1887

Abstract

Epstein–Barr virus (EBV) persistently infects B-cells in humans and can be shed into the saliva. The cellular source of infectious virus is uncertain. Hairy leukoplakia, an AIDS-associated lesion of the tongue, supports EBV replication in epithelial cells. However, the general significance of this observation has remained doubtful. Using immunohistochemistry and hybridization, we demonstrate evidence of EBV replication in tongue epithelial cells in 4 of 168 samples from 84 autopsy cases. Thus, in patients who do not have AIDS, squamous epithelial cells of the tongue rarely support EBV replication. However, all individuals with evidence of EBV replication were either on immunosuppressive therapy or were terminally ill cancer patients, suggesting that an impairment of the immune system may have allowed EBV replication to occur at this site. Thus, our findings are consistent with the idea that EBV replication in oropharyngeal epithelial cells is an infrequent event.

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2002-12-01
2020-01-21
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References

  1. Allday, M. J. & Crawford, D. H. (1988). Role of epithelium in EBV persistence and pathogenesis of B-cell tumours. Lancet i, 855–857.
  2. Crawford, D. H. & Edwards, J. M. B. ( 1987; ). Epstein–Barr virus. In Principles and Practice of Clinical Virology , pp. 111-133. Edited by A. J. Zuckerman, J. E. Banatvala & J. R. Pattison. Chichester:John Wiley.
  3. Gerdes, J., Schwab, U., Lemke, H. & Stein, H. ( 1983; ). Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. International Journal of Cancer 31, 13-20.[CrossRef]
    [Google Scholar]
  4. Greenspan, J. S., Greenspan, D., Lennette, E. T., Abrams, D. I., Canant, M. A., Petersen, V. & Freese, U. K. ( 1985; ). Replication of Epstein–Barr virus within the epithelial cells of oral hairy leukoplakia, an AIDS-associated lesion. New England Journal of Medicine 313, 1564-1571.[CrossRef]
    [Google Scholar]
  5. Hessing, M., van Schijndel, H. B., van Grunsven, W. M., Wolf, H. & Middeldorp, J. M. ( 1992; ). Purification and quantification of recombinant Epstein–Barr viral glycoproteins gp350/220 from Chinese hamster ovary cells. Journal of Chromatography 599, 267-272.[CrossRef]
    [Google Scholar]
  6. International Agency for Research on Cancer (1997). Epstein–Barr virus and Kaposi’s sarcoma herpesvirus/human herpesvirus-8. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans (vol. 70). Lyon, France: WHO.
  7. Karajannis, M. A., Hummel, M., Anagnostopoulos, I. & Stein, H. ( 1997; ). Strict lymphotropism of Epstein–Barr virus during acute infectious mononucleosis in non-immunocompromised individuals. Blood 89, 2856-2862.
    [Google Scholar]
  8. Lemon, S. M., Hutt, L. M., Shaw, J. E., Li, J.-L. H. & Pagano, J. S. ( 1977; ). Replication of EBV in epithelial cells during infectious mononucleosis. Nature 70, 268-270.
    [Google Scholar]
  9. Miller, G. ( 1990; ). The switch between latency and replication of Epstein–Barr virus. Journal of Infectious Diseases 161, 833-844.[CrossRef]
    [Google Scholar]
  10. Miller, G. & Lipman, M. ( 1973; ). Release of infectious Epstein–Barr virus by transformed marmoset leukocytes. Proceedings of the National Academy of Sciences, USA 70, 190-194.[CrossRef]
    [Google Scholar]
  11. Niedobitek, G. & Young, L. S. ( 1994; ). Epstein–Barr virus persistence and virus-associated tumours. Lancet 343, 333-335.[CrossRef]
    [Google Scholar]
  12. Niedobitek, G. & Herbst, H. ( 2001; ). In-situ detection of Epstein–Barr virus DNA and of viral gene products. In Epstein–Barr Virus Protocols , pp. 79-91. Edited by J. B. Wilson & G. H. W. May. Totowa, NJ:Humana Press.
  13. Niedobitek, G., Young, L. S., Lau, R., Brooks, L., Greenspan, D., Greenspan, J. S. & Rickinson, A. B. ( 1991; ). Epstein–Barr virus infection in oral hairy leukoplakia: virus replication in the absence of a detectable latent phase. Journal of General Virology 72, 3035-3046.[CrossRef]
    [Google Scholar]
  14. Niedobitek, G., Agathanggelou, A., Herbst, H., Whitehead, L., Wright, D. H. & Young, L. S. ( 1997; ). Epstein–Barr virus (EBV) infection in infectious mononucleosis: virus latency, replication and phenotype of EBV-infected cells. Journal of Pathology 182, 151-159.[CrossRef]
    [Google Scholar]
  15. Niedobitek, G., Agathanggelou, A., Steven, N. & Young, L. S. ( 2000; ). Epstein–Barr virus (EBV) in infectious mononucleosis: detection of the virus in tonsillar B lymphocytes but not in desquamated oropharyngeal epithelial cells. Molecular Pathology 53, 37-42.[CrossRef]
    [Google Scholar]
  16. Sixbey, J. W., Nedrud, J. G., Raab-Traub, N., Hanes, R. A. & Pagano, J. S. ( 1984; ). Epstein–Barr virus replication in oropharyngeal epithelial cells. New England Journal of Medicine 310, 1225-1230.[CrossRef]
    [Google Scholar]
  17. Thomas, J. A., Felix, D. H., Wray, D., Southam, J. C., Cubie, H. A. & Crawford, D. H. ( 1991; ). Epstein–Barr virus gene expression and epithelial cell differentiation in oral hairy leukoplakia. American Journal of Pathology 139, 1369-1380.
    [Google Scholar]
  18. Thorley-Lawson, D. A. & Babcock, G. J. ( 1999; ). A model for persistent infection with Epstein–Barr virus: the stealth virus of human B cells. Life Sciences 14, 1433-1453.
    [Google Scholar]
  19. van Grunsven, W. M., van Heerde, E. C., de Haard, H. J., Spaan, W. J. & Middeldorp, J. M. ( 1993; ). Gene mapping and expression of two immunodominant Epstein–Barr virus capsid proteins. Journal of Virology 67, 3908-3916.
    [Google Scholar]
  20. Walling, D. M., Flaitz, C. M., Nichols, C. M., Hudnall, S. D. & Adler-Storthz, K. ( 2001; ). Persistent productive Epstein–Barr virus replication in normal epithelial cells in vivo. Journal of Infectious Diseases 184, 1499-1507.[CrossRef]
    [Google Scholar]
  21. Young, L. S., Lau, R., Rowe, M., Niedobitek, G., Packham, G., Shanaham, F., Rowe, D. T., Greenspan, D., Greenspan, J. S., Rickinson, A. B. & Farrell, P. J. ( 1991; ). Differentiation-associated expression of the Epstein–Barr virus BZLF1 transactivator protein in oral ‘hairy’ leukoplakia. Journal of Virology 65, 2868-2874.
    [Google Scholar]
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