1887

Abstract

Early after infection, lactate dehydrogenase-elevating virus (LDV) alters the immune system by polyclonally activating B lymphocytes, which leads to IgG2a-restricted hypergammaglobulinaemia, and by suppressing the secretion of Th2 cytokines. Considering that these alterations may involve cells of the innate immune system and cytokines such as interferon-gamma (IFN-γ), we analysed the effect of LDV on natural killer (NK) cells. Within a few days of infection, a strong and transient NK cell activation, characterized by enhanced γ message expression and cytolysis, was observed. LDV triggered a large increase in serum IFN-γ levels. Because NK cells and IFN-γ may participate in the defence against virus infection, we analysed their possible role in the control of LDV titres with a new agglutination assay. Our results indicate that neither the activation of NK cells nor the IFN-γ secretion affect the early and rapid virus replication that follows LDV inoculation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-11-2709
2002-11-01
2020-10-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/11/0832709a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-11-2709&mimeType=html&fmt=ahah

References

  1. Arase H., Saito T., Phillips J. H., Lanier L. L.. 2001; The mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (α2 integrin, very late antigen-2). Journal of Immunology167:1141–1144
    [Google Scholar]
  2. Bancroft G. J., Shellam G. R., Chalmer J. E.. 1981; Genetic influences on the augmentation of natural killer (NK) cells during murine cytomegalovirus infection: correlation with patterns of resistance. Journal of Immunology126:988–994
    [Google Scholar]
  3. Biron C. A.. 1997; Activation and function of natural killer cell responses during viral infections. Current Opinion in Immunology9:24–34
    [Google Scholar]
  4. Biron C. A., Nguyen K. B., Pien G. C., Cousens L. P., Salazar-Mather T. P.. 1999; Natural killer cells in antiviral defense: function and regulation by innate cytokines. Annual Review of Immunology17:189–220
    [Google Scholar]
  5. Cafruny W. A., Haven T. R., Lawson S. R., Wong G. H. W., Rowland R. R. R.. 1997; Inhibition of virus-induced age-dependent poliomyelitis by interferon-γ. Antiviral Research36:1–9
    [Google Scholar]
  6. Cafruny W. A., Bradley S. E., Rowland R. R. R.. 1999; Regulation of immune complexes during infection of mice with lactate dehydrogenase-elevating virus: studies with interferon-gamma gene knockout and tolerant mice. Viral Immunology12:163–173
    [Google Scholar]
  7. Coulie P. G., Coutelier J.-P., Uyttenhove C., Lambotte P., Van Snick J.. 1985; In vivo suppression of T-dependent antibody responses by treatment with a monoclonal anti-L3T4 antibody. European Journal of Immunology15:638–640
    [Google Scholar]
  8. Coutelier J.-P.. 1991; Enhancement of IgG production elicited in mice by treatment with anti-CD8 antibody. European Journal of Immunology21:2617–2620
    [Google Scholar]
  9. Coutelier J.-P., Van Snick J.. 1985; Isotypically restricted activation of B lymphocytes by lactic dehydrogenase virus. European Journal of Immunology15:250–255
    [Google Scholar]
  10. Coutelier J.-P., Van Roost E., Lambotte P., Van Snick J.. 1986; The murine antibody response to lactate dehydrogenase-elevating virus. Journal of General Virology67:1099–1108
    [Google Scholar]
  11. Coutelier J.-P., Van Broeck J., Wolf S. F.. 1995; Interleukin-12 gene expression after viral infection in the mouse. Journal of Virology69:1955–1958
    [Google Scholar]
  12. Dialynas D. P., Wilde D. B., Marrack P., Pierres A., Wall K. A., Havran W., Otten G., Loken M. R., Pierres M., Kappler J., Fitch F. W.. 1983; Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II antigen-reactivity. Immunological Reviews74:29–56
    [Google Scholar]
  13. Doherty T. M., Seder R. A., Sher A.. 1996; Induction and regulation of IL-15 expression in murine macrophages. Journal of Immunology156:735–741
    [Google Scholar]
  14. El Azami El Idrissi M., Mazza G., Monteyne P., Elson C. J., Day M. J., Pfau C. J., Coutelier J.-P.. 1998; Lymphocytic choriomeningitis virus-induced alterations of T helper-mediated responses in mice developing autoimmune hemolytic anemia during the course of infection. Proceedings of the Society for Experimental Biology and Medicine218:349–356
    [Google Scholar]
  15. Even C., Rowland R. R. R., Plagemann P. G. W.. 1995; Cytotoxic T cells are elicited during acute infection of mice with lactate dehydrogenase-elevating virus but disappear during the chronic phase of infection. Journal of Virology69:5666–5676
    [Google Scholar]
  16. Galanti L. M., Dell’Omo J., Wanet B., Guarin J.-L., Jamart J., Garrino M.-G., Masson P. L., Cambiaso C. L.. 1997; Particle counting assay for anti-toxoplasma IgG antibodies. Comparison with four automated commercial enzyme-linked immunoassays. Journal of Immunological Methods207:195–201
    [Google Scholar]
  17. Heremans H., Billiau A., Coutelier J.-P., De Somer P.. 1987; The inhibition of endotoxin-induced local inflammation by LDH virus or LDH virus-infected tumors is mediated by interferon. Proceedings of the Society for Experimental Biology and Medicine185:6–15
    [Google Scholar]
  18. Huang S., Hendriks W., Althage A., Hemmi S., Bluethmann H., Kamijo R., Vilcek J., Zinkernagel R. M., Aguet M.. 1993; Immune response in mice that lack the interferon-γ receptor. Science259:1742–1745
    [Google Scholar]
  19. Kasai M., Iwamori M., Nagai Y., Okumura K., Tada T.. 1980; A glycolipid on the surface of mouse natural killer cells. European Journal of Immunology10:175–180
    [Google Scholar]
  20. Koi M., Saito M., Ebina T., Ishida N.. 1981; Lactate dehydrogenase-elevating agent is responsible for interferon induction and enhancement of natural killer cell activity by inoculation of Ehrlich ascites carcinoma cells into mice. Microbiology and Immunology25:565–574
    [Google Scholar]
  21. Lauwerys B. R., Renauld J.-C., Houssiau F. A.. 1999; Synergistic proliferation and activation of natural killer cells by interleukin 12 and interleukin 18. Cytokine11:822–830
    [Google Scholar]
  22. Leclercq G., Willems J., Heremans H., Coutelier J.-P., Billiau A., Joniau M.. 1987; Cellular immunity changes caused by LDH virus: analogy with observations on neuroblastoma-bearing mice. International Journal of Cancer40:669–675
    [Google Scholar]
  23. Ledbetter J. A., Herzenberg L. A.. 1979; Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunological Reviews47:63–90
    [Google Scholar]
  24. Markine-Goriaynoff D., van der Logt J. T. M., Truyens C., Nguyen T. D., Heessen F. W. A., Bigaignon G., Carlier Y., Coutelier J.-P.. 2000; IFN-γ-independent IgG2a production in mice infected with viruses and parasites. International Immunology12:223–230
    [Google Scholar]
  25. Markine-Goriaynoff D., Nguyen T. D., Bigaignon G., Van Snick J., Coutelier J.-P.. 2001; Distinct requirements for IL-6 in polyclonal and specific Ig production induced by microorganisms. International Immunology13:1185–1192
    [Google Scholar]
  26. Masson P. L., Cambiaso C. L., Collet-Cassart D., Magnusson C. G. M., Richards C. B., Sindic C. J. M.. 1981; Particle counting immunoassay (PACIA). Methods in Enzymology74:106–139
    [Google Scholar]
  27. Meite M., Léonard S., El Azami El Idrissi M., Izui S., Masson P. L., Coutelier J.-P.. 2000; Exacerbation of autoantibody-mediated hemolytic anemia by viral infection. Journal of Virology74:6045–6049
    [Google Scholar]
  28. Monteyne P., Van Broeck J., Van Snick J., Coutelier J.-P.. 1993; Inhibition by lactate dehydrogenase-elevating virus of in vivo interleukin 4 production during immunization with keyhole limpet haemocyanin. Cytokine5:394–397
    [Google Scholar]
  29. Monteyne P., Renauld J.-C., Van Broeck J., Dunne D. W., Brombacher F., Coutelier J.-P.. 1997; IL-4-independent regulation of in vivo IL-9 expression. Journal of Immunology159:2616–2623
    [Google Scholar]
  30. Natuk R. J., Welsh R. M.. 1987; Accumulation and chemotaxis of natural killer/large granular lymphocytes at sites of virus replication. Journal of Immunology138:877–883
    [Google Scholar]
  31. Notkins A. L., Mahar S., Scheele C., Goffman J.. 1966; Infectious virus-antibody complex in the blood of chronically infected mice. Journal of Experimental Medicine124:81–97
    [Google Scholar]
  32. Onyekaba C. O., Harty J. T., Even C., Hu B.-g., Plagemann P. G. W.. 1989; Persistent infection of mice by lactate dehydrogenase-elevating virus: effects of immunosuppression on virus replication and antiviral immune responses. Virus Research14:297–316
    [Google Scholar]
  33. Pien G. C., Biron C. A.. 2000; Compartmental differences in NK cell responsiveness to IL-12 during lymphocytic choriomeningitis virus infection. Journal of Immunology164:994–1001
    [Google Scholar]
  34. Plagemann P. G. W., Rowland R. R. R., Even C., Faaberg K. S.. 1995; Lactate dehydrogenase-elevating virus: an ideal persistent virus?. Springer Seminars in Immunopathology17:167–186
    [Google Scholar]
  35. Rowson K. E. K., Mahy B. W. J.. 1975; Lactic dehydrogenase virus. Virology Monographs , vol. 13 New York: Springer-Verlag;
    [Google Scholar]
  36. Smith A. L., Barthold S. W., de Souza M. S., Bottomly K.. 1991; The role of gamma interferon in infection of susceptible mice with murine coronavirus, MHV-JHM. Archives of Virology121:89–100
    [Google Scholar]
  37. Stueckemann J. A., Ritzi D. M., Holth M., Smith M. S., Swart W. J., Cafruny W. A., Plagemann P. G. W.. 1982; Replication of lactate dehydrogenase-elevating virus in macrophages. 1. Evidence for cytocidal replication. Journal of General Virology59:245–262
    [Google Scholar]
  38. Trinchieri G.. 1989; Biology of natural killer cells. Advances in Immunology47:187–376
    [Google Scholar]
  39. van den Broek M. F., Spörri R., Even C., Plagemann P. G. W., Hänseler E., Hengartner H., Zinkernagel R. M.. 1997; Lactate dehydrogenase-elevating virus (LDV). Lifelong coexistence of virus and LDV-specific immunity. Journal of Immunology159:1585–1588
    [Google Scholar]
  40. Welsh R. M., Zinkernagel R. M.. 1977; Heterospecific cytotoxic cell activity induced during the first three days of acute lymphocytic choriomeningitis virus infection in mice. Nature268:646–648
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-11-2709
Loading
/content/journal/jgv/10.1099/0022-1317-83-11-2709
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error