RT Journal Article SR Electronic(1) A1 Tsitoura, Eliza A1 Lucas, Michaela A1 Revol-Guyot, Valerie A1 Epstein, Alberto L. A1 Manservigi, Roberto A1 Mavromara, PenelopeYR 2002 T1 Expression of hepatitis C virus envelope glycoproteins by herpes simplex virus type 1-based amplicon vectors JF Journal of General Virology, VO 83 IS 3 SP 561 OP 566 DO https://doi.org/10.1099/0022-1317-83-3-561 PB Microbiology Society, SN 1465-2099, AB Herpes simplex virus type 1 (HSV-1)-based amplicon vectors expressing hepatitis C virus (HCV) E1 and E2 glycoproteins were investigated. HSV-1 amplicon vectors carrying the E1E2p7- or E2p7-coding sequences of HCV type 1a under the control of the HSV-1 IE4 (α22/α47) promoter were constructed. Studies of infected HepG2, WRL 68 or Vero cells indicated that HSV-1-based amplicon vectors express high levels of HCV glycoproteins that are processed correctly. Immunofluorescence microscopy combined with immunoprecipitation and endoglycosidase treatment of cells infected with the HSV-1-based vectors expressing E1 and E2 showed that the two glycoproteins were retained in the endoplasmic reticulum and had the expected glycosylation patterns. Furthermore, although most of the E1 and E2 proteins formed disulfide-linked aggregates, significant amounts of monomeric forms of the two proteins were detected by SDS–PAGE under non-reducing conditions, suggesting the presence of non-covalently associated E1 and E2. Similar results were produced by a replication-competent recombinant HSV-1 vector expressing HCV E1 and E2. These results indicated that HSV-1-based amplicon vectors represent a useful expression system for the study of HCV glycoproteins., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-83-3-561