1887

Abstract

Human rhinoviruses (HRV), common agents of respiratory infections, comprise 102 designated serotypes. The genetic relationships of HRV prototype strains and the possibility of using genetic identification of a given HRV field strain were studied. Genomic sequences in the VP4/VP2 region were obtained from all 102 prototype strains. Phylogenetic analysis included 61 recently isolated Finnish field strains. Seventy-six out of the 102 prototype strains clustered in the HRV genetic group A and 25 in group B. Serotype 87 clustered separately and together with human enterovirus 70. The ‘percentage’ interserotypic differences were generally similar to those between different enterovirus serotypes, but for six pairs of HRV serotypes they were less than 10%. The maximum variation in genetic group A was 41% at the nucleotide level and 28% at the amino acid level, and in genetic group B 34% and 20%, respectively. Judging from the observed interserotypic differences, the 61 Finnish field isolates might represent as many as 19 different serotypes. One cluster of the field strains did not directly associate with any of the prototype strains and might represent a new serotype. However, larger numbers of field isolates of known serotype need to be characterized, possibly also in the VP1 region, to evaluate the feasibility of genetic typing of HRV strains.

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2002-02-01
2019-10-19
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References

  1. Abraham, G. & Colonno, R. J. ( 1984; ). Many rhinovirus serotypes share the same cellular receptor. Journal of Virology 51, 340-345.
    [Google Scholar]
  2. Abzug, M. J., Beam, A. C., Gyorkos, E. A. & Levin, M. J. ( 1990; ). Viral pneumonia in the first month of life. Pediatric Infectious Disease Journal 9, 881-885.[CrossRef]
    [Google Scholar]
  3. Andeweg, A. C., Bestebroer, T. M., Huybreghs, M., Kimman, T. G. & de Jong, J. C. ( 1999; ). Improved detection of rhinoviruses in clinical samples by using a newly developed nested reverse transcription–PCR assay. Journal of Clinical Microbiology 37, 524-530.
    [Google Scholar]
  4. Andries, K., Dewindt, B., Snoekes, J., Wouters, L., Moereels, H., Lewi, P. J. & Janssen, P. A. J. ( 1990; ). Two groups of rhinoviruses revealed by a panel of antiviral compounds present sequence divergence and differential pathogenicity. Journal of Virology 64, 1117-1123.
    [Google Scholar]
  5. Arola, M., Ziegler, T., Ruuskanen, O., Mertsola, J., Nanto-Salonen, K. & Halonen, P. ( 1988; ). Rhinoviruses in acute otitis media. Journal of Pediatrics 113, 693-695.[CrossRef]
    [Google Scholar]
  6. Arruda, E. & Hayden, F. G. ( 1993; ). Detection of human rhinovirus RNA in nasal washings by PCR. Molecular and Cellular Probes 7, 373-379.[CrossRef]
    [Google Scholar]
  7. Blomqvist, S., Skyttä, A., Roivainen, M. & Hovi, T. ( 1999; ). Rapid detection of human rhinoviruses in nasopharyngeal aspirates by a microwell reverse transcription–PCR-hybridization assay. Journal of Clinical Microbiology 37, 2813-2816.
    [Google Scholar]
  8. Cooney, M. K., Fox, J. P. & Kenny, G. E. ( 1982; ). Antigenic groupings of 90 rhinovirus serotypes. Infection and Immunology 37, 642-647.
    [Google Scholar]
  9. Couch, R. B. ( 1992; ). Rhinoviruses. In Laboratory Diagnosis of Viral Infections , pp. 709-729. Edited by E. H. Lennette. New York:Marcel Dekker.
  10. Duechler, M., Skern, T., Sommergruber, W., Neubauer, C., Gruendler, P., Fogy, I., Blaas, D. & Kuechler, E. ( 1987; ). Evolutionary relationships within the human rhinovirus genus: comparison of serotypes 89, 2 and 14. Proceedings of the National Academy of Sciences, USA 84, 2605-2609.[CrossRef]
    [Google Scholar]
  11. Felsenstein, J. (1993). PHYLIP (Phylogeny Inference package) version 3.5c. Distributed by the author, Department of Genetics, University of Washington, Seattle, USA.
  12. Gama, R. E., Horsnell, P. R., Hughes, P. J., North, C., Bruce, C. B., al-Nakib, W. & Stanway, G. ( 1989; ). Amplification of rhinovirus specific nucleic acids from clinical samples using the polymerase chain reaction. Journal of Medical Virology 28, 73-77.[CrossRef]
    [Google Scholar]
  13. Halonen, P., Rocha, E., Hierholzer, J., Holloway, B., Hyypiä, T., Hurskainen, P. & Pallansch, M. A. ( 1995; ). Detection of enteroviruses and rhinoviruses in clinical specimens by PCR and liquid-phase hybridization. Journal of Clinical Microbiology 33, 648-653.
    [Google Scholar]
  14. Hewat, E. A., Neumann, E., Conway, J. F., Moser, R., Ronacher, B., Marlovits, T. C. & Blaas, D. ( 2000; ). The cellular receptor to human rhinovirus 2 binds around the 5-fold axis and not in the canyon: a structural view. EMBO Journal 19, 6317-6325.[CrossRef]
    [Google Scholar]
  15. Horsnell, C., Gama, R. E., Hughes, P. J. & Stanway, G. ( 1995; ). Molecular relationships between 21 human rhinovirus serotypes. Journal of General Virology 76, 2549-2555.[CrossRef]
    [Google Scholar]
  16. Hughes, P. J., North, C., Jellis, C. H., Minor, P. D. & Stanway, G. ( 1988; ). The nucleotide sequence of human rhinovirus 1B: molecular relationships within the rhinovirus genus. Journal of General Virology 69, 49-58.[CrossRef]
    [Google Scholar]
  17. Hyypiä, T., Auvinen, P. & Maaronen, M. ( 1989; ). Polymerase chain reaction for human picornaviruses. Journal of General Virology 70, 3261-3268.[CrossRef]
    [Google Scholar]
  18. Ireland, D. C., Kent, J. & Nicholson, K. G. ( 1993; ). Improved detection of rhinoviruses in nasal and throat swabs by seminested RT–PCR. Journal of Medical Virology 40, 96-101.[CrossRef]
    [Google Scholar]
  19. Johnston, S. L., Sanderson, G., Pattemore, P. K., Smith, S., Bardin, P. G., Bruce, C. B., Lambden, P. R., Tyrrell, D. A. J. & Holgate, S. T. ( 1993; ). Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms. Journal of Clinical Microbiology 31, 111-117.
    [Google Scholar]
  20. Johnston, S. L., Pattemore, P. K., Sanderson, G., Smith, S., Lampe, F., Josephs, L., Symington, P., O’Toole, S., Myint, S. H., Tyrrell, D. A. J. & Holgate, S. T. ( 1995; ). Community study of role of virus infections in exacerbations of asthma in 9–11 year old children. British Medical Journal 310, 1225-1229.[CrossRef]
    [Google Scholar]
  21. Kew, O. M., Mulders, M. N., Lipskaya, G. Y., da Silva, E. E. & Pallansch, M. A. ( 1995; ). Molecular epidemiology of enteroviruses. Seminars in Virology 6, 401-414.[CrossRef]
    [Google Scholar]
  22. Mäkelä, M. J., Puhakka, T., Ruuskanen, O., Leinonen, M., Saikku, P., Kimpimäki, M., Blomqvist, S., Hyypiä, T. & Arstila, P. ( 1998; ). Viruses and bacteria in the etiology of the common cold. Journal of Clinical Microbiology 36, 539-542.
    [Google Scholar]
  23. Mulders, M. N., Salminen, M., Kalkkinen, N. & Hovi, T. ( 2000; ). Molecular epidemiology of coxsackievirus B4 and disclosure of the correct VP1/2Apro cleavage site: evidence for high genomic diversity and long-term endemicity of distinct genotypes. Journal of General Virology 81, 803-812.
    [Google Scholar]
  24. Oberste, M. S., Maher, K., Kilpatrick, D. R. & Pallansch, M. A. ( 1999a; ). Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. Journal of Virology 73, 1941-1948.
    [Google Scholar]
  25. Oberste, M. S., Maher, K., Kilpatrick, D. R., Flemister, M. R., Brown, B. A. & Pallansch, M. A. ( 1999b; ). Typing of human enteroviruses by partial sequencing of VP1. Journal of Clinical Microbiology 37, 1288-1293.
    [Google Scholar]
  26. Rueckert, R. R. ( 1996; ). Picornaviridae: the viruses and their replication. In Fields Virology , pp. 609-654. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia:Lippincott–Raven.
  27. Samuelson, A., Westmoreland, D., Eccles, R. & Fox, J. D. ( 1998; ). Development and application of a new method for amplification and detection of human rhinovirus RNA. Journal of Virological Methods 71, 197-209.[CrossRef]
    [Google Scholar]
  28. Savolainen, C., Mulders, M. N. & Hovi, T. (2001). Phylogenetic analysis of rhinovirus isolates collected during successive epidemic seasons. Virus Research (in press).
  29. Uncapher, C. R., Dewitt, C. M. & Colonno, R. J. ( 1991; ). The major and minor group receptor families contain all but one human rhinovirus serotype. Virology 180, 814-817.[CrossRef]
    [Google Scholar]
  30. Utagawa, E. T., Miyamura, K., Mukoyama, A. & Kono, R. ( 1982; ). Neuraminidase-sensitive erythrocyte receptor for enterovirus type 70. Journal of General Virology 63, 141-148.[CrossRef]
    [Google Scholar]
  31. Vesa, S., Kleemola, M., Blomqvist, S., Takala, A., Kilpi, T. & Hovi, T. ( 2001; ). Epidemiology of documented viral respiratory infections and acute otitis media in a cohort of children followed from two to twenty-four months of age. Pediatric Infectious Disease Journal 20, 574-581.[CrossRef]
    [Google Scholar]
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