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Journal of General Virology header logo Journal of General Virology

Volume 83, Issue 11

Characterization of the receptor-destroying enzyme activity from infectious salmon anaemia virus No Access

Abstract

Infectious salmon anaemia virus (ISAV) infects cells via the endocytic pathway and, like many other enveloped viruses, ISAV contains a receptor-destroying enzyme. We have analysed this acetylesterase activity with respect to substrate specificity, enzyme kinetics, inhibitors, temperature and pH stability. The ISAV acetylesterase was inhibited by di-isopropyl fluorophosphate (DFP) in a dose-dependent fashion but not by other known hydrolase inhibitors, suggesting that a serine residue is part of the active site. The pH optimum of the enzyme was in the range 7·5–8·0 and the enzymatic activity was lessened at temperatures above 40 °C. The effect of DFP on agglutination/elution of erythrocytes by ISAV demonstrated that the acetylesterase activity is the bona fide receptor-destroying enzyme. A haemadsorption assay was used to analyse whether the esterase was active on the surface of infected cells or not.

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© Microbiology Society
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2002-11-01
2025-03-20
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/content/journal/jgv/10.1099/0022-1317-83-11-2693
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Characterization of the receptor-destroying enzyme activity from infectious salmon anaemia virus
J. Gen. Virol. 83, 2693 (2002); https://doi.org/10.1099/0022-1317-83-11-2693
/content/journal/jgv/10.1099/0022-1317-83-11-2693
/content/journal/jgv/10.1099/0022-1317-83-11-2693
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