1887

Abstract

Cellular receptors play an important role in virus pathogenesis. As a first step in virus infection, viruses attach to specific receptors on the surface of cells; Hantaan virus infects susceptible cells by attaching to a receptor located on the cell surface. To date, the identity of the Hantaan virus host cell receptor remains unknown. To determine the protein on the cell surface to which Hantaan virus binds, a virus overlay protein-binding assay was performed with radiolabelled virus. A 30 kDa (30K) protein was identified as a putative receptor for Hantaan virus. The specificity of virus interactions with this protein was demonstrated with a competition assay using unlabelled Hantaan virus and poliovirus. Unlabelled Hantaan virus inhibited the binding of radiolabelled Hantaan virus to this 30 kDa protein, whereas poliovirus did not. A polyclonal antibody against the 30K protein blocked binding of Hantaan virus to Vero-E6 cells and, consequently, virus infection. Blocking with the anti-30K antibody reduced virus infection of cells by 70%. These data strongly suggest that the 30 kDa surface protein is a putative receptor for Hantaan virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-4-767
2002-04-01
2020-01-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/4/0830767a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-4-767&mimeType=html&fmt=ahah

References

  1. Anderson, R., King, A. D. & Innis, B. L. ( 1992; ). Correlation of E protein binding with cell susceptibility to dengue 4 virus infection. Journal of General Virology 73, 2155-2159.[CrossRef]
    [Google Scholar]
  2. Arikawa, J., Schmaljohn, A. L., Dalrymple, J. M. & Schmaljohn, C. S. ( 1989; ). Characterization of Hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies. Journal of General Virology 70, 615-624.[CrossRef]
    [Google Scholar]
  3. Bordier, C. ( 1981; ). Phase separation of integral membrane proteins in Triton X-114 solution. Journal of Biological Chemistry 256, 1604-1607.
    [Google Scholar]
  4. Cohen, M. S. ( 1982; ). Epidemic hemorrhagic fever revisited. Reviews of Infectious Diseases 4, 992-995.[CrossRef]
    [Google Scholar]
  5. Crane, S. E., Buzy, J. & Clements, J. E. ( 1991; ). Identification of cell membrane proteins that bind visna virus. Journal of Virology 65, 6137-6143.
    [Google Scholar]
  6. Dantas, J. R.Jr, Okuno, Y., Asada, H., Tamura, M., Takahashi, M., Tanishita, O., Takahashi, Y., Kurata, T. & Yamanishi, K. ( 1986; ). Characterization of glycoproteins of viruses causing hemorrhagic fever with renal syndrome (HFRS) using monoclonal antibodies. Virology 151, 379-384.[CrossRef]
    [Google Scholar]
  7. Daughaday, C. C., Brandt, W. E., McCown, J. M. & Russell, P. K. ( 1981; ). Evidence for two mechanisms of dengue virus infection of adherent human monocytes: trypsin-sensitive virus receptors and trypsin-resistant immune complex receptors. Infection and Immunity 32, 469-473.
    [Google Scholar]
  8. Elliott, L. H., Kiley, M. P. & McCormick, J. B. ( 1984; ). Hantaan virus: identification of virion proteins. Journal of General Virology 65, 1285-1293.[CrossRef]
    [Google Scholar]
  9. Fields, B. N. & Greene, M. I. ( 1982; ). Genetic and molecular mechanisms of viral pathogenesis: implications for prevention and treatment. Nature 300, 19-23.[CrossRef]
    [Google Scholar]
  10. Gavrilovskaya, I. N., Shepley, M., Shaw, R., Ginsberg, M. H. & Mackow, E. R. ( 1998; ). β3 Integrins mediate the cellular entry of hantaviruses that cause respiratory failure. Proceedings of the National Academy of Sciences, USA 95, 7074-7079.[CrossRef]
    [Google Scholar]
  11. Gavrilovskaya, I. N., Brown, E. J., Ginsberg, M. H. & Mackow, E. R. ( 1999; ). Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by β3 integrins. Journal of Virology 73, 3951-3959.
    [Google Scholar]
  12. Gonzalez, S. F., Shope, R. E., Calisher, C. E. & Nathanson, N. ( 1982; ). Characterization of monoclonal antibodies against the G1 and N proteins of La Crosse and Tahyna, two California serogroup bunyaviruses. Virology 120, 42-53.[CrossRef]
    [Google Scholar]
  13. Grady, L. J., Srihongse, S., Grayson, M. A. & Deibel, R. ( 1983; ). Monoclonal antibodies against La Crosse virus. Journal of General Virology 64, 1699-1704.[CrossRef]
    [Google Scholar]
  14. Ito, Y., Kurogi, H., Takahshi, H., Goto, Y., Inaba, Y. & Omori, T. ( 1979; ). Electron microscopy of Akabane virus. Acta Virologica 23, 198-202.
    [Google Scholar]
  15. Jin, Y. M., Pardoe, I. U., Burness, A. T. H. & Michalak, T. I. ( 1994; ). Identification and characterization of the cell surface 70-kilodalton sialoglycoprotein(s) as a candidate receptor for encephalomyocarditis virus on human nucleated cells. Journal of Virology 68, 7308-7319.
    [Google Scholar]
  16. Kariwa, H., Arikawa, J., Takashima, I., Isegawa, Y., Yamanishi, K. & Hashimoto, N. ( 1994; ). Enhancement of infectivity of hantavirus in cell culture by centrifugation. Journal of Virological Methods 49, 235-244.[CrossRef]
    [Google Scholar]
  17. Kingford, L., Ishizawa, L. D. & Hill, D. W. ( 1983; ). Biological activities of monoclonal antibodies reactive with antigenic sites mapped on the G1 glycoprotein of La Crosse virus. Virology 90, 443-445.
    [Google Scholar]
  18. Lee, H. W. ( 1982; ). Korean hemorrhagic fever. Progress in Medical Virology 28, 96-113.
    [Google Scholar]
  19. Lee, H. W., French, G. R., Lee, P. W., Baek, L. J., Tsuchiya, K. & Foulke, R. S. ( 1981; ). Observation on natural and laboratory infection of rodents with the etiologic agent of Korean hemorrhagic fever. American Journal of Tropical Medicine and Hygiene 30, 477-482.
    [Google Scholar]
  20. Ludwig, G. V., Kondig, J. P. & Smith, J. F. ( 1996; ). A putative receptor for Venezuelan equine encephalitis virus from mosquito cells. Journal of Virology 70, 5592-5599.
    [Google Scholar]
  21. Marianneau, P., Mégret, F., Olivier, R., Morens, D. M. & Deubel, V. ( 1996; ). Dengue 1 virus binding to human hepatoma HepG2 and simian Vero cell surfaces differs. Journal of General Virology 77, 2547-2554.[CrossRef]
    [Google Scholar]
  22. Martin, M., Lindsey-Regnery, H., Sasso, D. R., McCormick, J. B. & Palmer, E. ( 1985; ). Distinction between Bunyaviridae genera by surface structure and comparison with Hantaan virus using negative stain electron microscopy. Archives of Virology 86, 17-28.[CrossRef]
    [Google Scholar]
  23. Salas-Benito, J. S. & del Angel, R. M. ( 1997; ). Identification of two surface proteins from C6/36 cells that bind dengue type 4 virus. Journal of Virology 71, 7246-7252.
    [Google Scholar]
  24. Smith, G. W. & Wright, P. J. ( 1985; ). Synthesis of proteins and glycoproteins in Dengue type 2 virus-infected Vero and Aedes albopictus cells. Journal of General Virology 66, 559-571.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-4-767
Loading
/content/journal/jgv/10.1099/0022-1317-83-4-767
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error