1887

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Volume 83, Issue 5

Non Fc receptor-mediated infection of human macrophages by dengue virus serotype 2 Free

Abstract

Four human monocyte-derived macrophage membrane proteins, with apparent molecular masses of 27, 45, 67 and 87 kDa, were identified as possible receptors for dengue virus serotype 2 (DEN-2) (Mexican isolate 200787/1983), based on affinity chromatography, immunofluorescence, virus overlay protein-binding assays and Western blotting. Additionally, mouse polyclonal antibodies raised against each of the four proteins were capable of partially inhibiting DEN-2 infection of monocyte-macrophages, thus supporting the notion of a role for such proteins as DEN-2 receptors. Parallel studies were carried out using the human promonocytic U-937 cell line, both as undifferentiated cells and as monocyte-like phorbol myristate acetate (PMA)-differentiated cells, as target cells. Whereas interaction between DEN-2 and undifferentiated U-937 cells was almost negligible, PMA-differentiated U-937 cells were shown to harbour putative receptors (with molecular masses of 45 and 67 kDa) for DEN-2, similar to those found in human monocyte-derived macrophages. To our knowledge, this is the first report that describes putative receptors for DEN-2 in primary cultures of human macrophages.

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© Microbiology Society

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An erratum has been published for this content:
Non Fc receptor-mediated infection of human macrophages by dengue virus serotype 2
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2002-05-01
2024-04-24
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Non Fc receptor-mediated infection of human macrophages by dengue virus serotype 2
J. Gen. Virol. 83, 1123 (2002); https://doi.org/10.1099/0022-1317-83-5-1123
/content/journal/jgv/10.1099/0022-1317-83-5-1123
/content/journal/jgv/10.1099/0022-1317-83-5-1123
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