1887

Abstract

During hepatitis B virus (HBV) infection, high numbers of non-infectious HBV surface antigen (HBsAg) particles are present in circulation. It is shown here that recombinant HBsAg (rHBsAg) particles, which contain the S protein only, bind almost exclusively to monocytes. Attachment of rHBsAg to the THP-1 pre-monocytic cell line occurs upon 1,25-dihydroxyvitamin D3-induced differentiation. Binding to monocytes is enhanced by a heat-labile serum protein and is inhibited by Ca/Mg, low pH and an HBsAg-specific monoclonal antibody. Furthermore, it is shown that rHBsAg suppresses lipopolysaccharide- and IL-2-induced production of cytokines. These results suggest the existence of a monocyte-specific receptor, the engagement of which by HBsAg suppresses the activity of these cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-6-1281
2002-06-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/6/0831281a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-6-1281&mimeType=html&fmt=ahah

References

  1. Alcami, A. & Koszinowski, U. H. ( 2000; ). Viral mechanisms of immune evasion. Immunology Today 21, 447-455.[CrossRef]
    [Google Scholar]
  2. Akbar, S. M., Onji, M., Inaba, K., Yamamura, K.-I. & Ohta, Y. ( 1993; ). Low responsiveness of hepatitis B virus-transgenic mice in antibody response to T-cell-dependent antigen: defect in antigen-presenting activity of dendritic cells. Immunology 78, 468-475.
    [Google Scholar]
  3. Bosco, M. C., Espinoza-Delgado, I., Rowe, T. K., Malabarba, M. G., Longo, D. L. & Varesio, L. ( 1997; ). Functional role for the myeloid differentiation antigen CD14 in the activation of human monocytes by IL-2. Journal of Immunology 159, 2922-2931.
    [Google Scholar]
  4. Budkowska, A., Quan, C., Groh, F., Bedossa, P., Dubreuil, P., Bouvet, J. P. & Pillot, J. ( 1993; ). Hepatitis B virus (HBV) binding factor in human serum: candidate for a soluble form of hepatocyte HBV receptor. Journal of Virology 67, 4316-4322.
    [Google Scholar]
  5. Chisari, F. V. ( 1995; ). Hepatitis B virus transgenic mice: insights into the virus and the disease. Hepatology 22, 1316-1325.
    [Google Scholar]
  6. Chisari, F. V. & Ferrari, C. ( 1995; ). Hepatitis B virus immunopathogenesis. Annual Reviews in Immunology 13, 29-60.[CrossRef]
    [Google Scholar]
  7. Gagliardi, M. C., Nisini, R., Benvenuto, R., De Petrillo, G., Michel, M. L. & Barnaba, V. ( 1994; ). Soluble transferrin mediates targeting of hepatitis B envelope antigen to transferrin receptor and its presentation by activated T cells. European Journal of Immunology 24, 1372-1376.[CrossRef]
    [Google Scholar]
  8. Ganem, D. ( 1996; ). Hepadnaviridae and their replication. In Fields Virology , pp. 2703-2738. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia:Lippincott–Raven.
  9. Imai, M., Yanase, Y., Nojiri, T., Miyakawa, Y. & Mayumi, M. ( 1979; ). A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology 76, 242-247.
    [Google Scholar]
  10. Jochum, C., Voth, R., Rossol, S., Meyer zum Buschenfelde, K. H., Hess, G., Will, H., Schroder, H. C., Steffen, R. & Muller, W. E. ( 1990; ). Immunosuppressive function of hepatitis B antigens in vitro: role of endoribonuclease V as one potential trans inactivator for cytokines in macrophages and human hepatoma cells. Journal of Virology 64, 1956-1963.
    [Google Scholar]
  11. Komai, K. & Peeples, M. E. ( 1990; ). Physiology and function of the vero cell receptor for the hepatitis B virus small S protein. Virology 177, 332-338.[CrossRef]
    [Google Scholar]
  12. Kurose, K., Akbar, S. M., Yamamoto, K. & Onji, M. ( 1997; ). Production of antibody to hepatitis B surface antigen (anti-HBs) by murine hepatitis B virus carriers: neonatal tolerance versus antigen presentation by dendritic cells. Immunology 92, 494-500.[CrossRef]
    [Google Scholar]
  13. Mehdi, M., Kaplan, M. J., Anlar, F. Y., Yang, X., Bayer, R., Sutherland, K. & Peeples, M. E. ( 1994; ). Hepatitis B virus surface antigen binds to apolipoprotein H. Journal of Virology 68, 2415-2424.
    [Google Scholar]
  14. Mehdi, H., Yang, X. & Peeples, M. E. ( 1996; ). An altered form of apolipoprotein H binds hepatitis B virus surface antigen most efficiently. Virology 217, 58-66.[CrossRef]
    [Google Scholar]
  15. Milich, D. R. ( 1997; ). Pathobiology of acute and chronic hepatitis B virus infection: an introduction. Journal of Viral Hepatitis 4, 25-30.[CrossRef]
    [Google Scholar]
  16. Muller, C. & Zielinski, C. C. ( 1990; ). Impaired lipopolysaccharide-inducible tumor necrosis factor production in vitro by peripheral blood monocytes of patients with viral hepatitis. Hepatology 12, 1118-1124.[CrossRef]
    [Google Scholar]
  17. Muller, C. & Zielinski, C. C. ( 1992; ). Interleukin-6 production by peripheral blood monocytes in patients with chronic liver disease and acute viral hepatitis. Journal of Hepatology 15, 372-377.[CrossRef]
    [Google Scholar]
  18. Neurath, A. R., Strick, N. & Sproul, P. ( 1992; ). Search for hepatitis B virus cell receptors reveals binding sites for interleukin 6 on the virus envelope protein. Journal of Experimental Medicine 175, 461-469.[CrossRef]
    [Google Scholar]
  19. Paulij, W. P., de Wit, P. L. M., Sünnen, C. M. G., van Roosmalen, M. H., Petersen-van Ettekoven, A., Cooreman, M. P. & Heijtink, R. A. ( 1999; ). Localization of a unique hepatitis B virus epitope sheds new light on the structure of hepatitis B virus surface antigen. Journal of General Virology 80, 2121-2126.
    [Google Scholar]
  20. Pedron, T., Girard, R. & Chaby, R. ( 1995; ). Variation of LPS-binding capacity, epitope expression, and shedding of membrane-bound CD14 during differentiation of human monocytes. Journal of Immunology 155, 1460-1471.
    [Google Scholar]
  21. Pontisso, P., Morsica, G., Ruvoletto, M. G., Zambello, R., Colletta, C., Chemello, L. & Alberti, A. ( 1991; ). Hepatitis B virus binds to peripheral blood mononuclear cells via the pre S1 protein. Journal of Hepatology 12, 203-206.[CrossRef]
    [Google Scholar]
  22. Stirk, H. J., Thornton, J. M. & Howard, C. R. ( 1992; ). A topological model for hepatitis B surface antigen. Intervirology 33, 148-158.
    [Google Scholar]
  23. Stricker, E. A. M., Tiebout, R. F., Lelie, P. N. & Zeijlemaker, W. P. ( 1985; ). A human monoclonal IgG1λ anti-hepatitis B surface antibody. Production, properties, and application. Scandinavian Journal of Immunology 22, 337-343.[CrossRef]
    [Google Scholar]
  24. Tortorella, D., Gewurz, B. E., Furman, M. H., Schust, D. J. & Ploegh, H. L. ( 2000; ). Viral subversion of the immune system. Annual Review of Immunology 18, 861-926.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-6-1281
Loading
/content/journal/jgv/10.1099/0022-1317-83-6-1281
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error