1887

Abstract

Poliovirus isolates were screened for recombinants by combined analysis of two distant polymorphic segments of the poliovirus genome (one in the capsid and the other in the polymerase-coding region). Using a restriction fragment length polymorphism (RFLP) assay, a high number of recombinant genomes was found among vaccine-derived strains excreted by poliovirus vaccine vaccinees or vaccine-associated paralytic poliomyelitis cases. Some of these subjects carried a wild-type poliovirus (non-vaccine-specific) nucleotide sequence in the 3′ part of the genome. Using a similar approach, a collection of wild-type poliovirus strains isolated in South India between 1985 and 1993 was screened for recombinants. Genotypes were defined by the parallel application of RFLP assays and genomic sequencing of the capsid protein VP1 and the 3D polymerase polypeptide. Analyses revealed several instances where the position of an isolate on the phylogenic tree for the capsid protein-coding segment did not agree with its position on the tree for the polymerase-coding region. In this way, several wild-type/wild-type and wild-type/vaccine recombinants could be identified, indicating that recombination is encountered commonly in the natural evolution of poliovirus strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-12-3103
2002-12-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/12/0833103a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-12-3103&mimeType=html&fmt=ahah

References

  1. Balanant, J., Guillot, S., Candrea, A., Delpeyroux, F. & Crainic, R. ( 1991; ). The natural genomic variability of poliovirus analyzed by a restriction fragment length polymorphism assay. Virology 184, 645-654.[CrossRef]
    [Google Scholar]
  2. Burke, D. S. ( 1997; ). Recombination in HIV: an important viral evolutionary strategy. Emerging Infectious Diseases 3, 253-259.[CrossRef]
    [Google Scholar]
  3. Chenault, K. D. & Melcher, U. ( 1994; ). Phylogenic relationships reveal recombination among isolates of cauliflower mosaic virus. Journal of Molecular Evolution 39, 496-505.
    [Google Scholar]
  4. Cooper, P. D. ( 1977; ). Genetics of polioviruses. Comprehensive Virology 9, 133-207.
    [Google Scholar]
  5. Felsenstein, J. (1993). phylip: Phylogeny Inference Package, version 3.5C. University of Washington, Seattle, WA, USA.
  6. Furione, M., Guillot, S., Otelea, D., Balanant, J., Candrea, A. & Crainic, R. ( 1993; ). Polioviruses with natural recombinant genomes isolated from vaccine-associated paralytic poliomyelitis. Virology 196, 199-208.[CrossRef]
    [Google Scholar]
  7. Georgescu, M. M., Delpeyroux, F., Tardy-Panit, M., Balanant, J., Combiescu, M., Combiescu, A. A., Guillot, S. & Crainic, R. ( 1994; ). High diversity of poliovirus strains isolated from the central nervous system from patients with vaccine-associated paralytic poliomyelitis. Journal of Virology 68, 8089-8101.
    [Google Scholar]
  8. Gibson, T., Higgins, D. & Thompson, J. (1994). clustalw, version 1.7. EMBL, Heidelberg, Germany.
  9. Goldbach, R. ( 1986; ). Molecular evolution of plant RNA viruses. Annual Review of Phytopathology 24, 289-310.[CrossRef]
    [Google Scholar]
  10. Gromeier, M., Alexander, L. & Wimmer, E. ( 1996; ). Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proceedings of the National Academy of Sciences, USA 93, 2370-2375.[CrossRef]
    [Google Scholar]
  11. Guillot, S., Caro, V., Cuervo, N., Korotkova, E., Combiescu, M., Persu, A., Aubert-Combiescu, A., Delpeyroux, F. & Crainic, R. ( 2000; ). Natural genetic exchanges between vaccine and wild poliovirus strains in humans. Journal of Virology 74, 8434-8443.[CrossRef]
    [Google Scholar]
  12. Holland, J. J., Spindler, K., Horodyski, F., Grabau, E., Nichol, S. & VandePol, S. ( 1982; ). Rapid evolution of RNA genomes. Science 215, 1577-1585.[CrossRef]
    [Google Scholar]
  13. John, T. J. ( 1984; ). Poliomyelitis in India: prospects and problems of control. Reviews of Infectious Diseases 6, S438-S441.[CrossRef]
    [Google Scholar]
  14. John, T. J. & Ratnam, P. V. ( 1969; ). Comparative sensitivity of two techniques of specimen inoculation for the isolation of virus. Indian Journal of Medical Research 57, 1841-1845.
    [Google Scholar]
  15. Kew, O. M., Mulders, M. N., Lipskaya, G. Y., da Silva, E. & Pallansch, M. ( 1995; ). Molecular epidemiology of polioviruses. Seminars in Virology 6, 401-414.[CrossRef]
    [Google Scholar]
  16. King, A. M. Q., McCahon, D., Slade, W. R. & Newman, J. W. I. ( 1982; ). Recombination in RNA. Cell 29, 921-928.[CrossRef]
    [Google Scholar]
  17. Kirkegaard, K. & Baltimore, D. ( 1986; ). The mechanism of RNA recombination in poliovirus. Cell 47, 433-443.[CrossRef]
    [Google Scholar]
  18. Lai, M. M. ( 1992; ). Genetic recombination in RNA viruses. Current Topics in Microbiology and Immunology 176, 21-32.
    [Google Scholar]
  19. Lipskaya, G. Y., Muzychenko, A. R., Kutitova, O. K., Maslova, S. V., Equestre, M., Drozdov, S. G., Bercoff, R. P. & Agol, V. I. ( 1991; ). Frequent isolation of intertypic poliovirus recombinants with serotype 2 specificity from vaccine-associated polio cases. Journal of Medical Virology 35, 290-296.[CrossRef]
    [Google Scholar]
  20. Melnick, J. L. ( 1990; ). Enteroviruses. In Virology , pp. 549-609. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia:Lippincott–Raven.
  21. Melnick, J., Wenner, H. A. & Phillips, C. A. (1979). Enteroviruses. In Diagnostic Procedures of Viral, Rickettsial and Chlamydial Infections, pp. 471–534. Edited by E. H. Lennette & N. J. Schmidt. American Public Health Association.
  22. Minor, P. D., John, A., Ferguson, M. & Icenogle, J. P. ( 1986; ). Antigenic and molecular evolution of the vaccine strain of type 3 poliovirus during the period of excretion by a primary vaccinee. Journal of General Virology 67, 693-706.[CrossRef]
    [Google Scholar]
  23. Page, R. D. ( 1996; ). treeview: an application to display phylogenic trees on personal computers. Computer Applications in the Biosciences 12, 357-358.
    [Google Scholar]
  24. Poyry, T., Kinnunen, L. & Hovi, T. ( 1992; ). Genetic variation in vivo and proposed functional domains of the 5′ noncoding region of poliovirus RNA. Journal of Virology 66, 5313-5319.
    [Google Scholar]
  25. Revers, F., Le Gall, O., Candresse, T., Le Romancer, M. & Dunez, J. ( 1996; ). Frequent occurrence of recombinant potyvirus isolates. Journal of General Virology 77, 1953-1965.[CrossRef]
    [Google Scholar]
  26. Rico-Hesse, R., Pallansch, M. A., Nottay, B. K. & Kew, O. M. ( 1987; ). Geographic distribution of wild-type poliovirus type 1 genotypes. Virology 160, 311-322.[CrossRef]
    [Google Scholar]
  27. Sawyer, S. ( 1989; ). Statistical tests for detecting gene conversion. Molecular Biology and Evolution 6, 526-538.
    [Google Scholar]
  28. Tang, R. S., Barton, D. J., Flanegan, J. B. & Kirkegaard, K. ( 1997; ). Poliovirus RNA recombination in cell-free extracts. RNA 3, 624-633.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-12-3103
Loading
/content/journal/jgv/10.1099/0022-1317-83-12-3103
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error