1887

Abstract

Nine different CTL epitopes, conserved in both Hantaan virus (HTNV) and Sin Nombre virus (SNV), were selected for study. The binding affinity of each peptide with HLA-A2.1 molecules was determined and antigen-specific responses from seven donors who had a previous field infection with HTNV were examined. Although the strength or frequency of CTL activity showed different patterns in the seven patients, five of seven patients showed significant activity against at least one or more epitope peptides. In particular, the peptide ILQDMRNTI (HTNV, aa 334–342; SNV, aa 333–341), which elicited CTL activity in five patients, was shown to be specifically HLA-A2.1-restricted in partially cloned CD8 T cells and also induced activated and effector CD8 T cell-producing T cytotoxic (Tc) type 1 cytokines, such as IL-2 and IFN-γ. The results suggest that this epitope would serve as a useful component for the intervention of both HTNV and SNV infection.

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2002-05-01
2024-03-29
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References

  1. Belz G. T., Xie W., Doherty P. C. 2001; Diversity of epitope and cytokine profiles for primary and secondary influenza A virus-specific CD8+ T cell responses. Journal of Immunology 166:4627–4633
    [Google Scholar]
  2. Butterfield L. H., Meng W. S., Koh A., Vollmer C. M., Ribas A., Dissette V. B., Faull K., Glaspy J. A., McBride W. H., Economou J. S. 2001; T cell responses to HLA-A*0201-restricted peptides derived from human alpha fetoprotein. Journal of Immunology 166:5300–5308
    [Google Scholar]
  3. Detels R., Mann D., Carrington M., Hennessey K., Wu Z., Hirji K. F., Wiley D., Visscher B. R., Giorgi J. V. 1996; Persistently seronegative men from whom HIV-1 has been isolated are genetically and immunologically distinct. Immunology Letters 51:29–33
    [Google Scholar]
  4. Ennis F. A., Cruz J., Spiropoulou C. F., Waite D., Peters C. J., Nichol S. T., Kariwa H., Koster F. T. 1997; Hantavirus pulmonary syndrome: CD8+ and CD4+ cytotoxic T lymphocytes to epitopes on Sin Nombre virus nucleocapsid protein isolated during acute illness. Virology 238:380–390
    [Google Scholar]
  5. Huang C., Jin B., Wang M., Li E., Sun C. 1994; Hemorrhagic fever with renal syndrome: relationship between pathogenesis and cellular immunity. Journal of Infectious Diseases 169:868–870
    [Google Scholar]
  6. Koster F. T., Williams T. M., Griffith B. B., Goade D. E., Hjelle B. L. 1998; Genetic associations with hantavirus pulmonary syndrome due to Sin Nombre virus. In Fourth International Conference on HFRS and Hantavirus p–179 Atlanta, GA: American Society for Tropical Medicine and Hygiene;
    [Google Scholar]
  7. Krakauer T., Leduc J. W., Morrill J. C., Anderson A. O., Krakauer H. 1994; Serum levels of alpha and gamma interferons in hemorrhagic fever with renal syndrome. Viral Immunology 7:97–101
    [Google Scholar]
  8. Lee H. G., Lim J. S., Lee K. Y., Choi Y. K., Choe I. S., Chung T. W., Kim K. 1997; Peptide-specific CTL induction in HBV-seropositive PBMC by stimulation with peptides in vitro : novel epitopes identified from chronic carriers. Virus Research 50:185–194
    [Google Scholar]
  9. Levy F., Gabathuler R., Larsson R., Kvist S. 1991; ATP is required for in vitro assembly of MHC class I antigens but not for transfer of peptides across the ER membrane. Cell 67:265–274
    [Google Scholar]
  10. Linderholm M., Ahlm C., Settergren B., Waage A., Tarnvik A. 1996; Elevated plasma levels of tumor necrosis factor (TNF)-α, soluble TNF receptors, interleukin (IL)-6, and IL-10 in patients with hemorrhagic fever with renal syndrome. Journal of Infectious Diseases 173:38–43
    [Google Scholar]
  11. Lopez N., Padula P., Rossi C., Lazaro M. E., Franze-Fernandez M. T. 1996; Genetic identification of a new hantavirus causing severe pulmonary syndrome in Argentina. Virology 220:223–226
    [Google Scholar]
  12. Minev B. R., Chavez F. L., Dudouet B. M., Mitchell M. S. 2000; Synthetic insertion signal sequences enhance MHC class I presentation of a peptide from the melanoma antigen MART-1. European Journal of Immunology 30:2115–2124
    [Google Scholar]
  13. Mortara L., Letourneur F., Villefroy P., Beyer C., Gras-Masse H., Guillet J. G., Bourgault-Villada I. 2000; Temporal loss of Nef-epitope CTL recognition following macaque lipopeptide immunization and SIV challenge. Virology 278:551–561
    [Google Scholar]
  14. Mosmann T. R., Sad S. 1996; The expanding universe of T-cell subsets: Th1, Th2 and more. Immunology Today 17:138–146
    [Google Scholar]
  15. Nichol S. T., Spiropoulou C. F., Morzunov S., Rollin P. E., Ksiazek T. G., Feldmann H., Sanchez A., Childs J., Zaki S., Peters C. J. 1993; Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science 262:914–917
    [Google Scholar]
  16. Parker K. C., Bednarek M. A., Coligan J. E. 1994; Scheme for ranking potential HLA-A2 binding peptide based on independent binding of individual peptide side-chains. Journal of Immunology 152:163–175
    [Google Scholar]
  17. Romagnani S. 1997; The Th1/Th2 paradigm. Immunology Today 18:263–266
    [Google Scholar]
  18. Rutella S., Rumi C., Lucia M. B., Etuk B., Cauda R., Leone G. 1998; Flow cytometric detection of perforin in normal human lymphocyte subpopulations defined by expression of activation/differentiation antigens. Immunology Letters 60:51–55
    [Google Scholar]
  19. Sing G. K., Ladhams A., Arnold S., Parmar H., Chen X., Cooper J., Butterworth L., Stuart K., D’Arcy D., Cooksley W. G. 2000; A longitudinal analysis of cytotoxic T lymphocyte precursor frequencies to the hepatitis B virus in chronically infected patients. Journal of Viral Hepatitis 8:19–29
    [Google Scholar]
  20. Temonen M., Mustonen J., Helin H., Pasternack A., Vaheri A., Holthofer H. 1996; Cytokines, adhesion molecules, and cellular infiltration in nephropathia epidemica kidneys: an immunohistochemical study. Clinical Immunology and Immunopathology 78:47–55
    [Google Scholar]
  21. Tourdot S., Herath S., Gould K. G. 2001; Characterization of a new H-2Dk-restricted epitope prominent in primary influenza A virus infection. Journal of General Virology 82:1749–1755
    [Google Scholar]
  22. Van Epps H. L., Schmaljohn C. S., Ennis F. A. 1999; Human memory cytotoxic T-lymphocyte (CTL) responses to Hantaan virus infection: identification of virus-specific and cross-reactive CD8+ CTL epitopes on nucleocapsid protein. Journal of Virology 73:5301–5308
    [Google Scholar]
  23. Williams R. J., Bryan R. T., Mills J. N., Palma R. E., Vera I., De Velasquez F., Baez E., Schmidt W. E., Figueroa R. E., Peters C. J., Zaki S. R., Khan A. S., Ksiazek T. G. 1997; An outbreak of hantavirus pulmonary syndrome in western Paraguay. American Journal of Tropical Medicine and Hygiene 57:274–282
    [Google Scholar]
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