein (gp120) suppresses PHA-induced lymphocyte blastogenesis. Journal of Immunology 138, 2640-2644.
    [Google Scholar]
  10. Martin, W. B., Scott, F. M., Sharp, J. M., Angus, K. W. & Norval, M. ( 1976; ). Experimental production of sheep pulmonary adenomatosis (Jaagsiekte). Nature 264, 183-185.[CrossRef]
    [Google Scholar]
  11. Mathes, L. E., Olsen, R. G., Hebebrand, L. C., Hoover, E. A. & Schaller, J. P. ( 1978; ). Abrogation of lymphocyte blastogenesis by a feline leukaemia virus protein. Nature 274, 687-689.[CrossRef]
    [Google Scholar]
  12. Mathes, L. E., Olsen, R. G., Hebebrand, L. C., Hoover, E. A., Schaller, J. P., Adams, P. W. & Nichols, W. S. ( 1979; ). Immunosuppressive properties of a virion polypeptide, a 15,000-dalton protein from feline leukemia virus. Cancer Research 39, 950-955.
    [Google Scholar]
  13. Miedema, F., Petit, A. J., Terpstra, F. G., Schattenkerk, J. K., De Wolf, F., Al, B. J., Roos, M., Lange, J. M., Danner, S. A., Goudsmit, J. and others ( 1988; ). Immunological abnormalities in human immunodeficiency virus (HIV)-infected asymptomatic homosexual men. HIV affects the immune system before CD4+ T helper cell depletion occurs. Journal of Clinical Investigation 82, 1908–1914.[CrossRef]
    [Google Scholar]
  14. Ortin, A., Minguijon, E., Dewar, P., Garcia, M., Ferrer, L. M., Palmarini, M., Gonzalez, L., Sharp, J. M. & de las Heras, M. ( 1998; ). Lack of a specific immune response against a recombinant capsid protein of Jaagsiekte sheep retrovirus in sheep and goats naturally affected by enzootic nasal tumour or sheep pulmonary adenomatosis. Veterinary Immunology and Immunopathology 61, 229-237.[CrossRef]
    [Google Scholar]
  15. Palmarini, M., Dewar, P., de las Heras, M., Inglis, N. F., Dalziel, R. G. & Sharp, J. M. ( 1995; ). Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus. Journal of General Virology 76, 2731-2737.[CrossRef]
    [Google Scholar]
  16. Palmarini, M., Cousens, C., Dalziel, R. G., Bai, J., Stedman, K., DeMartini, J. C. & Sharp, J. M. ( 1996a; ). The exogenous form of Jaagsiekte retrovirus is specifically associated with a contagious lung cancer of sheep. Journal of Virology 70, 1618-1623.
    [Google Scholar]
  17. Palmarini, M., Holland, M. J., Cousens, C., Dalziel, R. G. & Sharp, J. M. ( 1996b; ). Jaagsiekte retrovirus establishes a disseminated infection of the lymphoid tissues of sheep affected by pulmonary adenomatosis. Journal of General Virology 77, 2991-2998.[CrossRef]
    [Google Scholar]
  18. Palmarini, M., Sharp, J. M., de las Heras, M. & Fan, H. ( 1999; ). Jaagsiekte sheep retrovirus is necessary and sufficient to induce a contagious lung cancer of sheep. Journal of Virology 73, 6964-6972.
    [Google Scholar]
  19. Palmarini, M., Maeda, N., Murgia, C., De-Fraja, C., Hofacre, A. & Fan, H. ( 2001; ). A phosphatidylinositol 3-kinase docking site in the cytoplasmic tail of the Jaagsiekte sheep retrovirus transmembrane protein is essential for envelope-induced transformation of NIH 3T3 cells. Journal of Virology 75, 11002-11009.[CrossRef]
    [Google Scholar]
  20. Rai, S. K., Duh, F. M., Vigdorovich, V., Danilkovitch-Miagkova, A., Lerman, M. I. & Miller, A. D. ( 2001; ). Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GP1)-anchored cell-surface receptor for Jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation. Proceedings of the National Academy of Sciences, USA 98, 4443-4448.[CrossRef]
    [Google Scholar]
  21. Roche, S., Koegl, M. & Courtneidge, S. A. ( 1994; ). The phosphatidylinositol 3-kinase α is required for DNA synthesis induced by some, but not all, growth factors. Proceedings of the National Academy of Sciences, USA 91, 9185-9189.[CrossRef]
    [Google Scholar]
  22. Rosadio, R. H. & Sharp, J. M. (1992). Leukocyte frequency alterations in sheep with naturally and experimentally induced lung cancer. Veterinary Medicine, 49–51.
  23. Ruegg, C. L., Monell, C. R. & Strand, M. ( 1989; ). Identification, using synthetic peptides, of the minimum amino acid sequence from the retroviral transmembrane protein p15E required for inhibition of lymphoproliferation and its similarity to gp21 of human T-lymphotropic virus types I and II. Journal of Virology 63, 3250-3256.
    [Google Scholar]
  24. Schmidt, D. M., Sidhu, N. K., Cianciolo, G. J. & Snyderman, R. ( 1987; ). Recombinant hydrophilic region of murine retroviral protein p15E inhibits stimulated T-lymphocyte proliferation. Proceedings of the National Academy of Sciences, USA 84, 7290-7294.[CrossRef]
    [Google Scholar]
  25. Sharp, J. M. & Herring, A. J. ( 1983; ). Sheep pulmonary adenomatosis: demonstration of a protein which cross-reacts with the major core proteins of Mason–Pfizer monkey virus and mouse mammary tumour virus. Journal of General Virology 64, 2323-2327.[CrossRef]
    [Google Scholar]
  26. Sharp, J. M. & Angus, K. W. ( 1990; ). Sheep pulmonary adenomatosis: clinical, pathological and epidemiological aspects. In Developments in Veterinary Virology: Maedi–Visna and Related diseases , pp. 157-175. Edited by G. Petursson & R. H. Jorgensen. Boston:Kluwer.
  27. Sharp, J. M., Angus, K. W., Gray, E. W. & Scott, F. M. ( 1983; ). Rapid transmission of sheep pulmonary adenomatosis (Jaagsiekte) in young lambs. Archives of Virology 78, 89-95.[CrossRef]
    [Google Scholar]
  28. York, D. F., Vigne, R., Verwoerd, D. W. & Querat, G. ( 1991; ). Isolation, identification, and partial cDNA cloning of genomic RNA of Jaagsiekte retrovirus, the etiological agent of sheep pulmonary adenomatosis. Journal of Virology 65, 5061-5067.
    [Google Scholar]
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