1887

Abstract

The positive effect of the co-expression of T helper (Th) cell type 2 cytokine interleukin-5 (IL-5) on -deleted simian/human immunodeficiency virus (SHIV) replication has been observed previously. To analyse whether the growth advantage of IL-5-containing SHIV (NI-IL5) would be relevant , the virus was inoculated into monkeys. Three rhesus macaques were inoculated intravenously with 10 TCID of NI-IL5. Results were compared with those obtained previously from SHIV NM-3rN (intact) and SHIV-dn (-deleted)-infected monkeys. Cytokine production, analysed by IL-5 ELISA, showed a twofold increase in IL-5 concentration in the plasma soon after the peak of virus replication. Virus replication and antibody production were greater in monkeys inoculated with IL-5-expressing SHIV than in monkeys inoculated with -deleted SHIV without IL-5. These findings show a stimulation of SHIV replication by co-expression of IL-5 and suggest the important role of Th2-type cytokines in human immunodeficiency virus type 1 infection.

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2002-05-01
2020-08-05
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