1887

Abstract

Transmission electron micrographs of cells induced by cloned lysis genes from RNA bacteriophages GA (group A-II) and SP (group B-IV) revealed various morphological aspects of intermediates of lysing cells. Cells induced by the SP lysis gene became stretched and also tapered in shape and fragmentation of parts of the cells had also occurred. Cells induced by the GA lysis gene showed many ballooning structures on the cell surfaces and others leaked material through the cell wall. Some balloon-like structures also appeared on the surfaces of cells induced by the cloned lysis gene of RNA phage SP and material also appeared to be leaking through the cell wall in the photographs. The lysing cells observed by transmission electron microscopy showed various morphological aspects of intermediates of the lysing process.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-10-2601
2002-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/10/0832601a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-10-2601&mimeType=html&fmt=ahah

References

  1. Adhin M. R., van Duin J. 1989; Translational regulation of the lysis gene in RNA bacteriophage fr requires a UUG initiation codon. Molecular & General Genetics 218:137–142
    [Google Scholar]
  2. Adhin M. R., Hirashima A., van Duin J. 1989; Nucleotide sequence from the ssRNA bacteriophage JP34 resolves the discrepancy between serological and biophysical classification. Virology 170:238–242
    [Google Scholar]
  3. Bernard H.-U., Remaut E., Hershfield M. V., Das H. K., Helinski D. R., Yanofsky C., Franklin N. 1979; Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda pL promoter. Gene 5:59–76
    [Google Scholar]
  4. Bernhardt T. G., Roof W. D., Young R. 2000; Genetic evidence that the bacteriophage ϕX174 lysis protein inhibits cell wall synthesis. Proceedings of the National Academy of Sciences, USA 97:4297–4302
    [Google Scholar]
  5. Bernhardt T. G., Struck D. K., Young R. 2001a; The lysis protein E of ϕX174 is a specific inhibitor of the MraY-catalyzed step in peptidoglycan synthesis. Journal of Biological Chemistry 276:6093–6097
    [Google Scholar]
  6. Bernhardt T. G., Wang I.-N., Struck D. K., Young R. 2001b; A protein antibiotic in the phage Qβ virion: diversity in lysis targets. Science 292:2326–2329
    [Google Scholar]
  7. Coleman J., Inouye M., Atkins J. 1983; Bacteriophage MS2 lysis protein does not require coat protein to mediate cell lysis. Journal of Bacteriology 153:1098–1100
    [Google Scholar]
  8. De Petris S. 1967; Ultrastructure of the cell wall of Escherichia coli and chemical nature of its constituent layers. Journal of Ultrastructure Research 19:45–83
    [Google Scholar]
  9. Furuse K., Hirashima A., Harigai H., Ando A., Watanabe K., Kurosawa K., Inokuchi Y., Watanabe I. 1979; Grouping of RNA coliphages based on analysis of the sizes of their RNAs and proteins. Virology 97:328–341
    [Google Scholar]
  10. Groeneveld H., Oudot F., van Duin J. 1996; RNA phage KU1 has an insertion of 18 nucleotides in the start codon of its lysis gene. Virology 218:141–147
    [Google Scholar]
  11. Inokuchi Y., Takahashi R., Hirose T., Inayama S., Jacobson A. B., Hirashima A. 1986; The complete nucleotide sequence of the group II RNA coliphage GA. Journal of Biochemistry 99:1169–1180
    [Google Scholar]
  12. Inokuchi Y., Jacobson A. B., Hirose T., Inayama S., Hirashima A. 1988; Analysis of the complete nucleotide sequence of the group IV RNA coliphage SP. Nucleic Acids Research 16:6205–6221
    [Google Scholar]
  13. Karnik S., Billeter M. 1983; The lysis function of RNA bacteriophage Qβ is mediated by the maturation (A2) protein. EMBO Journal 2:1521–1526
    [Google Scholar]
  14. Kastelein R. A., Remaut E., Fiers W., van Duin J. 1982; Lysis gene expression of RNA phage MS2 depends on a frameshift during translation. Nature 276:35–41
    [Google Scholar]
  15. Miyake T., Shiba T., Sakurai T., Watanabe I. 1969; Isolation and properties of two new RNA phages SP and FI. Japanese Journal of Microbiology 13:375–382
    [Google Scholar]
  16. Priano C., Arora R., Butke J., Mills D. R. 1995; A complete plasmid-based complementation system for RNA coliphage Qβ: three proteins of bacteriophages Qβ (group III) and SP (group IV) can be interchanged. Journal of Molecular Biology 249:283–297
    [Google Scholar]
  17. Remaut E., Stanssens P., Fiers W. 1983; Inducible high level synthesis of mature human fibroblast interferon in Escherichia coli. Nucleic Acids Research 11:4677–4688
    [Google Scholar]
  18. Rietsch A., Bläsi U. 1993; Non-specific hole formation in the Escherichia coli inner membrane by λS proteins is independent of cellular secY and secA functions and of the proportion of membrane acidic phospholipids. FEMS Microbiology Letters 107:101–105
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  21. Spurr A. R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26:31–43
    [Google Scholar]
  22. van Duin J. 1988; Single-stranded RNA bacteriophages. In The Bacteriophages pp 117–167 Edited by Calendar R. New York: Plenum;
    [Google Scholar]
  23. Watanabe I., Miyake T., Sakurai T., Shiba T., Ohno T. 1967a; Isolation and grouping of RNA phages. Proceedings of the Japan Academy 43:204–209
    [Google Scholar]
  24. Watanabe I., Nishihara T., Kaneko H., Sakurai T., Osawa S. 1967b; Group characteristics of RNA phages. Proceedings of the Japan Academy 43:210–213
    [Google Scholar]
  25. Winter R. B., Gold L. 1983; Overproduction of bacteriophage Qβ maturation (A2) protein leads to cell lysis. Cell 33:877–885
    [Google Scholar]
  26. Witte A., Reisinger G. R., Saeckl W., Wanner G., Lubitz W. 1989; Characterization of Escherichia coli lysis using a family of chimeric E–L genes. FEMS Microbiology Letters 164:159–167
    [Google Scholar]
  27. Young R. 1992; Bacteriophage lysis: mechanism and regulation. Microbiological Reviews 56:430–481
    [Google Scholar]
  28. Young R., Wang I.-N., Roof W. D. 2000; Phages will out: strategies of host cell lysis. Trends in Microbiology 8:120–128
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-10-2601
Loading
/content/journal/jgv/10.1099/0022-1317-83-10-2601
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error