1887

Journal of General Virology

Volume 83, Issue 11

Characterization of multicapsid nucleopolyhedrovirus ORF17/18, a homologue of granulovirus ORF129 Free

Abstract

multicapsid nucleopolyhedrovirus (SeMNPV) contains a number of genes with a homologue found so far only in a distantly related baculovirus. One of these, SeMNPV ORF17/18 (Se17/18) shares 55% amino acid similarity to ORF129 of granulovirus (XcGV). Se17/18 was transcribed in cultured 301 cells, as a polyadenylated transcript of 1·1 kb. 5′-RACE analysis demonstrated that Se17/18 transcripts started at 134, 131 and 126 nt upstream of the putative translational start codon. These sites overlap with a baculovirus consensus early promoter motif. Se17/18 transcripts were detected by Northern blot analysis and RT–PCR with increasing abundance from 8 h to 24 h post infection (p.i.) and still present until 72 h p.i. A C-terminal GFP-fusion protein of Se17/18 was primarily localized in the cytoplasm of Se301 and Sf21 cells. A chicken polyclonal antiserum was raised that reacted specifically to Se17/18 protein produced in . However, no immunoreactive protein was detected in SeMNPV-infected Se301 cells and larvae, neither in concentrated BV and ODV preparations. These observations and the inability to detect a C-terminal GFP-fusion protein of Se17/18 in Se301 cells using a GFP antibody suggest that Se17/18 protein is present, if at all, in spurious amounts. Based on the low homology of the Se17/18 protein to (methyl) transferases its possible involvement in transcription regulation is discussed.

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© Microbiology Society
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2002-11-01
2020-01-21
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Characterization of Spodoptera exigua multicapsid nucleopolyhedrovirus ORF17/18, a homologue of Xestia c-nigrum granulovirus ORF129
J. Gen. Virol. 83, 2857 (2002); https://doi.org/10.1099/0022-1317-83-11-2857
/content/journal/jgv/10.1099/0022-1317-83-11-2857
/content/journal/jgv/10.1099/0022-1317-83-11-2857
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