1887

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Volume 83, Issue 3

Factors determining vector competence and specificity for transmission of Free

Abstract

The competence of a and a population to transmit (TSWV) was analysed. Adults of the population transmitted this virus efficiently, whereas those of the thelytokous population failed to transmit. TSWV replicated in the midgut of the larvae of both populations after ingestion of virus; however, lower amounts accumulated in larvae than in larvae. The virus was almost undetectable in adults, whereas high titres were readily detected in the adults. The first infections in larvae were detected by immunocytochemical studies in midgut epithelial and subsequently in midgut muscle cells, the ligaments, and finally in the salivary glands. The infections were weaker in the midgut epithelial and muscle cells of larvae, followed by an almost complete absence of any infection in the ligaments, and a complete absence in the salivary glands. Studies by electron microscopy revealed the budding of some virus particles from the basal membrane of midgut epithelial cells of larvae into the extracellular space of the basal labyrinth. Enveloped virus particles were also seen in midgut muscle cells of larvae. They were not discerned in epithelial and muscle cells of larvae and adults. This study showed that the rate of virus replication in the midgut and the extent of virus migration from the midgut to the visceral muscle cells and the salivary glands are probably crucial factors in the determination of vector competence.

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2002-03-01
2024-04-18
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Factors determining vector competence and specificity for transmission of Tomato spotted wilt virus
J. Gen. Virol. 83, 663 (2002); https://doi.org/10.1099/0022-1317-83-3-663
/content/journal/jgv/10.1099/0022-1317-83-3-663
/content/journal/jgv/10.1099/0022-1317-83-3-663
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