1887

Abstract

During hepatitis B virus (HBV) infection, high numbers of non-infectious HBV surface antigen (HBsAg) particles are present in circulation. It is shown here that recombinant HBsAg (rHBsAg) particles, which contain the S protein only, bind almost exclusively to monocytes. Attachment of rHBsAg to the THP-1 pre-monocytic cell line occurs upon 1,25-dihydroxyvitamin D3-induced differentiation. Binding to monocytes is enhanced by a heat-labile serum protein and is inhibited by Ca/Mg, low pH and an HBsAg-specific monoclonal antibody. Furthermore, it is shown that rHBsAg suppresses lipopolysaccharide- and IL-2-induced production of cytokines. These results suggest the existence of a monocyte-specific receptor, the engagement of which by HBsAg suppresses the activity of these cells.

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2002-06-01
2024-12-13
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References

  1. Alcami A., Koszinowski U. H. 2000; Viral mechanisms of immune evasion. Immunology Today 21:447–455
    [Google Scholar]
  2. Akbar S. M., Onji M., Inaba K., Yamamura K.-I., Ohta Y. 1993; Low responsiveness of hepatitis B virus-transgenic mice in antibody response to T-cell-dependent antigen: defect in antigen-presenting activity of dendritic cells. Immunology 78:468–475
    [Google Scholar]
  3. Bosco M. C., Espinoza-Delgado I., Rowe T. K., Malabarba M. G., Longo D. L., Varesio L. 1997; Functional role for the myeloid differentiation antigen CD14 in the activation of human monocytes by IL-2. Journal of Immunology 159:2922–2931
    [Google Scholar]
  4. Budkowska A., Quan C., Groh F., Bedossa P., Dubreuil P., Bouvet J. P., Pillot J. 1993; Hepatitis B virus (HBV) binding factor in human serum: candidate for a soluble form of hepatocyte HBV receptor. Journal of Virology 67:4316–4322
    [Google Scholar]
  5. Chisari F. V. 1995; Hepatitis B virus transgenic mice: insights into the virus and the disease. Hepatology 22:1316–1325
    [Google Scholar]
  6. Chisari F. V., Ferrari C. 1995; Hepatitis B virus immunopathogenesis. Annual Reviews in Immunology 13:29–60
    [Google Scholar]
  7. Gagliardi M. C., Nisini R., Benvenuto R., De Petrillo G., Michel M. L., Barnaba V. 1994; Soluble transferrin mediates targeting of hepatitis B envelope antigen to transferrin receptor and its presentation by activated T cells. European Journal of Immunology 24:1372–1376
    [Google Scholar]
  8. Ganem D. 1996; Hepadnaviridae and their replication. In Fields Virology pp 2703–2738 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  9. Imai M., Yanase Y., Nojiri T., Miyakawa Y., Mayumi M. 1979; A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology 76:242–247
    [Google Scholar]
  10. Jochum C., Voth R., Rossol S., Meyer zum Buschenfelde K. H., Hess G., Will H., Schroder H. C., Steffen R., Muller W. E. 1990; Immunosuppressive function of hepatitis B antigens in vitro : role of endoribonuclease V as one potential trans inactivator for cytokines in macrophages and human hepatoma cells. Journal of Virology 64:1956–1963
    [Google Scholar]
  11. Komai K., Peeples M. E. 1990; Physiology and function of the vero cell receptor for the hepatitis B virus small S protein. Virology 177:332–338
    [Google Scholar]
  12. Kurose K., Akbar S. M., Yamamoto K., Onji M. 1997; Production of antibody to hepatitis B surface antigen (anti-HBs) by murine hepatitis B virus carriers: neonatal tolerance versus antigen presentation by dendritic cells. Immunology 92:494–500
    [Google Scholar]
  13. Mehdi M., Kaplan M. J., Anlar F. Y., Yang X., Bayer R., Sutherland K., Peeples M. E. 1994; Hepatitis B virus surface antigen binds to apolipoprotein H. Journal of Virology 68:2415–2424
    [Google Scholar]
  14. Mehdi H., Yang X., Peeples M. E. 1996; An altered form of apolipoprotein H binds hepatitis B virus surface antigen most efficiently. Virology 217:58–66
    [Google Scholar]
  15. Milich D. R. 1997; Pathobiology of acute and chronic hepatitis B virus infection: an introduction. Journal of Viral Hepatitis 4:25–30
    [Google Scholar]
  16. Muller C., Zielinski C. C. 1990; Impaired lipopolysaccharide-inducible tumor necrosis factor production in vitro by peripheral blood monocytes of patients with viral hepatitis. Hepatology 12:1118–1124
    [Google Scholar]
  17. Muller C., Zielinski C. C. 1992; Interleukin-6 production by peripheral blood monocytes in patients with chronic liver disease and acute viral hepatitis. Journal of Hepatology 15:372–377
    [Google Scholar]
  18. Neurath A. R., Strick N., Sproul P. 1992; Search for hepatitis B virus cell receptors reveals binding sites for interleukin 6 on the virus envelope protein. Journal of Experimental Medicine 175:461–469
    [Google Scholar]
  19. Paulij W. P., de Wit P. L. M., Sünnen C. M. G., van Roosmalen M. H., Petersen-van Ettekoven A., Cooreman M. P., Heijtink R. A. 1999; Localization of a unique hepatitis B virus epitope sheds new light on the structure of hepatitis B virus surface antigen. Journal of General Virology 80:2121–2126
    [Google Scholar]
  20. Pedron T., Girard R., Chaby R. 1995; Variation of LPS-binding capacity, epitope expression, and shedding of membrane-bound CD14 during differentiation of human monocytes. Journal of Immunology 155:1460–1471
    [Google Scholar]
  21. Pontisso P., Morsica G., Ruvoletto M. G., Zambello R., Colletta C., Chemello L., Alberti A. 1991; Hepatitis B virus binds to peripheral blood mononuclear cells via the pre S1 protein. Journal of Hepatology 12:203–206
    [Google Scholar]
  22. Stirk H. J., Thornton J. M., Howard C. R. 1992; A topological model for hepatitis B surface antigen. Intervirology 33:148–158
    [Google Scholar]
  23. Stricker E. A. M., Tiebout R. F., Lelie P. N., Zeijlemaker W. P. 1985; A human monoclonal IgG1λ anti-hepatitis B surface antibody. Production, properties, and application. Scandinavian Journal of Immunology 22:337–343
    [Google Scholar]
  24. Tortorella D., Gewurz B. E., Furman M. H., Schust D. J., Ploegh H. L. 2000; Viral subversion of the immune system. Annual Review of Immunology 18:861–926
    [Google Scholar]
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