1887

Journal of General Virology header logo

Volume 83, Issue 4

Analysis of the complete genome of subgroup A′ hepatitis B virus isolates from South Africa Free

Abstract

A phylogenetic analysis is presented of six complete and seven pre-S1/S2/S gene sequences of hepatitis B virus (HBV) isolates from South Africa. Five of the full-length sequences and all of the pre-S2/S sequences have been previously reported. Four of the six complete genomes and three of the five incomplete sequences clustered with subgroup A′, a unique segment of genotype A of HBV previously identified in 60% of South African isolates using analysis of the pre-S2/S region alone. This separation was also evident when the polymerase open reading frame was analysed, but not on analysis of either the X or pre-core/core genes. Amino acids were identified in the pre-S1 and polymerase regions specific to subgroup A′. In common with genotype D, 10 of 11 genotype A South African isolates had an 11 amino acid deletion in the amino end of the pre-S1 region. This deletion is also found in hepadnaviruses from non-human primates.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-4-835
2002-04-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/4/0830835a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-4-835&mimeType=html&fmt=ahah

References

  1. Arauz-Ruiz P., Norder H., Visona K. A., Magnius L. O. 1997; Molecular epidemiology of hepatitis B virus in Central America reflected in the genetic variability of the small S gene. Journal of Infectious Diseases 176:851–858
     [Google Scholar]
  2. Bowyer S. M., van Staden L., Kew M. C., Sim J. G. M. 1997; A unique segment of the hepatitis B virus group A genotype identified isolates from South Africa. Journal of General Virology 78:1719–1729
     [Google Scholar]
  3. Chirara M. M., Chetsanga C. J. 1994; Variant of hepatitis B virus isolated in Zimbabwe. Journal of Medical Virology 29:73–78
     [Google Scholar]
  4. Estacio R. C., Chavez C. C., Okamoto H., Lingao A. L., Reyes M. T., Domingo E., Mayumi M. 1988; Nucleotide sequence of a hepatitis B virus genome of subtype adw isolated from a Philippino: comparison with the reported three genomes of the same subtype. Journal of Gastroenterology and Hepatology 3:215–222
     [Google Scholar]
  5. Grethe S., Heckel J.-O., Rietschel W., Hufert F. T. 2000; Molecular epidemiology of hepatitis B virus variants in nonhuman primates. Virology 74:5377–5381
     [Google Scholar]
  6. Hannoun C., Norder H., Lindh M. 2000; An aberrant genotype revealed in recombinant hepatitis B virus strains from Vietnam. Journal of General Virology 81:2262–2272
     [Google Scholar]
  7. Hu X., Margolis H. S., Purcell R. H., Ebert J., Robertson B. H. 2000; Identification of hepatitis B virus indigenous to chimpanzees. Proceedings of the National Academy of Sciences, USA 97:1661–1664
     [Google Scholar]
  8. MacDonald D. M., Holmes E. C., Lewis J. C. M., Simmonds P. 2000; Detection of hepatitis B virus infection in wild-born chimpanzees ( Pan troglodytes verus ): phylogenetic relationships with human and other primate genotypes. Journal of Virology 74:4253–4257
     [Google Scholar]
  9. Mizokami M., Orito E., Ohba K., Ikeo K., Lau J. H. N., Gojobori T. 1997; Constrained evolution with respect to gene overlap of hepatitis B virus. Journal of Molecular Evolution 44:583–590
     [Google Scholar]
  10. Moraes M. T. B., Gomes S. A., Niel C. 1996; Sequence analysis of pre-S/S gene of hepatitis B virus strains of genotypes A, D, and F isolated in Brazil. Archives of Virology 141:1767–1773
     [Google Scholar]
  11. Neurath A. R., Kent S. B. H., Strick N., Parker K. 1986; Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell 46:2305–2314
     [Google Scholar]
  12. Norder H., Hammas B., Lee S.-D., Bile K., Couroucé A.-M., Mushahwar I. K., Magnius L. O. 1993; Genetic relatedness of hepatitis B viral strains of diverse geographical origin and natural variations in the primary structure of the surface antigen. Journal of General Virology 74:1341–1348
     [Google Scholar]
  13. Norder H., Couroucé A.-M., Magnius L. O. 1994; Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 198:489–503
     [Google Scholar]
  14. Norder H., Ebert J. W., Fields H. A., Mushahwar I. K., Magnius L. O. 1996; Complete sequencing of a gibbon hepatitis B virus genome reveals a unique genotype distantly related to the chimpanzee hepatitis B virus. Virology 218:214–223
     [Google Scholar]
  15. Okamoto H., Tsuda F., Sakugawa H., Sastrosoewingnjo R. I., Imai M., Miyakawa Y., Mayumi M. 1988; Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. Journal of General Virology 69:2575–2583
     [Google Scholar]
  16. Ono Y., Onda H., Sasada R., Igarashi K., Sugino Y., Kusuya N. 1983; The complete nucleotide sequences of the cloned hepatitis B virus DNA: subtype adr and adw. Nucleic Acids Research 25:1747–1757
     [Google Scholar]
  17. Owiredu W. K. B. A., Kramvis A., Kew M. C. 2001a; Hepatitis B virus DNA in serum of healthy black African adults positive for hepatitis B surface antibody alone: possible association with recombination between genotypes A and D. Journal of Medical Virology 64:441–454
     [Google Scholar]
  18. Owiredu W. K. B. A., Kramvis A., Kew M. C. 2001b; Molecular analysis of hepatitis B virus genomes isolated from black African patients with fulminant hepatitis B. Journal of Medical Virology 65:485–492
     [Google Scholar]
  19. Pontisso P., Ruvoletto M. G., Gerlich W. H., Heermann K. H., Bardini R., Alberti A. 1989; Identification of an attachment site for human liver plasma membranes on hepatitis B virus particles. Virology 173:522–530
     [Google Scholar]
  20. Stuyver L., De Gendt S., Van Geyt C., Zoulim F., Fried M., Schinazi R. F., Rossau R. 2000; A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. Journal of General Virology 81:67–74
     [Google Scholar]
  21. Takahashi K., Brotman B., Usuda S., Mishiro S., Prince A. M. 2000; Full-genome sequence analysis of hepatitis B virus strains recovered from chimpanzees infected in the wild: implications for an origin of HBV. Virology 267:58–64
     [Google Scholar]
  22. Tran A., Kremsdorf D., Capel F., Housset C., Dauguet C., Petit M.-A., Brechot C. 1991; Emergence of and takeover by hepatitis B virus (HBV) rearrangements in the pre-S/S and pre-C/C genes during chronic HBV infection. Journal of Virology 65:3566–3574
     [Google Scholar]
  23. Uy A., Wunderlich G., Olsen D. B., Heermann K.-L., Gerlich W. H., Thomssen R. 1992; Genomic variability in the preS1 region and determination of routes of transmission of hepatitis B virus. Journal of General Virology 73:3005–3009
     [Google Scholar]
  24. Verschoor E. J., Warren K. S., Langenhuijzen S., Heriyanto Swan R. A., Heeney J. L. 2001; Analysis of two genomic variants of orangutan hepadnavirus and their relationship to other primate hepatitis B-like viruses. Journal of General Virology 82:893–897
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-4-835
Loading
Analysis of the complete genome of subgroup A′ hepatitis B virus isolates from South Africa
J. Gen. Virol. 83, 835 (2002); https://doi.org/10.1099/0022-1317-83-4-835
/content/journal/jgv/10.1099/0022-1317-83-4-835
/content/journal/jgv/10.1099/0022-1317-83-4-835
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error