1887

Abstract

A cytopathogenic coronavirus, serologically identified as porcine haemagglutinating encephalomyelitis virus (HEV), has recently been associated with acute outbreaks of wasting and encephalitis in nursing piglets from pig farms in southern Québec and Ontario, Canada. The 3′-terminal end of the genome of the prototype HEV-67N strain and that of the recent Québec IAF-404 field isolate, both propagated in HRT-18 cells, were sequenced. Overall, sequencing data indicated that HEV has remained antigenically and genetically stable since its first isolation in North America in 1962. Compared with the prototype strain of bovine enteropathogenic coronavirus (BCoV), HEV, as well as the human respiratory coronavirus (HCoV-OC43) showed a major deletion in their ORF4 gene. Deduced amino acid sequences for both HEV strains revealed 89/88, 80, 93/92 and 95/94% identities with the structural proteins HE, S, M and N of BCoV and HCoV-OC43, respectively. Major variations were observed in the S1 portion of the S gene of both HEV strains, with only 73/71% amino acid identities compared with those of the two other haemagglutinating coronaviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-10-2411
2002-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/10/0832411a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-10-2411&mimeType=html&fmt=ahah

References

  1. Abraham S., Kienzle T. E., Lapps W., Brian D. A. 1991; Deduced sequence of the bovine coronavirus spike protein and identification of the internal proteolytic cleavage site. Virology 176:296–301
    [Google Scholar]
  2. Andries K., Pensaert M. B. 1980; Virus isolated and immunofluorescence in different organs of pigs infected with hemagglutinating encephlomyelitis virus. American Journal of Veterinary Research 41:215–218
    [Google Scholar]
  3. Boireau P., Cruciere C., Laporte J. 1990; Nucleotide sequence of the glycoprotein S gene of bovine enteric coronavirus and comparison with the S proteins of two mouse hepatitis virus strains. Journal of General Virology 71:487–492
    [Google Scholar]
  4. Boutin M., Sasseville A. M.-J., Dea S. 2001; Detection and identification of hemagglutinating coronaviruses using group-specific single and multiplex RT–PCR. In Proceedings of the X International Symposium of Veterinary Laboratory Diagnostics. Salsomaggiore, Parma, Italy. July 4–7 pp 391–392
    [Google Scholar]
  5. Breslin J. J., Smith L. G., Fuller F. G., Guy J. S. 1999; Sequences analysis of the turkey coronavirus nucleocapsid gene and 3′ untranslated region identifies the virus as a close relative of infectious bronchitis virus. Virus Research 65:187–198
    [Google Scholar]
  6. Cavanagh D., Mawditt K., Sharma M., Drury S. E., Ainsworth H. L., Britton P., Gouch R. E. 2001; Detection of a coronavirus from turkey poults in Europe genetically related to infectious bronchitis virus of chickens. Avian Pathology 30:365–378
    [Google Scholar]
  7. Dea S., Tijssen P. 1989; Antigenic and polypeptide structure of turkey enteric coronaviruses as defined by monoclonal antibodies. Journal of General Virology 70:1725–1741
    [Google Scholar]
  8. Dea S., Verbeek A. J., Tijssen P. 1990; Antigenic and genomic relationships among turkey and bovine enteric coronaviruses. Journal of Virology 64:3112–3118
    [Google Scholar]
  9. Deregt D., Babiuk L. A. 1987; Monoclonal antibodies to bovine coronavirus: characteristics and topographical mapping of neutralizing epitopes on the E2 and E3 glycoproteins. Virology 161:410–420
    [Google Scholar]
  10. Gelinas A., Boutin M., Sasseville A. M.-J., Dea S. 2001; Bovine coronaviruses associated with enteric and respiratory diseases in Canadian dairy cattle display different reactivities to anti-HE monoclonal antibodies and distinct amino acid changes in their HE, S and ns 4·9 protein. Virus Research 76:43–57
    [Google Scholar]
  11. Greig A. S., Bouillant A. M. 1972; Studies on the hemagglutination phenomenon of hemagglutinating encephalomyelitis virus (HEV) of pigs. Canadian Journal of Comparative Medicine 36:366–370
    [Google Scholar]
  12. Hogue B. G., Brian D. A. 1986; Structural proteins of human respiratory coronavirus OC43. Virus Research 5:131–144
    [Google Scholar]
  13. Ismail M. M., Cho K. O., Hasoksuz M., Saif L. J., Saif Y. M. 2002; Antigenic and genomic relatedness of turkey-origin coronaviruses, bovine coronavirus, and infectious bronchitis virus of chickens. Avian Diseases 45:978–984
    [Google Scholar]
  14. Kamahora T., Soe L., Lai M. M. 1989; Sequencing analysis of the nucleocapsid gene and leader RNA of human coronavirus OC43. Virus Research 12:1–9
    [Google Scholar]
  15. Kienzle T. E., Abraham S., Hogue B. G., Brian D. A. 1990; Structure and orientation of expressed bovine coronavirus hemagglutinin–esterase protein. Journal of Virology 64:1834–1838
    [Google Scholar]
  16. King B., Potts B. J., Brian D. A. 1985; Bovine coronavirus hemagglutinin protein. Virus Research 2:53–59
    [Google Scholar]
  17. Lapps W., Hogue B. G., Brian D. A. 1987; Sequence analysis of the bovine coronavirus nucleocapsid and matrix protein genes. Virology 157:47–57
    [Google Scholar]
  18. Lin T. l., Tsai S. C., Wu C. C., Bryan T. A., Thaker H. L., Hooper T., Schrader D. 2002; Characterization of turkey coronaviruses from turkey poults with acute enteritis. Veterinary Microbiology 84:179–186
    [Google Scholar]
  19. Loa C. C., Lin T. L., Wu C. C., Bryan T. A., Thacker H. L., Hooper T., Schrader D. 2000; Detection of antibody to turkey coronavirus by antibody-capture enzyme-linked immunosorbent assay utilizing infectious bronchitis virus antigen. Avian Diseases 44:498–506
    [Google Scholar]
  20. Michaud L., Dea S. 1993; Characterization of monoclonal antibodies to bovine enteric coronavirus and antigenic variability among Quebec isolates. Archives of Virology 131:455–465
    [Google Scholar]
  21. Mounir S., Talbot P. J. 1992; Sequence analysis of the membrane protein gene of human coronavirus OC43 and evidence for O -glycosylation. Journal of General Virology 73:2731–2736
    [Google Scholar]
  22. Mounir S., Talbot P. J. 1993a; Human coronavirus OC43 RNA 4 lacks two open reading frames located downstream of the S gene of bovine coronavirus. Virology 192:355–360
    [Google Scholar]
  23. Mounir S., Talbot P. J. 1993b; Molecular characterization of the S protein gene of human coronavirus OC43. Journal of General Virology 74:1981–1987
    [Google Scholar]
  24. Parker M. D., Yoo D., Cox G. J., Babiuk L. A. 1990a; Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells. Journal of General Virology 71:263–270
    [Google Scholar]
  25. Parker M. D., Yoo D., Babiuk L. A. 1990b; Expression and secretion of the bovine coronavirus hemagglutinin–esterase glycoprotein by insect cells infected with recombinant baculoviruses. Journal of Virology 64:1625–1629
    [Google Scholar]
  26. Pensaert M., Andries K. 1993; Hemagglutinating encephalomyelitis virus. In Diseases of Swine , section 2, Viral Diseases. , 7th edn. pp 268–273 Ames, Iowa: ISU Press;
  27. Spaan W., Cavanagh D., Horzinek M. C. 1988; Coronaviruses: structure and genome expression. Journal of General Virology 69:2939–2952
    [Google Scholar]
  28. Storz J., Stine L., Liem A., Anderson G. A. 1996; Coronavirus isolation from nasal swab samples in cattle with signs of respiratory tract disease after shipping. Journal of the American Veterinary Medical Association 208:1452–1455
    [Google Scholar]
  29. Vautherot J. F., Madelaine M. F., Boireau P., Laporte J. 1992; Bovine coronavirus peplomer glycoproteins: detailed antigenic analyses of S1, S2 and HE. Journal of General Virology 73:1725–1737
    [Google Scholar]
  30. Verbeek A., Tijssen P. 1991; Sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus. Journal of General Virology 72:1659–1666
    [Google Scholar]
  31. Vieler E., Schlapp T., Anders C., Herbst W. 1995; Genomic relationship of porcine hemagglutinating encephalomyelitis virus to bovine coronavirus and human coronavirus OC43 as studied by the use of bovine coronavirus S gene-specific probes. Archives of Virology 140:1215–1223
    [Google Scholar]
  32. Vieler E., Schlapp T., Herbst W. 1996; The region between the M and S genes of porcine haemagglutinating encephalomyelitis virus is highly similar to human coronavirus OC43. Journal of General Virology 77:1443–1447
    [Google Scholar]
  33. Vlasak R., Luytjes W., Leider J., Spaan W., Palese P. 1988; The E3 protein of bovine coronavirus is a receptor-destroying enzyme with acetylesterase activity. Journal of Virology 62:4686–4690
    [Google Scholar]
  34. Von Heijne G. 1986; A new method for predicting signal sequence cleavage amino acid sites. Nucleic Acids Research 14:4683–4690
    [Google Scholar]
  35. Yoo D., Parker M. D., Song J., Cox G. J., Deregt D., Babiuk L. A. 1991; Structural analysis of the conformational domains involved in neutralization of bovine coronavirus using deletion mutants of the spike glycoprotein S1 subunit expressed by recombinant baculoviuses. Virology 183:91–98
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-10-2411
Loading
/content/journal/jgv/10.1099/0022-1317-83-10-2411
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error