RT Journal Article SR Electronic(1) A1 Blanch, Ewan W. A1 Hecht, Lutz A1 Syme, Christopher D. A1 Volpetti, Vito A1 Lomonossoff, George P. A1 Nielsen, Kurt A1 Barron, Laurence D.YR 2002 T1 Molecular structures of viruses from Raman optical activity JF Journal of General Virology, VO 83 IS 10 SP 2593 OP 2600 DO https://doi.org/10.1099/0022-1317-83-10-2593 PB Microbiology Society, SN 1465-2099, AB A vibrational Raman optical activity (ROA) study of a range of different structural types of virus exemplified by filamentous bacteriophage fd, tobacco mosaic virus, satellite tobacco mosaic virus, bacteriophage MS2 and cowpea mosaic virus has revealed that, on account of its sensitivity to chirality, ROA is an incisive probe of their aqueous solution structures at the molecular level. Protein ROA bands are especially prominent from which, as we have shown by comparison with the ROA spectra of proteins with known structures and by using a pattern recognition program, the folds of the major coat protein subunits may be deduced. Information about amino acid side-chain conformations, exemplified here by the determination of the sign and magnitude of the torsion angle χ2,1 for tryptophan in fd, may also sometimes be obtained. By subtracting the ROA spectrum of the empty protein capsid (top component) of cowpea mosaic virus from those of the intact middle and bottom-upper components separated by means of a caesium chloride density gradient, the ROA spectrum of the viral RNA was obtained, which revealed that the RNA takes up an A-type single-stranded helical conformation and that the RNA conformations in the middle and bottom-upper components are very similar. This information is not available from the X-ray crystal structure of cowpea mosaic virus since no nucleic acid is visible., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-83-10-2593