1887

Abstract

Among the important challenges to shrimp aquaculture worldwide are the diseases caused by viruses, in particular by white spot syndrome virus (WSSV), which has a genome estimated to contain 305 kb. By analysis and comparison of the WSSV genomic DNA and cDNA libraries, an ORF ( gene) was identified. The gene, encoding a novel 204-amino-acid protein, was expressed in and purified. A specific antibody was raised using the purified VP28 protein. After inoculation of healthy adult shrimp with WSSV, the gene transcript and VP28 protein were first detected at low levels at 6 and 18 h post-infection, respectively. These experiments suggest that it might be a late gene. Immuno-electron microscopy with gold-labelled antibody revealed that the gold particles were distributed in the outer envelope of WSSV virions and showed that encodes a virus envelope protein.

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/content/journal/jgv/10.1099/0022-1317-83-5-1069
2002-05-01
2019-10-20
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