1887

Abstract

We investigated the presence of the measles virus genome in order to identify asymptomatic infections in the adult population. Bone-marrow aspirates were obtained from 179 patients, 20–96 years of age, for the diagnosis of malignant diseases (29 with malignant lymphoma, 28 with acute leukaemia, 21 with myelodysplastic syndrome, five with multiple myeloma and 96 with other diseases). The measles virus genome was detected in 17 (9·5%) of 179 individuals by RT–PCR and 28 (15·6%) through hybridization. The genomes detected in bone marrow were all in the same cluster, D5, the strain circulating during the study period, and no evidence of persistent infection was obtained. We conclude that asymptomatic infections of measles virus are common in adults and the presence of the measles virus genome would not be related to the pathogenesis of illness.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-10-2485
2002-10-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/10/0832485a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-10-2485&mimeType=html&fmt=ahah

References

  1. Afzal, M. A., Minor, P. D., Begley, J., Bentley, M. L., Armitage, E., Ghosh, S. & Ferguson, A. ( 1998; ). Absence of measles-virus genome in inflammatory bowel disease. Lancet 351, 646-647.[CrossRef]
    [Google Scholar]
  2. Chomczynski, P. & Sacchi, N. ( 1987; ). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162, 156-159.
    [Google Scholar]
  3. Ekbom, A., Wakefield, A. J., Zack, M. & Adami, H.-O. ( 1994; ). Perinatal measles infection and subsequent Crohn’s disease. Lancet 344, 508-510.[CrossRef]
    [Google Scholar]
  4. Farrington, C. P., Miller, E. & Taylor, B. ( 2001; ). MMR and autism: further evidence against a causal association. Vaccine 19, 3632-3635.[CrossRef]
    [Google Scholar]
  5. Helfand, R. F., Kim, D. K., Gary, H. E.Jr, Edwards, G. L., Bisson, G. P., Papania, M. J., Heath, J. L., Schaff, D. L., Bellini, W. J., Redd, S. C. & Anderson, L. J. ( 1998; ). Nonclassic measles infections in an immune population exposed to measles during a college bus trip. Journal of Medical Virology 56, 337-341.[CrossRef]
    [Google Scholar]
  6. Huiss, S., Damien, B., Schneider, F. & Muller, C. P. ( 1997; ). Characteristics of asymptomatic secondary immune responses to measles virus in late convalescent donors. Clinical and Experimental Immunology 109, 416-420.[CrossRef]
    [Google Scholar]
  7. Katayama, Y., Hotta, H., Nishimura, A., Tatsuno, Y. & Homma, M. ( 1995; ). Detection of measles virus nucleoprotein mRNA in autopsied brain tissues. Journal of General Virology 76, 3201-3204.[CrossRef]
    [Google Scholar]
  8. Kawashima, H., Mori, T., Takekuma, K., Hoshika, A., Hata, M. & Nakayama, T. ( 1996; ). Polymerase chain reaction detection of the hemagglutinin gene from an attenuated measles vaccine strain in the peripheral mononuclear cells of children with autoimmune hepatitis. Archives of Virology 141, 877-884.[CrossRef]
    [Google Scholar]
  9. Kawashima, H., Mori, T., Kashiwagi, Y., Takekuma, K., Hoshika, A. & Wakefield, A. ( 2000; ). Detection and sequencing of measles virus from peripheral mononuclear cells from patients with inflammatory bowel disease and autism. Digestive Diseases and Sciences 45, 723-729.[CrossRef]
    [Google Scholar]
  10. Markowitz, L. E., Preblud, S. R., Orenstein, W. A., Rovira, E. Z., Adams, N. C., Hawkins, C. E. & Hinman, A. R. ( 1989; ). Patterns of transmission in measles outbreaks in the United States, 1985–1986. New England Journal of Medicine 320, 75-81.[CrossRef]
    [Google Scholar]
  11. Muller, C. P., Huiss, S. & Schneider, F. ( 1996; ). Secondary immune responses in parents of children with recent measles. Lancet 348, 1379-1380.
    [Google Scholar]
  12. Nakayama, T., Mori, T., Yamaguchi, S., Sonoda, S., Asamura, S., Yamashita, R., Takeuchi, Y. & Urano, T. ( 1995; ). Detection of measles virus genome directly from clinical samples by reverse transcriptase-polymerase chain reaction and genetic variability. Virus Research 35, 1-16.[CrossRef]
    [Google Scholar]
  13. Reyes, M. A., de Borrero, M. F., Roa, J., Bergonzoli, G. & Saravia, N. G. ( 1987; ). Measles vaccine failure after documented seroconversion. Pediatric Infectious Disease Journal 6, 848-851.[CrossRef]
    [Google Scholar]
  14. Sonoda, S. & Nakayama, T. ( 2001; ). Detection of measles virus genome in lymphocytes from asymptomatic healthy children. Journal of Medical Virology 65, 381-387.[CrossRef]
    [Google Scholar]
  15. Takahashi, M., Nakayama, T., Kashiwagi, Y., Takami, T., Sonoda, S., Yamanaka, T., Ochiai, H., Ihara, T. & Tajima, T. ( 2000; ). A single genotype of measles virus is dominant whereas several genotypes of mumps virus are co-circulating. Journal of Medical Virology 62, 278-285.[CrossRef]
    [Google Scholar]
  16. Vardas, E. & Kreis, S. ( 1999; ). Isolation of measles virus from a naturally-immune, asymptomatically re-infected individual. Journal of Clinical Virology 13, 173-179.[CrossRef]
    [Google Scholar]
  17. Wakefield, A. J., Sim, R., Akbar, A. N., Pounder, R. E. & Dhillon, A. P. ( 1997; ). In situ immune responses in Crohn’s disease: a comparison with acute and persistent measles virus infection. Journal of Medical Virology 51, 90-100.[CrossRef]
    [Google Scholar]
  18. WHO ( 2001; ). Nomenclature for describing the genetic characteristics of wild-type measles viruses (update). Weekly Epidemiological Record 76, 242–247, 249–251.
    [Google Scholar]
  19. Yamaguchi, S. ( 1997; ). Identification of three lineages of wild measles virus by nucleotide sequence analysis of N, P, M, F, and L genes in Japan. Journal of Medical Virology 52, 113-120.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-10-2485
Loading
/content/journal/jgv/10.1099/0022-1317-83-10-2485
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error