RT Journal Article SR Electronic(1) A1 Armengol, Elisenda A1 Wiesmüller, Karl-Heinz A1 Wienhold, Daniel A1 Büttner, Mathias A1 Pfaff, Eberhard A1 Jung, Günther A1 Saalmüller, ArminYR 2002 T1 Identification of T-cell epitopes in the structural and non-structural proteins of classical swine fever virus JF Journal of General Virology, VO 83 IS 3 SP 551 OP 560 DO https://doi.org/10.1099/0022-1317-83-3-551 PB Microbiology Society, SN 1465-2099, AB To identify new T-cell epitopes of classical swine fever virus (CSFV), 573 overlapping, synthetic pentadecapeptides spanning 82% of the CSFV (strain Glentorf) genome sequence were synthesized and screened. In proliferation assays, 26 peptides distributed throughout the CSFV viral protein sequences were able to induce specific T-cell responses in PBMCs from a CSFV-Glentorf-infected d/d haplotype pig. Of these 26 peptides, 18 were also recognized by PBMCs from a CSFV-Alfort/187-infected d/d haplotype pig. In further experiments, it could be shown that peptide 290 (KHKVRNEVMVHWFDD), which corresponds to amino acid residues 1446–1460 of the CSFV non-structural protein NS2–3 could induce interferon-γ secretion after secondary in vitro restimulation. The major histocompatibility complex (MHC) restriction for stimulation of T-cells by this pentadecapeptide was identified as being mainly MHC class II and partially MHC class I. In cytolytic assays, CSFV-specific cytotoxic T-lymphocytes (CTLs) were able to lyse peptide 290-loaded target cells. These findings indicate the existence of a CSFV-specific helper T-cell epitope and a CTL epitope in this peptide., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-83-3-551