1887

Abstract

Genes encoding two representatives of the LD130 family of baculovirus envelope-associated proteins were transcriptionally mapped. These included , which encodes a low pH-induced envelope fusion protein of the multinucleocapsid nucleopolyhedrovirus, and , which is related to but is encoded by MNPV and appears to lack an envelope fusion activity. The size and temporal expression of mRNA of both genes were examined by Northern blot analysis of RNA extracted from infected cells at selected timepoints. In addition, 5′ rapid amplification of cDNA ends (RACE) in combination with DNA sequence analysis was used to map the start sites of mRNA. predominately utilized its early promoter at 24 h post-infection but by 72 h post-infection expression was almost exclusively from its late promoter. In contrast, was expressed predominantly from its early promoter throughout the timecourse, even though a consensus late promoter sequence was present within 100 bp of the translation start codon. A significant fraction of late transcripts that mapped to were spliced transcripts originating in the gene region. The 3′ termini of the transcripts were also mapped using 3′ RACE.

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2002-04-01
2019-10-14
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