1887

Abstract

Hairy leukoplakia (HL), an epithelial lesion found on the side of the tongue in immunocompromised individuals, is characterized by high-level replication of Epstein–Barr virus (EBV) and multiple EBV strains. The source of these strains and their relationship to peripheral blood lymphocyte (PBL) strains has not previously been characterized. Using matched pairs of HL scrapings and PBL from 16 HIV-positive men, variation in EBV strain identity was characterized by detection of a 30 nucleotide deletion of the EBV latent membrane protein (LMP)-1 gene, variation in the LMP-1 repeat region and typing for Epstein–Barr nuclear antigen (EBNA)-2. Multiple EBV strains were found in both the HL and PBL specimens, but 13 of 16 (81%) patients showed evidence of strain identity for at least one strain and analysis of two patients suggested that EBV strains from HL could infect the PBL. Our data are consistent with active trafficking of EBV between these two compartments.

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2002-02-01
2019-10-14
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References

  1. Allday, M. J. & Crawford, D. H. (1988). Role of epithelium in EBV persistence and pathogenesis of B-cell tumours. Lancet i, 855–857.
  2. Baer, R., Bankier, A. T., Biggin, M. D., Deininger, P. L., Farrell, P. J., Gibson, T. J., Hatfull, G., Hudson, G. S., Satchwell, S. C., Seguin, C. and others ( 1984; ). DNA sequence and expression of the B95-8 Epstein–Barr virus genome. Nature 310, 207–211.[CrossRef]
    [Google Scholar]
  3. Brandwein, M., Nuovo, G., Ramer, M., Orlowski, W. & Miller, L. ( 1996; ). Epstein–Barr virus reactivation in hairy leukoplakia. Modern Pathology 9, 298-303.
    [Google Scholar]
  4. Gratama, J. W., Oosterveer, M. A., Zwaan, F. E., Lepoutre, J., Klein, G. & Ernberg, I. ( 1988; ). Eradication of Epstein–Barr virus by allogeneic bone marrow transplantation: implications for sites of viral latency. Proceedings of the National Academy of Sciences, USA 85, 8693-8696.[CrossRef]
    [Google Scholar]
  5. Greenspan, J. S., Greenspan, D., Lennette, E. T., Abrams, D. I., Conant, M. A., Petersen, V. & Freese, U. K. ( 1985; ). Replication of Epstein–Barr virus within the epithelial cells of oral ‘hairy’ leukoplakia, an AIDS-associated lesion. New England Journal of Medicine 313, 1564-1571.[CrossRef]
    [Google Scholar]
  6. Hu, L.-F., Zabarovsky, E. R., Chen, F., Cao, S.-L., Ernberg, I., Klein, G. & Winberg, G. ( 1991; ). Isolation and sequencing of the Epstein–Barr virus BNLF-1 gene (LMP1) from a nasopharyngeal carcinoma. Journal of General Virology 72, 2399-2409.[CrossRef]
    [Google Scholar]
  7. Knecht, H., Bachmann, E., Brousset, P., Sandvej, K., Nadal, D., Bachmann, F., Odermatt, B. F., Delsol, G. & Pallesen, G. ( 1993; ). Deletions within the LMP1 oncogene of Epstein–Barr virus are clustered in Hodgkin’s disease and identical to those observed in nasopharyngeal carcinoma. Blood 82, 2937-2942.
    [Google Scholar]
  8. Miller, W. E., Edwards, R. H., Walling, D. M. & Raab-Traub, N. ( 1994; ). Sequence variation in the Epstein–Barr virus latent membrane protein 1. Journal of General Virology 75, 2729-2740.[CrossRef]
    [Google Scholar]
  9. Niedobitek, G., Agathanggelou, A., Steven, N. & Young, L. S. ( 2000; ). Epstein–Barr virus (EBV) in infectious mononucleosis: detection of the virus in tonsillar B lymphocytes but not in desquamated oropharyngeal epithelial cells. Journal of Clinical Pathology: Molecular Pathology 53, 37-42.[CrossRef]
    [Google Scholar]
  10. Palefsky, J. M., Berline, J., Penaranda, M. E., Lennette, E. T., Greenspan, D. & Greenspan, J. S. ( 1996; ). Sequence variation of latent membrane protein-1 of Epstein–Barr virus strains associated with hairy leukoplakia. Journal of Infectious Diseases 173, 710-714.[CrossRef]
    [Google Scholar]
  11. Rickinson, A. B. ( 1990; ). On the Biology of Epstein–Barr Virus Persistence: A Reappraisal. In Immunobiology and Prophylaxis of Human Herpes Virus Infections , pp. 137-146. Edited by C. Lopez. New York:Plenum Press.
  12. Sixbey, J. W., Nedrud, J. G., Raab-Traub, N., Hanes, R. A. & Pagano, J. S. ( 1984; ). Epstein–Barr virus replication in oropharyngeal epithelial cells. New England Journal of Medicine 310, 1225-1230.[CrossRef]
    [Google Scholar]
  13. Sixbey, J. W., Shirley, P., Chesney, P. J., Buntin, D. M. & Resnick, L. (1989). Detection of a second widespread strain of Epstein–Barr virus. Lancet ii, 761–765.
  14. Walling, D. M. & Raab-Traub, N. ( 1994; ). Epstein–Barr virus intrastrain recombination in oral hairy leukoplakia. Journal of Virology 68, 7909-7917.
    [Google Scholar]
  15. Walling, D. M., Edmiston, S. N., Sixbey, J. W., Abdel-Hamid, M., Resnick, L. & Raab-Traub, N. ( 1992; ). Coinfection with multiple strains of the Epstein–Barr virus in human immunodeficiency virus-associated hairy leukoplakia. Proceedings of the National Academy of Sciences, USA 89, 6560-6564.[CrossRef]
    [Google Scholar]
  16. Walling, D. M., Clark, N. M., Markowitz, D. M., Frank, T. S., Braun, D. K., Eisenberg, E., Krutchkoff, D. J., Felix, D. H. & Raab-Traub, N. ( 1995; ). Epstein–Barr virus coinfection and recombination in non-human immunodeficiency virus-associated oral hairy leukoplakia. Journal of Infectious Diseases 171, 1122-1130.[CrossRef]
    [Google Scholar]
  17. Yao, Q. Y., Rowe, M., Martin, B., Young, L. S. & Rickinson, A. B. ( 1991; ). The Epstein–Barr virus carrier state: dominance of a single growth-transforming isolate in the blood and in the oropharynx of healthy virus carriers. Journal of General Virology 72, 1579-1590.[CrossRef]
    [Google Scholar]
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