1887

Abstract

Co-inoculation of plants with transcripts of both genomic and short defective interfering (DI) RNAs of results in an accumulation of generated DI RNA dimers. Time-course analysis of DI RNA accumulation in the inoculated leaves showed early accumulation of DI RNA dimers followed by increased levels of DI RNA monomers. In contrast, DI RNA dimers were barely detectable in systems where cell-to-cell movement does not take place (protoplasts) or is less important (monomeric DI RNA-expressing transgenic plants). Our results also demonstrated that the size of DI RNAs is important in the colonization of inoculated leaves, suggesting that DI RNA dimers are quickly selected for cell-to-cell movement if short DI RNA monomers are used for infection.

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2002-06-01
2020-09-18
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References

  1. Burgyán J., Rubino L., Russo M.. 1991; De novo generation of Cymbidium ringspot virus defective interfering RNA. Journal of General Virology72:505–509
    [Google Scholar]
  2. Burgyán J., Hornyik Cs., Szittya Gy., Silhavy D., Bisztray G.. 2000; The ORF1 products of tombusviruses play a crucial role in lethal necrosis of virus-infected plants. Journal of Virology74:10873–10881
    [Google Scholar]
  3. Carpenter C. D., Cascone P. J., Simon A. E.. 1991; Formation of multimers of linear satellite RNAs. Virology183:586–594
    [Google Scholar]
  4. Carrington J. C., Kasschau K. D., Mahajan S. K., Schaad C. M.. 1996; Cell-to-cell and long-distance transport of viruses in plants. Plant Cell8:1669–1681
    [Google Scholar]
  5. Dalmay T., Rubino L., Burgyán J., Russo M.. 1992; Replication and movement of a coat protein mutant of cymbidium ringspot tombusvirus. Molecular Plant–Microbe Interactions5:379–383
    [Google Scholar]
  6. Dalmay T., Rubino L., Burgyán J., Kollár Á., Russo M.. 1993; Functional analysis of cymbidium ringspot virus genome. Virology194:697–704
    [Google Scholar]
  7. Dalmay T., Szittya Gy., Burgyán J.. 1995; Generation of defective interfering RNA dimers of cymbidium ringspot tombusvirus. Virology207:510–517
    [Google Scholar]
  8. Finnen R. L., Rochon D. M.. 1995; Characterization and biological activity of DI RNA dimers formed during cucumber necrosis virus coinfections. Virology207:282–286
    [Google Scholar]
  9. Havelda Z., Dalmay T., Burgyán J.. 1995; Localization of cis -acting sequences essential for cymbidium ringspot tombusvirus defective interfering RNA replication. Journal of General Virology76:2311–2316
    [Google Scholar]
  10. Havelda Z., Dalmay T., Burgyán J.. 1997; Secondary structure-dependent evolution of cymbidium ringspot virus defective interfering RNA. Journal of General Virology78:1227–1234
    [Google Scholar]
  11. Inoue-Nagata A. K., Kormelink R., Sgro J. Y., Nagata T., Kitajima E. W., Goldbach R., Peters D.. 1998; Molecular characterization of tomato spotted wilt virus defective interfering RNAs and detection of truncated L proteins. Virology248:342–356
    [Google Scholar]
  12. Kollár Á., Burgyán J.. 1994; Evidence that ORF 1 and 2 are the only virus encoded replicase gene of cymbidium ringspot tombusvirus. Virology201:169–172
    [Google Scholar]
  13. Kollár Á., Dalmay T., Burgyán J.. 1993; Defective interfering RNA-mediated resistance against cymbidium ringspot tombusvirus. Virology193:313–318
    [Google Scholar]
  14. Kuroda T., Natsuaki T., Wang W.-Q., Okuda S.. 1997; Formation of multimers of cucumber mosaic virus satellite RNA. Journal of General Virology78:941–946
    [Google Scholar]
  15. Lucas W. J., Wolf S.. 1999; Connections between virus movement, macromolecular signaling and assimilate allocation. Current Opinion in Plant Biology2:192–197
    [Google Scholar]
  16. Nagy P. D., Pogany J., Simon A. E.. 1999; RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus-strand RNA virus. EMBO Journal18:5653–5665
    [Google Scholar]
  17. Oster S. K., Wu B., White A.. 1998; Uncoupled expression of p33 and p92 permits amplification of tomato bushy stunt virus RNAs. Journal of Virology72:5845–5851
    [Google Scholar]
  18. Rochon D. M., Johnston J. C.. 1991; Infectious transcripts from cloned cucumber necrosis virus cDNA. Evidence for a bifunctional subgenomic mRNA. Virology181:656–665
    [Google Scholar]
  19. Rochon D. M., Finnen R. L., Sit T. L.. 1994; Coat protein of cucumber necrosis virus is not required for efficient generation or accumulation of defective interfering RNAs. Journal of General Virology75:2505–2508
    [Google Scholar]
  20. Roux L., Simon A. E., Holland J. J.. 1991; Effects of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo . Advances in Virus Research40:181–211
    [Google Scholar]
  21. Russo M., Burgyán J., Martelli P. G.. 1994; The molecular biology of Tombusviridae. Advances in Virus Research44:382–424
    [Google Scholar]
  22. Scholthof H. B., Scholthof K.-B. G., Jackson A. O.. 1995a; Identification of tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector. Plant Cell7:1157–1172
    [Google Scholar]
  23. Scholthof H. B., Scholthof K.-B. G., Kikkert M., Jackson A. O.. 1995b; Tomato bushy stunt virus spread is regulated by two nested genes that function in cell-to-cell movement and host-dependent systemic invasion. Virology213:425–438
    [Google Scholar]
  24. Tzfira T., Rhee Y., Chen M. H., Kunik T., Citovsky V.. 2000; Nucleic acid transport in plant-microbe interactions: the molecules that walk through the walls. Annual Review of Microbiology54:187–219
    [Google Scholar]
  25. Voinnet O., Pinto Y. M., Baulcombe D. C.. 1999; Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proceedings of the National Academy of Sciences, USA96:14147–14152
    [Google Scholar]
  26. White K. A.. 1996; Formation and evolution of Tombusvirus defective interfering RNAs. Seminars in Virology7:409–416
    [Google Scholar]
  27. White K. A., Morris T. J.. 1999; Defective and defective interfering RNAs of monopartite plus-strand RNA plant viruses. Current Topics in Microbiology and Immunology239:1–17
    [Google Scholar]
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