1887

Abstract

type 1 (PCV1) contains two major open reading frames encoding the replication initiator proteins, Rep and Rep′, and the structural protein, Cap. The promoters of these two genes (P and P) have been mapped. P is located within the open reading frame (nt 1328–1252). P has been mapped to the intergenic region immediately upstream of the gene (nt 640–796) and overlaps the origin of replication of PCV1. Although binding of both gene products to a fragment containing P and the overlapping origin of replication has been reported, only the full-length Rep protein repressed P, while the spliced isoform Rep′ did not. P repression is mediated by binding of the Rep protein to the two inner hexamers, H1 and H2, located in the origin of PCV1, whereas binding of Rep to hexamers H3 and H4 was not necessary. Use of Rep mutants indicated that the conserved rolling-circle replication domain II as well as the P loop are essential for repression of P. In contrast to P, transcription of P was not influenced by viral proteins. Additionally, the ratio of the and transcripts was analysed. Twelve hours after transfection of PK15 cells with an infectious clone of PCV1, similar amounts of both transcripts were detected, but later the amount of the two transcripts varied, indicating a balanced expression of the two transcripts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-11-2743
2002-11-01
2020-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/11/0832743a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-11-2743&mimeType=html&fmt=ahah

References

  1. Arguello-Astorga G. R., Ruiz-Medrano R.. 2001; An iteron-related domain is associated to Motif 1 in the replication proteins of geminiviruses: identification of potential interacting amino acid-base pairs by a comparative approach. Archives of Virology146:1465–1485
    [Google Scholar]
  2. Bassami M. R., Berryman D., Wilcox G. E., Raidal S. R.. 1998; Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus. Virology249:453–459
    [Google Scholar]
  3. Gibbs M. J., Weiller G. F.. 1999; Evidence that a plant virus switched hosts to infect a vertebrate and then recombined with a vertebrate-infecting virus. Proceedings of the National Academy of Sciences, USA96:8022–8027
    [Google Scholar]
  4. Hamel A. L., Lin L. L., Nayar G. P.. 1998; Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. Journal of Virology72:5262–5267
    [Google Scholar]
  5. Kato A., Fujino M., Nakamura T., Ishihama A., Otaki Y.. 1995; Gene organization of chicken anemia virus. Virology209:480–488
    [Google Scholar]
  6. MacGregor G. R., Mogg A. E., Burke J. F., Caskey C. T.. 1987; Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression of the bacterial gene. Somatic Cell and Molecular Genetics13:253–265
    [Google Scholar]
  7. McNulty M., Dale J., Lukert P., Mankertz A., Randles J., Todd D.. 2000; Circoviridae . In Seventh Report of the International Committee on Taxonomy of Viruses pp299–303 Edited by van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., Maniloff J., Mayo M. A., McGeoch D. J., Pringle C. R., Wickner R. B.. San Diego: Academic Press;
    [Google Scholar]
  8. Mahe D., Blanchard P., Truong C., Arnauld C., Le Cann P., Cariolet R., Madec F., Albina E., Jestin A.. 2000; Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. Journal of General Virology81:1815–1824
    [Google Scholar]
  9. Mankertz A., Hillenbrand B.. 2001; Replication of porcine circovirus type 1 requires two proteins encoded by the viral rep gene. Virology279:429–438
    [Google Scholar]
  10. Mankertz A., Persson F., Mankertz J., Blaess G., Buhk H. J.. 1997; Mapping and characterization of the origin of DNA replication of porcine circovirus. Journal of Virology71:2562–2566
    [Google Scholar]
  11. Mankertz A., Mankertz J., Wolf K., Buhk H. J.. 1998a; Identification of a protein essential for replication of porcine circovirus. Journal of General Virology79:381–384
    [Google Scholar]
  12. Mankertz J., Buhk H. J., Blaess G., Mankertz A.. 1998b; Transcription analysis of porcine circovirus (PCV). Virus Genes16:267–276
    [Google Scholar]
  13. Mankertz A., Hattermann K., Ehlers B., Soike D.. 2000; Cloning and sequencing of columbid circovirus (CoCV), a new circovirus from pigeons. Archives of Virology145:2469–2480
    [Google Scholar]
  14. Nawagitgul P., Morozov I., Bolin S. R., Harms P. A., Sorden S. D., Paul P. S.. 2000; Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. Journal of General Virology81:2281–2287
    [Google Scholar]
  15. Nishigawa H., Miyata S., Oshima K., Sawayanagi T., Komoto A., Kuboyama T., Matsuda I., Tsuchizaki T., Namba S.. 2001; In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteins. Microbiology147:507–513
    [Google Scholar]
  16. Phenix K. V., Weston J. H., Ypelaar I., Lavazza A., Smyth J. A., Todd D., Wilcox G. E., Raidal S. R.. 2001; Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae . Journal of General Virology82:2805–2809
    [Google Scholar]
  17. Sanada Y., Sanada N., Kubo M.. 1999; Electron microscopical observations of psittacine beak and feather disease in an Umbrella cockatoo ( Cacatua alba ). Journal of Veterinary Medical Science61:1063–1065
    [Google Scholar]
  18. Sawadogo M., Roeder R. G.. 1985; Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region. Cell43:165–175
    [Google Scholar]
  19. Soike D., Hattermann K., Albrecht K., Segales J., Domingo M., Schmitt C., Mankertz A.. 2001; A diagnostic study on Columbid Circovirus (CoCV) infection. Avian Pathology30:605–611
    [Google Scholar]
  20. Steinfeldt T., Finsterbusch T., Mankertz A.. 2001; Rep and Rep′ protein of porcine circovirus type 1 bind to the origin of replication in vitro. Virology291:152–160
    [Google Scholar]
  21. Tischer I., Mields W., Wolff D., Vagt M., Griem W.. 1986; Studies on epidemiology and pathogenicity of porcine circovirus. Archives of Virology91:271–276
    [Google Scholar]
  22. Todd D., Weston J. H., Soike D., Smyth J. A.. 2001; Genome sequence determinations and analyses of novel circoviruses from goose and pigeon. Virology286:354–362
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-11-2743
Loading
/content/journal/jgv/10.1099/0022-1317-83-11-2743
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error