1887

Abstract

To study transmission patterns of human herpesvirus-8 (HHV-8) (Kaposi’s sarcoma-associated herpesvirus) in families in Malawi, nucleotide sequences derived from two hypervariable loci of the HHV-8 genome, the V1 and V2 regions of open reading frame K1 (K1/V1 and K1/V2, respectively), were amplified from blood and mouth rinse samples of 22 patients with treated and untreated Kaposi’s sarcoma (KS) and their first-degree relatives (=67). In patients with KS, vincristine therapy was significantly associated with non-detectability of circulating, but not oral, K1/V1 DNA. Intra-familial K1/V1 phylogenetic comparisons of eight families were possible. Both identical and non-identical sequences were observed between family members, suggesting transmission of HHV-8 along both intra- and extra-familial transmission routes.

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2002-07-01
2024-03-28
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References

  1. Athale U. H., Patil P. S., Chintu C., Elem B. 1995; Influence of HIV epidemic on the incidence of Kaposi’s sarcoma in Zambian children. Journal of Acquired Immunodeficiency Syndrome and Human Retrovirology 8:96–100
    [Google Scholar]
  2. Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noorda N. J. 1990; Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology 28:495–503
    [Google Scholar]
  3. Boshoff C., Weiss R. A. 2001; Epidemiology and pathogenesis of Kaposi’s sarcoma-associated herpesvirus. Philosophical Transactions of the Royal Society of London Biological Sciences 356:517–534
    [Google Scholar]
  4. Chang Y., Cesarman E., Pessin M. S., Lee F., Culpepper J., Knowles D. M., Moore P. S. 1994; Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi’s sarcoma. Science 266:1865–1869
    [Google Scholar]
  5. Chatlynne L. G., Lapps W., Handy M., Huang Y. Q., Masood R., Hamilton A. S., Said J. W., Koeffler H. P., Kaplan M. H., Friedman-Kien A., Gill P. S., Whitman J. E., Ablashi D. V. 1998; Detection and titration of human herpesvirus-8-specific antibodies in sera from blood donors, acquired immunodeficiency syndrome patients, and Kaposi’s sarcoma patients using a whole virus enzyme-linked immunosorbent assay. Blood 92:53–58
    [Google Scholar]
  6. Cook P. M., Whitby D., Calabro M. L., Luppi M., Kakoola D. N., Hjalgrim H., Ariyoshi K., Ensoli B., Davison A. J., Schulz T. F. 1999; Variability and evolution of Kaposi’s sarcoma-associated herpesvirus in Europe and Africa. AIDS 13:1165–1176
    [Google Scholar]
  7. Dukers N. H., Renwick N., Prins M., Geskus R. B., Schulz T. F., Weverling G. J., Coutinho R. A., Goudsmit J. 2000; Risk factors for human herpesvirus 8 seropositivity and seroconversion in a cohort of homosexual men. American Journal of Epidemiology 151:213–224
    [Google Scholar]
  8. Felsenstein J. 1993 phylip version 3.5c University of Washington; Seattle, USA:
    [Google Scholar]
  9. Gao S. J., Kingsley L., Hoover D. R., Spira T. J., Rinaldo C. R., Saah A., Phair J., Detels R., Parry P., Chang Y., Moore P. S. 1996; Seroconversion to antibodies against Kaposi′s sarcoma-associated herpesvirus-related latent nuclear antigens before the development of Kaposi’s sarcoma. New England Journal of Medicine 335:233–241
    [Google Scholar]
  10. Gessain A., Mauclere P., van Beveren M., Plancoulaine S., Ayouba A., Essame-Oyono J. L., Martin P. M., de The G. 1999; Human herpesvirus 8 primary infection occurs during childhood in Cameroon, Central Africa. International Journal of Cancer 81:189–192
    [Google Scholar]
  11. Koelle D. M., Huang M. L., Chandran B., Vieira J., Piepkorn M., Corey L. 1997; Frequent detection of Kaposi’s sarcoma-associated herpesvirus (human herpesvirus 8) DNA in saliva of human immunodeficiency virus-infected men: clinical and immunologic correlates. Journal of Infectious Diseases 176:94–102
    [Google Scholar]
  12. Lacoste V., Judde J. G., Briere J., Tulliez M., Garin B., Kassa-Kelembho E., Morvan J., Couppie P., Clyti E., Forteza V. J., Rio B., Delmer A., Mauclere P., Gessain A. 2000; Molecular epidemiology of human herpesvirus 8 in Africa: both B and A5 K1 genotypes, as well as the M and P genotypes of K14.1/K15 loci, are frequent and widespread. Virology 278:60–74
    [Google Scholar]
  13. Lampinen T. M., Kulasingam S., Min J., Borok M., Gwanzura L., Lamb J., Mahomed K., Woelk G. B., Strand K. B., Bosch M. L., Edelman D. C., Constantine N. T., Katzenstein D., Williams M. A. 2000; Detection of Kaposi’s sarcoma-associated herpesvirus in oral and genital secretions of Zimbabwean women. Journal of Infectious Diseases 181:1785–1790
    [Google Scholar]
  14. Leao J. C., Kumar N., McLean K. A., Porter S. R., Scully C. M., Swan A. V., Teo C. G. 2000; Effect of human immunodeficiency virus-1 protease inhibitors on the clearance of human herpesvirus 8 from blood of human immunodeficiency virus-1-infected patients. Journal of Medical Virology 62:416–420
    [Google Scholar]
  15. Lennette E. T., Blackbourn D. J., Levy J. A. 1996; Antibodies to human herpesvirus type 8 in the general population and in Kaposi’s sarcoma patients. Lancet 348:858–861
    [Google Scholar]
  16. Lyall E. G., Patton G. S., Sheldon J., Stainsby C., Mullen J., O’Shea S., Smith N. A., de Ruiter A., McClure M. O., Schulz T. F. 1999; Evidence for horizontal and not vertical transmission of human herpesvirus 8 in children born to human immunodeficiency virus-infected mothers. Pediatric Infectious Disease Journal 18:795–799
    [Google Scholar]
  17. Martin J. N., Ganem D. E., Osmond D. H., Page-Shafer K. A., Macrae D., Kedes D. H. 1998; Sexual transmission and the natural history of human herpesvirus 8 infection. New England Journal of Medicine 338:948–954
    [Google Scholar]
  18. Mayama S., Cuevas L. E., Sheldon J., Omar O. H., Smith D. H., Okong P., Silvel B., Hart C. A., Schulz T. F. 1998; Prevalence and transmission of Kaposi’s sarcoma-associated herpesvirus (human herpesvirus 8) in Ugandan children and adolescents. International Journal of Cancer 77:817–820
    [Google Scholar]
  19. Melbye M., Cook P. M., Hjalgrim H., Begtrup K., Simpson G. R., Biggar R. J., Ebbesen P., Schulz T. F. 1998; Risk factors for Kaposi’s-sarcoma-associated herpesvirus (KSHV/HHV-8) seropositivity in a cohort of homosexual men, 1981–1996. International Journal of Cancer 77:543–548
    [Google Scholar]
  20. Olsen S. J., Chang Y., Moore P. S., Biggar R. J., Melbye M. 1998; Increasing Kaposi’s sarcoma-associated herpesvirus seroprevalence with age in a highly Kaposi’s sarcoma endemic region, Zambia in 1985. AIDS 12:1921–1925
    [Google Scholar]
  21. Parry J. V., Connell J. A., Reinbott P., Garcia A. B., Avillez F., Mortimer P. P. 1995; GACPAT HIV 1 + 2: a simple, inexpensive assay to screen for, and discriminate between, anti-HIV 1 and anti-HIV 2. Journal of Medical Virology 45:10–16
    [Google Scholar]
  22. Pauk J., Huang M. L., Brodie S. J., Wald A., Koelle D. M., Schacker T., Celum C., Selke S., Corey L. 2000; Mucosal shedding of human herpesvirus 8 in men. New England Journal of Medicine 343:1369–1377
    [Google Scholar]
  23. Plancoulaine S., Abel L., van Beveren M., Tregouet D. A., Joubert M., Tortevoye P., de The G., Gessain A. 2000; Human herpesvirus 8 transmission from mother to child and between siblings in an endemic population. Lancet 356:1062–1065
    [Google Scholar]
  24. Simpson G. R., Schulz T. F., Whitby D., Cook P. M., Boshoff C., Rainbow L., Howard M. R., Gao S. J., Bohenzky R. A., Simmonds P., Lee C., de Ruiter A., Hatzakis A., Tedder R. S., Weller I. V., Weiss R. A., Moore P. S. 1996; Prevalence of Kaposi’s sarcoma associated herpesvirus infection measured by antibodies to recombinant capsid protein and latent immunofluorescence antigen. Lancet 348:1133–1138
    [Google Scholar]
  25. Thomas J. O. 2001; Acquired immunodeficiency syndrome-associated cancers in Sub-Saharan Africa. Seminars in Oncology 28:198–206
    [Google Scholar]
  26. Whitby D., Howard M. R., Tenant-Flowers M., Brink N. S., Copas A., Boshoff C., Hatzioannou T., Suggett F. E., Aldam D. M., Denton A. S. 1995; Detection of Kaposi sarcoma associated herpesvirus in peripheral blood of HIV-infected individuals and progression to Kaposi’s sarcoma. Lancet 346:799–802
    [Google Scholar]
  27. Ziegler J. L., Katongole-Mbidde E. 1996; Kaposi’s sarcoma in childhood: an analysis of 100 cases from Uganda and relationship to HIV infection. International Journal of Cancer 65:200–203
    [Google Scholar]
  28. Zong J. C., Ciufo D. M., Alcendor D. J., Wan X., Nicholas J., Browning P. J., Rady P. L., Tyring S. K., Orenstein J. M., Rabkin C. S., Su I. J., Powell K. F., Croxson M., Foreman K. E., Nickoloff B. J., Alkan S., Hayward G. S. 1999; High-level variability in the ORF-K1 membrane protein gene at the left end of the Kaposi’s sarcoma-associated herpesvirus genome defines four major virus subtypes and multiple variants or clades in different human populations. Journal of Virology 73:4156–4170
    [Google Scholar]
  29. Zong J., Ciufo D. M., Viscidi R., Alagiozoglou L., Tyring S., Rady P., Orenstein J., Boto W., Kalumbuja H., Romano N., Melbye M., Kang G. H., Boshoff C., Hayward G. S. 2002; Genotypic analysis at multiple loci across Kaposi’s sarcoma herpesvirus (KSHV) DNA molecules: clustering patterns, novel variants and chimerism. Journal of Clinical Virology 23:119–148
    [Google Scholar]
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