1887

Abstract

It was reported previously that haemadsorption by the haemagglutinin (HA) protein of influenza B virus required that the protein must undergo desialidation. When MDCK and COS cells were infected with influenza B/Kanagawa/73 virus in the presence of a neuraminidase (NA) inhibitor, Zanamivir, haemadsorption on MDCK cells was inhibited but that on COS cells was not. The activity of the NA protein of the two types of infected cells was similar and both were inhibited by Zanamivir in a dose-dependent manner. A comparison of the desialidation of the HA protein was made on MDCK and COS cells in the presence of bacterial NA and both cells were found to have similar sensitivity. On the accumulation of the HA and NA proteins in the trans-Golgi network of MDCK cells by means of low-temperature treatment, desialidation of the HA protein in the presence of Zanamivir was detected by two-dimensional gel electrophoresis. Because this agent was reported to be unable to penetrate cells, these data suggest that, in MDCK cells, desialidation of the HA protein occurs on the cell surface but, in COS cells, the HA and NA proteins might accumulate in the trans-Golgi network, thus allowing NA desialidation before their migration to the cell surface.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-7-1729
2002-07-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/7/0831729a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-7-1729&mimeType=html&fmt=ahah

References

  1. Brassard, D. & Lamb, R. A. ( 1997; ). Expression of influenza B virus hemagglutinin containing multibasic residue cleavage sites. Virology 236, 234-248.[CrossRef]
    [Google Scholar]
  2. Enami, M. & Enami, K. ( 1996; ). Influenza virus hemagglutinin and neuraminidase glycoproteins stimulate the membrane association of the matrix protein. Journal of Virology 70, 6653-6657.
    [Google Scholar]
  3. Griffiths, G., Pfeiffer, S., Simons, K. & Matlin, K. ( 1985; ). Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane. Journal of Cell Biology 101, 949-964.[CrossRef]
    [Google Scholar]
  4. Itzstein, M. V., Wu, W.-Y., Kok, G. B., Pegg, M. S., Dyason, J. C., Jin, B., Phan, T. V., Smythe, M. L., White, H. F., Oliver, S. W., Colman, P. M., Vargheses, J. N., Ryan, D. M., Woods, J. M., Bethell, R. C., Hotham, V. J., Cameron, J. M. & Penn, C. R. ( 1993; ). Rational design of potent sialidase-based inhibitors of influenza virus replication. Nature 363, 418-423.[CrossRef]
    [Google Scholar]
  5. Luo, C., Nobusawa, E. & Nakajima, K. ( 1999; ). An analysis of the role of neuraminidase in the receptor-binding activity of influenza B virus: the inhibitory effect of Zanamivir on haemadsorption. Journal of General Virology 80, 2969-2976.
    [Google Scholar]
  6. Morishita, T., Nobusawa, E., Nakajima, K. & Nakajima, S. ( 1996; ). Studies on the molecular basis for loss of the ability of recent influenza A (H1N1) virus strains to agglutinate chicken erythrocytes. Journal of General Virology 77, 2499-2506.[CrossRef]
    [Google Scholar]
  7. Morris, S. J., Price, G. E., Barnett, J. M., Hiscox, S. A., Smith, H. & Sweet, C. ( 1999; ). Role of neuraminidase in influenza virus-induced apoptosis. Journal of General Virology 80, 137-146.
    [Google Scholar]
  8. Nobusawa, E. & Nakajima, K. ( 1988; ). Amino acid substitution at position 226 of the hemagglutinin molecule of influenza (H1N1) virus affects receptor binding activity but not fusion activity. Virology 167, 8-14.[CrossRef]
    [Google Scholar]
  9. Ohuchi, M., Feldmann, A., Ohuchi, R. & Klenk, H.-D. ( 1995; ). Neuraminidase is essential for fowl plague virus hemagglutinin to show hemagglutinating activity. Virology 212, 77-83.[CrossRef]
    [Google Scholar]
  10. Palese, P., Tobita, K., Ueda, M. & Compans, R. W. ( 1974; ). Characterization of temperature sensitive influenza virus mutants defective in neuraminidase. Virology 61, 397-410.[CrossRef]
    [Google Scholar]
  11. Palmer, D. F., Coleman, M. T., Dowdle, W. R. & Schild, G. C. (1975). Neuraminidase inhibition test. In Advanced Laboratory Techniques for Influenza Diagnosis, pp. 107–126. Center for Disease Control, Atlanta, USA.
  12. Shibata, S., Yamamoto-Goshima, F., Maeno, K., Hanaichi, T., Fujita, Y., Nakajima, K., Imai, M., Komatsu, T. & Sugiura, S. ( 1993; ). Characterization of a temperature-sensitive influenza B virus mutant defective in neuraminidase. Journal of Virology 67, 3264-3273.
    [Google Scholar]
  13. Tong, N., Nobusawa, E., Morishita, M., Nakajima, S. & Nakajima, K. ( 1998; ). M protein correlates with the receptor-binding specificity of haemagglutinin protein of reassortant influenza A (H1N1) virus. Journal of General Virology 79, 2425-2434.
    [Google Scholar]
  14. Wandinger-Ness, A., Bennett, M. K., Antony, C. & Simons, K. ( 1990; ). Distinct transport vesicles mediate the delivery of plasma membrane proteins to the apical and basolateral domains of MDCK cells. Journal of Cell Biology 111, 987-1000.[CrossRef]
    [Google Scholar]
  15. Yamamoto-Goshima, F., Maeno, K., Morishita, T., Ueda, M., Fujita, Y., Nakajima, K. & Yoshii, S. ( 1994; ). Role of neuraminidase in the morphogenesis of influenza B virus. Journal of Virology 68, 1250-1254.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-7-1729
Loading
/content/journal/jgv/10.1099/0022-1317-83-7-1729
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error