1887

Abstract

The transcriptional switch region of temperate bacteriophage A2 contains three similar 20 bp operator subsites, O, O and O, which are interspersed between the divergent promoters and . The Cro protein binds initially to O, which overlaps the −35 region of , excluding the RNA polymerase (σ-RNAP) from it. This results in the switching off of transcription and directs the incoming phage into the lytic cycle. At higher concentrations, Cro also binds to O and/or O, which overlap , probably introducing a bend in the intervening DNA. This interaction induces DNA looping, which provokes the subsequent displacement of σ-RNAP from . Consequently, Cro abolishes the binding of σ-RNAP to the genetic switch of A2 and, presumably, its own synthesis, contributing indirectly to the entry of phage development into its late stages.

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2002-11-01
2019-10-18
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