1887

Abstract

The potyvirus (PRSV) is found throughout the tropics and subtropics. Its P biotype is a devastating pathogen of papaya crops and its W biotype of cucurbits. PRSV-P is thought to arise by mutation from PRSV-W. However, the relative impact of mutation and movement on the structure of PRSV populations is not well characterized. To investigate this, we have determined the coat protein sequences of isolates of both biotypes of PRSV from Vietnam (50), Thailand (13), India (1) and the Philippines (1), and analysed them together with 28 PRSV sequences already published, so that we can better understand the molecular epidemiology and evolution of PRSV. In Thailand, variation was greater among PRSV-W isolates (mean nucleotide divergence 7·6%) than PRSV-P isolates (mean 2·6%), but in Vietnamese populations the P and W biotypes were more but similarly diverse. Phylogenetic analyses of PRSV also involving its closest known relative, , indicate that PRSV may have originated in Asia, particularly in the Indian subcontinent, as PRSV populations there are most diverse and hence have probably been present longest. Our analyses show that mutation, together with local and long-distance movement, contributes to population variation, and also confirms an earlier conclusion that populations of the PRSV-P biotype have evolved on several occasions from PRSV-W populations.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-10-2575
2002-10-01
2024-11-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/10/0832575a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-10-2575&mimeType=html&fmt=ahah

References

  1. Bateson M. F., Henderson J., Chaleeprom W., Gibbs A. J., Dale J. L. 1994; Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P in Australia. Journal of General Virology 75:3547–3553
    [Google Scholar]
  2. Bousalem M., Douzery E. J. P., Fargette D. 2000; High genetic diversity, distant phylogenetic relationships and intraspecies recombination events among natural populations of Yam mosaic virus : a contribution to understanding potyvirus evolution. Journal of General Virology 81:243–255
    [Google Scholar]
  3. Cervera M. T., Riechmann J. L., Martin M. T., Garcia J. A. 1993; 3′-Terminal sequence of the plum pox virus PS and ǒ6 isolates: evidence for RNA recombination within the potyvirus group. Journal of General Virology 74:329–334
    [Google Scholar]
  4. Chaleeprom W. 1997; Genome analysis of Papaya ringspot potyvirus and a related virus. PhD thesis Queensland University of Technology; Australia:
  5. Davis M. J., Ying Z. 1999; Genetic diversity of the Papaya ringspot virus in Florida. Proceedings of the Florida State Horticultural Society 112:194–196
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  7. Feng D. F., Doolittle R. F. 1987; Progressive sequence alignment as a prerequisite to correct phylogenetic trees. Journal of Molecular Evolution 25:351–360
    [Google Scholar]
  8. Garcia-Arenal F., Fraile A., Malpica J. M. 2001; Variability and genetic structure of plant virus populations. Annual Review of Phytopathology 39:157–186
    [Google Scholar]
  9. Gibbs M. J., Armstrong J. S., Gibbs A. J. 2000; Sister scanning: a Monte Carlo procedure for assessing signals in recombinant sequences. Bioinformatics 16:573–582
    [Google Scholar]
  10. Gonsalves D. 1998; Control of papaya ringspot virus in papaya: a case study. Annual Review of Phytopathology 36:415–437
    [Google Scholar]
  11. Greber R. S. 1978; Watermelon mosaic virus 1 and 2 in Queensland cucurbit crops. Australian Journal of Agricultural Research 29:1235–1245
    [Google Scholar]
  12. IBPGR 1983 Genetic Resources of Cucurbitaceae Rome, Italy: Crop Genetic Resources Centre, Plant Production and Protection Division, Food and Agriculture Organization of the United Nations;
    [Google Scholar]
  13. Jain R. K., Pappu H. R., Pappu S. S., Varma A., Ram R. D. 1998; Molecular characterisation of Papaya ringspot potyvirus isolates from India. Annals of Applied Biology 132:413–425
    [Google Scholar]
  14. Jensen D. D. 1949; Papaya virus diseases with special reference to papaya ringspot. Phytopathology 39:191–211
    [Google Scholar]
  15. Lecoq H., Dafalla G., Desbiez C., Wipf-Scheibel C., Delécolle B., Lanina T., Ullah Z., Grumet R. 2000; Biological and molecular characterization of Moroccan watermelon mosaic virus and a potyvirus isolate from Eastern Sudan. Plant Disease 85:547–552
    [Google Scholar]
  16. Page R. D. M. 1996; treeview: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12:357–358
    [Google Scholar]
  17. Persley D. M. 1998; Identification, epidemiology and control of papaya ringspot virus, recently recorded in papaya (Carica papaya) in Australia. Masters thesis Queensland University of Technology; Australia:
  18. Purcifull D. E., Edwardson J. R., Hiebert E., Gonsalves D. 1984; Papaya ringspot virus. CMI/AAB Descriptions of Plant Viruses, no. 292 Wallingford, UK: CAB International;
    [Google Scholar]
  19. Purseglove J. W. 1968 Caricaceae . Tropical Crops: Dicotyledons London: Longman;
    [Google Scholar]
  20. Quemada H., Hostis B. L., Gonsalves D., Reardon I. M., Heinrikson R., Hiebert E. L., Sieu L. C., Slightom J. L. 1990; The nucleotide sequence of the 3′ terminal regions of papaya ringspot virus strains W and P. Journal of General Virology 71:203–210
    [Google Scholar]
  21. Quiot-Douine L., Lecoq H., Quiot J. B., Pitrat M., Labonne G. 1990; Serological and biological variability of virus isolates related to strains of papaya ringspot virus. Phytopathology 80:256–263
    [Google Scholar]
  22. Rezende J. A. M., Pacheco D. A. 1998; Control of papaya ringspot virus type-W in zucchini squash by cross protection in Brazil. Plant Disease 82:171–175
    [Google Scholar]
  23. Shukla D. D., Ward W. W., Brunt A. A. 1994 The Potyviridae Wallingford, UK: CAB International;
    [Google Scholar]
  24. Silva-Rosales L., Becerra-Leor N., Ruiz-Castro S., Téliz-Ortiz D., Noa-Carrazana J. C. 2000; Coat protein sequence comparisons of three Mexican isolates of Papaya ringspot virus with other geographical isolates reveal a close relationship to American and Australian isolates. Archives of Virology 145:835–843
    [Google Scholar]
  25. Srisomchai T. 1975 Studies on papaya ringspot virus disease. Office of Northeast Agriculture and Co-operative Annual Report, Tha Phra, Khon Kaen, Thailand (In Thai.)
    [Google Scholar]
  26. Strimmer K., von Haeseler A. 1996; Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Molecular Biology and Evolution 13:964–969
    [Google Scholar]
  27. Strimmer K., von Haeseler A. 1997; Likelihood-mapping: a simple method to visualize phylogenetic content of a sequence alignment. Proceedings of the National Academy of Sciences, USA 94:6815–6819
    [Google Scholar]
  28. Tennant P. F., Gonsalves C., Ling K. S., Fitch M., Manshardt R., Slightom J. L., Gonslaves D. 1994; Differential protection against papaya ringspot virus isolates in coat protein gene transgenic papaya and classically cross-protected papaya. Phytopathology 84:1359–1366
    [Google Scholar]
  29. Thomas J. E., Dodman R. L. 1993; The first record of papaya ringspot virus-type P in Australia. Australian Plant Pathology 22:2–7
    [Google Scholar]
  30. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24:4876–4882
    [Google Scholar]
  31. Tomlinson J. A. 1987; Epidemiology and control of virus diseases and vegetables. Annals of Applied Biology 110:661–681
    [Google Scholar]
  32. Wang C. H., Bau H. J., Yeh S. D. 1994; Comparison of the nuclear inclusion B protein and coat protein genes of five papaya ringspot virus strains distinct in geographic origin and pathogenicity. Phytopathology 84:1205–1210
    [Google Scholar]
  33. Ward C. W., Weiller G. F., Shukla D. D., Gibbs A. J. 1995; Molecular systematics of the Potyviridae , the largest plant virus family. In Molecular Basis of Virus Evolution pp 477–497 Edited by Gibbs A., Calisher C. H., Garcia-Arenal F. Cambridge: Cambridge University Press;
    [Google Scholar]
  34. Yeh S. D., Gonsalves D. 1994; Practice and perspectives of control of papaya ringspot virus by cross protection. Advances in Disease Vector Research 10:237–257
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-83-10-2575
Loading
/content/journal/jgv/10.1099/0022-1317-83-10-2575
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error