1887

Abstract

The complete nucleotide sequences of genomic segments S1, S3 and S4 from Bombyx mori cypovirus 1 (BmCPV-1) have been determined. The segments consisted of 4190, 3846 and 3262 nucleotides encoding putative proteins of 1333, 1239 and 1058 amino acids with molecular masses of approximately 148, 140 and 120 kDa (p148, p140 and p120, respectively). All segments possess a single open reading frame. Homology searches showed that all three proteins have homologies to proteins of , a member of the genus within the family . Partial homologies of p140 to structural proteins in other viruses were also found. The predicted molecular masses and the homologies with structural proteins in other viruses lead us to suggest that S1, S3 and S4 encode the capsid proteins VP1, VP3, and VP4, respectively, of BmCPV-1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-6-1477
2002-06-01
2020-08-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/6/0831477a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-6-1477&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research25:3389–3402
    [Google Scholar]
  2. Belloncik S., Liu J., Su D., Arella M.. 1996; Identification and characterization of a new Cypovirus , type 14, isolated from Heliothis armigera . Journal of Invertebrate Pathology67:41–47
    [Google Scholar]
  3. Chou P. Y., Fasman G. D.. 1974; Prediction of protein conformation. Biochemistry13:222–245
    [Google Scholar]
  4. Chou P. Y., Fasman G. D.. 1978; Empirical predictions of protein conformation. Annual Review of Biochemistry47:251–276
    [Google Scholar]
  5. Fouillaud M., Morel G.. 1994; Characterization of cytoplasmic and nuclear polyhedrosis viruses recovered from the nest of Polistes hebraeus F. (Hymenoptera; Vespidae) . Journal of Invertebrate Pathology42:401–404
    [Google Scholar]
  6. Fujii-Kawata I., Miura K.-I., Fuke M.. 1970; Segments of genome of viruses containing double-stranded ribonucleic acid. Journal of Molecular Biology51:247–253
    [Google Scholar]
  7. Hagiwara K., Matsumoto T.. 2000; Nucleotide sequences of genome segments 6 and 7 of Bombyx mori cypovirus 1, encoding the viral structural proteins V4 and V5, respectively. Journal of General Virology81:1143–1147
    [Google Scholar]
  8. Hagiwara K., Tomita M., Kobayashi J., Miyajima S., Yoshimura T.. 1998a; Nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 8. Biochemical and Biophysical Research Communications247:549–553
    [Google Scholar]
  9. Hagiwara K., Tomita M., Nakai K., Kobayashi J., Miyajima S., Yoshimura T.. 1998b; Determination of the nucleotide sequence of Bombyx mori cytoplasmic polyhedrosis virus segment 9 and its expression in BmN4 cells. Journal of Virology72:5762–5768
    [Google Scholar]
  10. Hagiwara K., Kobayashi J., Tomita M., Yoshimura T.. 2001; Nucleotide sequence of genome segment 5 from Bombyx mori cypovirus 1. Archives of Virology146:181–187
    [Google Scholar]
  11. Holmes I. H., Boccardo G., Estes M. K., Furuichi M. K., Hoshino Y., Joklik W. K., McCrae M., Mertens P. P. C., Milne G., Samal K. S. K., Shikata E., Winton J. R., Uyeda I.. 1995; Family Reoviridae . In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp208–237 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D.. Vienna & New York: Springer-Verlag;
    [Google Scholar]
  12. Hom L. G., Volkman L. E.. 2000; Autographa californica M nucleopolyhedrovirus chiA is required for processing of V-CATH. Virology277:178–183
    [Google Scholar]
  13. Hukuhara T., Midorikawa M.. 1983; Pathogenesis of cytoplasmic polyhedrosis in the silkworm. In Double-stranded RNA Viruses pp405–414 Edited by Compans R. W., Bishop D. H. L.. New York: Elsevier Science;
    [Google Scholar]
  14. Ikeda K., Nagaoka S., Winkler S., Kotani K., Yagi H., Nakanishi K., Miyajima S., Kobayashi J., Mori H.. 2001; Molecular characterization of Bombyx mori cytoplasmic polyhedrosis virus genome segment 4. Journal of Virology75:988–995
    [Google Scholar]
  15. Kang W., Tristem M., Maeda S., Crook N. E., O’Reilly D. R.. 1998; Identification and characterization of the Cydia pomonella granulovirus cathepsin and chitinase genes. Journal of General Virology79:2283–2292
    [Google Scholar]
  16. McCrae M. A., Mertens P. P. C.. 1983; In vitro translation studies on and RNA coding assignments for cytoplasmic polyhedrosis viruses. In Double-stranded RNA Viruses pp35–41 Edited by Compans R. W., Bishop D. H. L.. New York: Elsevier Science;
    [Google Scholar]
  17. Miyajima S., Mori A., Hagiwara K., Kobayashi J., Yoshimura T., Nakai K., Suzuki Y., Tomita M.. 1998; Microheterogeneity in the polyhedrin gene of Bombyx mori , a cytoplasmic polyhedrosis virus. Journal of Sericultural Science of Japan67:287–294
    [Google Scholar]
  18. Payne C. C., Mertens P. P. C.. 1983; Cytoplasmic polyhedrosis viruses. In The Reoviridae pp425–504 Edited by Joklik W. K.. New York: Academic Press;
    [Google Scholar]
  19. Payne C. C., Rivers C. F.. 1976; A provisional classification of cytoplasmic polyhedrosis viruses based on the sizes of the RNA genome segments. Journal of General Virology33:71–85
    [Google Scholar]
  20. Thomas C. J., Brown H. L., Hawes C. R., Lee B. Y., Min M.-K., King L. A., Possee R. D.. 1998; Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum. Journal of Virology72:10207–10212
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-6-1477
Loading
/content/journal/jgv/10.1099/0022-1317-83-6-1477
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error