1887

Abstract

Genes encoding two representatives of the LD130 family of baculovirus envelope-associated proteins were transcriptionally mapped. These included , which encodes a low pH-induced envelope fusion protein of the multinucleocapsid nucleopolyhedrovirus, and , which is related to but is encoded by MNPV and appears to lack an envelope fusion activity. The size and temporal expression of mRNA of both genes were examined by Northern blot analysis of RNA extracted from infected cells at selected timepoints. In addition, 5′ rapid amplification of cDNA ends (RACE) in combination with DNA sequence analysis was used to map the start sites of mRNA. predominately utilized its early promoter at 24 h post-infection but by 72 h post-infection expression was almost exclusively from its late promoter. In contrast, was expressed predominantly from its early promoter throughout the timecourse, even though a consensus late promoter sequence was present within 100 bp of the translation start codon. A significant fraction of late transcripts that mapped to were spliced transcripts originating in the gene region. The 3′ termini of the transcripts were also mapped using 3′ RACE.

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2002-04-01
2024-12-02
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References

  1. Ahrens C. H., Carlson C., Rohrmann G. F. 1995; Identification, sequence and transcriptional analysis of lef-3 , a gene essential for Orgyia pseudotsugata baculovirus DNA replication. Virology 210:372–382
    [Google Scholar]
  2. Ahrens C. H., Russell R., Funk C. J., Evans J. T., Harwood S. H., Rohrmann G. F. 1997; The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome. Virology 229:381–399
    [Google Scholar]
  3. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605
    [Google Scholar]
  4. Bischoff D. S., Slavicek J. M. 1996; Characterization of the Lymantria dispar nucleopolyhedrovirus 25K FP gene. Journal of General Virology 77:1913–1923
    [Google Scholar]
  5. Blissard G. W., Rohrmann G. F. 1989; Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170:537–555
    [Google Scholar]
  6. Blissard G. W., Kogan P. H., Wei R., Rohrmann G. F. 1992; A synthetic early promoter from a baculovirus: roles of the TATA box and conserved mRNA start site CAGT sequence in basal levels of transcription. Virology 190:783–793
    [Google Scholar]
  7. Chen X., IJkel W., Tarchini R., Sun X., Sandbrink H., Wang H., Peters S., Zuidema D., Lankhorst R., Vlak J., Hu Z. 2001; The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. Journal of General Virology 82:241–257
    [Google Scholar]
  8. Garrity D. B., Chang M.-J., Blissard G. W. 1997; Late promoter selection in the baculovirus gp64 envelope fusion protein gene. Virology 231:167–181
    [Google Scholar]
  9. Hashimoto Y., Hayakawa T., Ueno Y., Fugita T., Sano Y., Matsumoto T. 2000; Sequence analysis of the Plutella xylostella granulovirus genome. Virology 275:358–372
    [Google Scholar]
  10. Hayakawa T., Ko R., Okano K., Seong S., Goto C., Maeda S. 1999; Sequence analysis of the Xestia c-nigrum granulovirus genome. Virology 262:277–297
    [Google Scholar]
  11. IJkel W. F. J., van Strien E. A., Jeldens J. G. M., Broer R., Zuidema D., Goldbach R. W., Vlak J. M. 1999; Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. Journal of General Virology 80:3289–3304
    [Google Scholar]
  12. Jin J., Guarino L. A. 2000; 3′-end formation of baculovirus late RNAs. Journal of Virology 74:8930–8937
    [Google Scholar]
  13. Kuzio J., Pearson M. N., Harwood S. H., Funk C. J., Evans J. T., Slavicek J., Rohrmann G. F. 1999; Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar. Virology 253:17–34
    [Google Scholar]
  14. Lewin B. 1997 Genes VI New York: Oxford University Press;
    [Google Scholar]
  15. McCaughern-Carucci J. 1997; The ‘‘Neverfail’’ Northern blot hybridization. http://www.nwfsc.noaa.gov/protocols/northernblot.html
  16. Pang Y., Yu J., Wang L., Hu X., Bao W., Li G., Chen C., Han H., Hu S., Yang H. 2001; Sequence analysis of the Spodoptera litura multicapsid nucleopolyhedrovirus genome. Virology 287:391–404
    [Google Scholar]
  17. Pearson M. N., Groten C., Rohrmann G. F. 2000; Identification of the Lymantria dispar nucleopolyhedrovirus envelope fusion protein provides evidence for a phylogenetic division of the Baculoviridae. Journal of Virology 74:6126–6131
    [Google Scholar]
  18. Pearson M. N., Russell R., Rohrmann G. F. 2001; Characterization of a baculovirus encoded protein that is associated with infected-cell membranes and budded virions. Virology 290:22–31
    [Google Scholar]
  19. Rankin C., Ooi B. G., Miller L. K. 1988; Eight base pairs encompassing the transcriptional start point are the major determinant for baculovirus polyhedrin gene expression. Gene 70:39–49
    [Google Scholar]
  20. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  21. Slavicek J. M. 1991; Temporal analysis and spatial mapping of Lymantria dispar nuclear polyhedrosis virus transcripts and in vitro translation products. Virus Research 20:223–236
    [Google Scholar]
  22. Summers M. D., Smith G. E. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experiment Station Bulletin no. 1555
    [Google Scholar]
  23. Theilmann D. A., Stewart S. 1991; Identification and characterization of the IE-1 gene of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 180:492–508
    [Google Scholar]
  24. Volkman L. E. 1997; Nucleopolyhedrovirus interactions with their insect hosts. Advances in Virus Research 48:313–348
    [Google Scholar]
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