1887

Abstract

Lipopeptides which carry the N-terminal moiety tripalmitoyl--glyceryl-cysteinyl-seryl-seryl (PCSS) have been shown to have effective adjuvant and transmembrane carrier properties. To test the ability of these constructs to immunize against simian immunodeficiency virus [(SIV)] infection, rhesus macaques, prescreened for expression of the Mamu-A*01 MHC class I molecule, were immunized at regular intervals with lipopeptides corresponding to known SIV CTL epitopes alone or in combination with multiple antigenic peptides corresponding to neutralizing epitopes. Both humoral and CTL responses were elicited and the monkeys, along with non-immunized control animals, were challenged intravenously with 20 MID of the homologous, uncloned SIV grown in rhesus monkey PBMC. Although none of the monkeys were protected from infection, most demonstrated an anamnestic CTL response with epitope-specific CTL precursor frequencies reaching as high as 1 in 20 total PBMC as measured by limiting dilution CTL assay or 25% of all CD8 T-cells using tetrameric MHC-I/peptide complexes. A significant inverse correlation between the levels of CTLp and the number of infected cells in circulation was observed. However, no such correlation with the plasma viral load (RNA copies/ml) was evident.

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2002-01-01
2020-07-07
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References

  1. Agrawal B., Krantz M. J., Reddish M. A., Longenecker B. M.. 1998; Rapid induction of primary human CD4+ and CD8+ T cell responses against cancer-associated MUC1 peptide epitopes. International Immunology10:1907–1916
    [Google Scholar]
  2. Aichele P., Hengartner H., Zinkernagel R. M., Schulz M.. 1990; Antiviral cytotoxic T-cell response induced by in vivo priming with a free synthetic peptide. Journal of Experimental Medicine171:1815–1820
    [Google Scholar]
  3. Aliprantis A. O., Yang R. B., Mark M. R., Suggett S., Devaux B., Radolf J. D., Klimpel G. R., Godowski P., Zychlinsky A.. 1999; Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2. Science285:736–739
    [Google Scholar]
  4. Allen T. M., Mothe B. R., Sidney J., Jing P., Dzuris J. L., Liebl M. E., Vogel T. U., O′Connor D. H., Wang X., Wussow M. C., Thomson J. A., Altman J. D., Watkins D. I., Sette A.. 2001; CD8+ lymphocytes from simian immunodeficiency virus-infected rhesus macaques recognize 14 different epitopes bound by the major histocompatibility complex class I molecule mamu-A*01: implications for vaccine design and testing. Journal of Virology75:738–749
    [Google Scholar]
  5. Allen T. M., Sidney J., del Guercio M. F., Glickman R. L., Lensmeyer G. L., Wiebe D. A., DeMars R., Pauza C. D., Johnson R. P., Sette A., Watkins D. I.. 1998; Characterization of the peptide binding motif of a rhesus MHC class I molecule (Mamu-A*01) that binds an immunodominant CTL epitope from simian immunodeficiency virus. Journal of Immunology160:6062–6071
    [Google Scholar]
  6. Altman J. D., Moss P. A. H., Goulder P. J. R., Barouch D. H., McHeyzer-Williams M. G., Bell J. I., McMichael A. J., Davis M. M.. 1996; Phenotypic analysis of antigen-specific T lymphocytes. Science274:94–96
    [Google Scholar]
  7. Barouch D. H., Santra S., Schmitz J. E., Kuroda M. J., Fu T. M., Wagner W., Bilska M., Craiu A., Zheng X. X., Krivulka G. R., Beaudry K., Lifton M. A., Nickerson C. E., Trigona W. L., Punt K., Freed D. C., Guan L., Dubey S., Casimiro D., Simon A., Davies M. E., Chastain M., Strom T. B., Gelman R. S., Montefiori D. C., Lewis M. G., Emini E. A., Shiver J. W., Letvin N.. 2000; Control of viremia and prevention of clinical AIDS in rhesus monkeys by cytokine-augmented DNA vaccination. Science290:486–492
    [Google Scholar]
  8. Benichou S., Legrand R., Nakagawa N., Faure T., Traincard F., Vogt G., Dormont D., Tiollais P., Kieny M. P., Madaule P.. 1992; Identification of a neutralizing domain in the external envelope glycoprotein of simian immunodeficiency virus. AIDS Research and Human Retroviruses8:1165–1170
    [Google Scholar]
  9. BenMohamed L., Gras-Masse H., Tartar A., Daubersies P., Brahimi K., Bossus M., Thomas A., Druilhe P.. 1997; Lipopeptide immunization without adjuvant induces potent and long- lasting B, T helper, and cytotoxic T lymphocyte responses against a malaria liver stage antigen in mice and chimpanzees. European Journal of Immunology27:1242–1253
    [Google Scholar]
  10. Beretta A., Grassi F., Pelagi M., Clivio A., Parravicini C., Giovinazzo G., Andronico F., Lopalco L., Verani P., Butto S., Titti F., Rossi G. B., Viale G., Ginelli E., Siccardi A. G.. 1987; HIV env glycoprotein shares a cross-reacting epitope with a surface protein present on activated human monocytes and involved in antigen presentation. European Journal of Immunology17:1793–1798
    [Google Scholar]
  11. Borrow P., Lewicki H., Wei X., Horwitz M. S., Peffer N., Meyers H., Nelson J. A., Gairin J. E., Hahn B. H., Oldstone M. B., Shaw G. M.. 1997; Antiviral pressure exerted by HIV-1-specific cytotoxic T lymphocytes (CTLs) during primary infection demonstrated by rapid selection of CTL escape virus. Nature Medicine3:205–211
    [Google Scholar]
  12. Bourgault I., Venet A., Levy J. P.. 1992; Three epitopic peptides of the simian immunodeficiency virus Nef protein recognized by macaque cytolytic T lymphocytes. Journal of Virology66:750–756
    [Google Scholar]
  13. Brenneman D. E., Westbrook G. L., Fitzgerald S. P., Ennist D. L., Elkins K. L., Ruff M. R., Pert C. B.. 1988; Neuronal cell killing by the envelope protein of HIV and its prevention by vasoactive intestinal peptide. Nature335:639–642
    [Google Scholar]
  14. Defoort J. P., Nardelli B., Huang W., Ho D. D., Tam J. P.. 1992; Macromolecular assemblage in the design of a synthetic AIDS vaccine. Proceedings of the National Academy of Sciences, USA89:3879–3883
    [Google Scholar]
  15. Deres K., Schild H., Wiesmueller K. H., Jung G., Rammensee H. G.. 1989; In vivo priming of virus-specific cytotoxic T lymphocytes with synthetic lipopeptide vaccine. Nature342:561–564
    [Google Scholar]
  16. Donnenberg A. D., Margolick J. B., Polk B. F.. 1989; Limiting dilution analysis of in vivo-activated (IL-2 responsive) peripheral blood lymphocytes in HIV-1-infected subjects. Clinical Immunology and Immunopathology51:91–98
    [Google Scholar]
  17. Eo S. K., Gierynska M., Kamar A. A., Rouse B. T.. 2001; Prime-boost immunization with DNA vaccine: mucosal route of administration changes the rules. Journal of Immunology166:5473–5479
    [Google Scholar]
  18. Evans D. T., O’Connor D. H., Jing P., Dzuris J. L., Sydney J., DaSilva J., Allen T. M., Horton H., Venham J. E., Rudersdorf A., Vogel T., Pauza C. D., Bontrop R. E., DeMars R., Sette A., Hughes A. L., Watkins D. I.. 1999; Virus-specific cytotoxic T-lymphocyte responses select for amino acid variation in SIV Env and Nef. Nature Medicine5:1270–1276
    [Google Scholar]
  19. Evans D. T., Jing P., Allen T. M., O’Connor D. H., Horton H., Venham J. E., Piekarczyk M., Dzuris J., Dykhuzen M., Mitchen J., Rudersdorf R. A., Pauza C. D., Sette A., Bontrop R. E., DeMars R., Watkins D. I.. 2000; Definition of five new simian immunodeficiency virus cytotoxic T-lymphocyte epitopes and their restricting major histocompatibility complex class I molecules: evidence for an influence on disease progression. Journal of Virology74:7400–7410
    [Google Scholar]
  20. Gallichan W. S., Rosenthal K. L.. 1996; Long-lived cytotoxic T lymphocyte memory in mucosal tissues after mucosal but not systemic immunization. Journal of Experimental Medicine184:1879–1890
    [Google Scholar]
  21. Gallimore A., Cranage M., Cook N., Almond N., Bootman J., Rud E., Silvera P., Dennis M., Corcoran T., Stott J., McMichael A., Gotch F.. 1995; Early suppression of SIV replication by CD8+ Nef-specific cytotoxic T cells in vaccinated macaques. Nature Medicine1:1167–1173
    [Google Scholar]
  22. Hanke T., Samuel R. V., Blanchard T. J., Neumann V. C., Allen T. M., Boyson J. E., Sharpe S. A., Cook N., Smith G. L., Watkins D. I., Cranage M. P., McMichael A. J.. 1999; Effective induction of simian immunodeficiency virus-specific cytotoxic T lymphocytes in macaques by using a multiepitope gene and DNA prime- modified vaccinia virus Ankara boost vaccination regimen. Journal of Virology73:7524–7532
    [Google Scholar]
  23. Hart M. K., Weinhold K. J., Scearce R. M., Washburn E. M., Clark C. A., Palker T. J., Haynes B. F.. 1991; Priming of anti-human immunodeficiency virus (HIV) CD8+ cytotoxic T cells in vivo by carrier-free HIV synthetic peptides. Proceedings of the National Academy of Sciences, USA88:9448–9452
    [Google Scholar]
  24. Jordan H. L., Kuroda M. J., Schmitz J. E., Steenbeke T., Forman M. A., Letvin N. L.. 1999; Detection of simian immunodeficiency virus Gag-specific CD8+ T lymphocytes in semen of chronically infected rhesus monkeys by cell staining with a tetrameric major histocompatibility complex class I- peptide complex. Journal of Virology73:4508–4512
    [Google Scholar]
  25. Kast W. M., Roux L., Curren J., Blom H. J. J., Voordouw A. C., Meloen R. H., Kolakofsky D., Melief J. M.. 1991; Protection against lethal Sendai virus infection by in vivo priming of virus-specific cytotoxic T lymphocytes with a free synthetic peptide. Proceedings of the National Academy of Sciences, USA88:2283–2287
    [Google Scholar]
  26. Kent K. A., Rud E., Corcoran T., Powell C., Thiriart C., Collignon C., Stott E. J.. 1992; Identification of two neutralizing and 8 non-neutralizing epitopes on simian immunodeficiency virus envelope using monoclonal antibodies. AIDS Research and Human Retroviruses8:1147–1151
    [Google Scholar]
  27. Kuroda M. J., Schmitz J. E., Barouch D. H., Craiu A., Allen T. M., Sette A., Watkins D. I., Forman M. A., Letvin N. L.. 1998; Analysis of Gag-specific cytotoxic T lymphocytes in simian immunodeficiency virus-infected rhesus monkeys by cell staining with a tetrameric major histocompatibility complex class I–peptide complex. Journal of Experimental Medicine187:1373–1381
    [Google Scholar]
  28. Kuroda M. J., Schmitz J. E., Charini W. A., Nickerson C. E., Lord C. I., Forman M. A., Letvin N. L.. 1999; Comparative analysis of cytotoxic T lymphocytes in lymph nodes and peripheral blood of simian immunodeficiency virus-infected rhesus monkeys. Journal of Virology73:1573–1579
    [Google Scholar]
  29. Lex A., Wiesmueller K. H., Jung G., Bessler W. G.. 1986; A synthetic analogue of Escherichia coli lipoprotein, tripalmitoyl pentapeptide, constitutes a potent immune adjuvant. Journal of Immunology137:2676–2681
    [Google Scholar]
  30. Livingston B. D., Crimi C., Grey H., Ishioka G., Chisari F. V., Fikes J., Chesnut R. W., Sette A.. 1997; The hepatitis B virus-specific CTL responses induced in humans by lipopeptide vaccination are comparable to those elicited by acute viral infection. Journal of Immunology159:1383–1392
    [Google Scholar]
  31. Miller M. D., Yamamoto H., Hughes A. L., Watkins D. I., Letvin N. L.. 1991; Definition of an epitope and MHC class I molecule recognized by Gag-specific cytotoxic T lymphocytes in SIVmac-infected rhesus monkeys. Journal of Immunology147:320–329
    [Google Scholar]
  32. Mortara L., Gras-Masse H., Rommens C., Venet A., Guillet J. G., Bourgault-Villada I.. 1999; Type 1 CD4+ T-cell help is required for induction of antipeptide multispecific cytotoxic T lymphocytes by a lipopeptidic vaccine in rhesus macaques. Journal of Virology73:4447–4451
    [Google Scholar]
  33. Nardelli B., Lu Y. A., Shiu D. R., Delpierre-Defoort C., Profy A. T., Tam J. P.. 1992; A chemically defined synthetic vaccine model for HIV-1. Journal of Immunology148:914–920
    [Google Scholar]
  34. Norley S. G., Kurth R.. 1996; AIDS vaccination. In Concepts in Vaccine Development pp353–558 Edited by Kaufmann S. H. E.. Berlin & New York: Walter de Gruyter;
    [Google Scholar]
  35. Norley S. G., Löwer J., Kurth R.. 1993; Insufficient inactivation of HIV-1 in human cryo poor plasma by β-propriolactone: results from a highly accurate virus detection method. Biologicals21:251–258
    [Google Scholar]
  36. Posnett D. N., McGrath H., Tam J. P.. 1988; A novel method for producing anti-peptide antibodies. Production of site-specific antibodies to the T cell antigen receptor beta-chain. Journal of Biological Chemistry263:1719–1725
    [Google Scholar]
  37. Prass W., Ringsdorf H., Bessler W., Wiesmüller K. H., Jung G.. 1987; Lipopeptides of the N-terminus of Escherichia coli lipoprotein: synthesis, mitogenicity and properties in monolayer experiments. Biochimica et Biophysica Acta900:116–128
    [Google Scholar]
  38. Reiher W. E., Blalock J. E., Brunck T. K.. 1986; Sequence homology between acquired immunodeficiency syndrome virus envelope protein and interleukin 2. Proceedings of the National Academy of Sciences, USA83:9188–9192
    [Google Scholar]
  39. Robinson W. E., Montefiori D. C., Mitchell W. M.. 1987; A human immunodeficiency virus type 1 (HIV-1) infection-enhancing factor in seropositive sera. Biochemical and Biophysical Research Communications149:693–699
    [Google Scholar]
  40. Robinson W. E., Kawamura T., Lake D., Masuho Y., Mitchell W. M., Hersh E. M.. 1990; Antibodies to the primary immunodominant domain of human immunodeficiency virus type 1 (HIV-1) glycoprotein gp41 enhance HIV-1 infection in vitro. Journal of Virology64:5301–5305
    [Google Scholar]
  41. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A.. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science239:487–491
    [Google Scholar]
  42. Sastry K. J., Nehete P. N., Venkatnarayanan S., Morkowski J., Platsoucas C. D., Arlinghaus R. B.. 1992; Rapid in vivo induction of HIV-specific CD8+ cytotoxic T lymphocytes by a 15-amino acid unmodified free peptide from the immunodominant V3-loop of GP120. Virology188:502–509
    [Google Scholar]
  43. Schild H., Deres K., Wiesmueller K. H., Jung G., Rammensee H. G.. 1991; Efficiency of peptides and lipopeptides for in vivo priming of virus-specific cytotoxic T cells. European Journal of Immunology21:2649–2654
    [Google Scholar]
  44. Schlienger K., Montefiori D. C., Mancini M., Riviere Y., Tiollais P., Michel M. L.. 1994; Vaccine-induced neutralizing antibodies directed in part to the simian immunodeficiency virus (SIV) V2 domain were unable to protect rhesus monkeys from SIV experimental challenge. Journal of Virology68:6578–6588
    [Google Scholar]
  45. Seth A., Ourmanov I., Kuroda M. J., Schmitz J. E., Carroll M. W., Wyatt L. S., Moss B., Forman M. A., Hirsch V. M., Letvin N. L.. 1998; Recombinant modified vaccinia virus Ankara-simian immunodeficiency virus gag pol elicits cytotoxic T lymphocytes in rhesus monkeys detected by a major histocompatibility complex class I/peptide tetramer. Proceedings of the National Academy of Sciences, USA95:10112–10116
    [Google Scholar]
  46. Tam J. P.. 1988; Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system. Proceedings of the National Academy of Sciences, USA85:5409–5413
    [Google Scholar]
  47. Tan L. C., Gudgeon N., Annels N. E., Hansasuta P., O’Callaghan C. A., Rowland-Jones S., McMichael A. J., Rickinson A. B., Callan M. F.. 1999; A re-evaluation of the frequency of CD8+ T cells specific for EBV in healthy virus carriers. Journal of Immunology162:1827–1835
    [Google Scholar]
  48. Torres J. V., Malley A., Banapour B., Anderson D. E., Axthelm M. K., Gardner M. B., Benjamini E.. 1993; An epitope on the surface envelope glycoprotein (gp130) of simian immunodeficiency virus (SIVmac) involved in viral neutralization and T cell activation. AIDS Research and Human Retroviruses9:423–430
    [Google Scholar]
  49. Troalen F., Razafindratsita A., Puisieux A., Voeltzel T., Bohuon C., Bellet D., Bidart J. M.. 1990; Structural probing of human lutropin using antibodies raised against synthetic peptides constructed by classical and multiple antigen peptide system approaches. Molecular Immunology27:363
    [Google Scholar]
  50. Veazey R. S., DeMaria M., Chalifoux L. V., Shvetz D. E., Pauley D. R., Knight H. L., Rosenzweig M., Johnson R. P., Desrosiers R. C., Lackner A. A.. 1998; Gastrointestinal tract as a major site of CD4+ T cell depletion and viral replication in SIV infection. Science280:427–431
    [Google Scholar]
  51. Vega M. A., Guigo R., Smith T. F.. 1990; Autoimmune response in AIDS. Nature345:26
    [Google Scholar]
  52. Vitiello A., Ishioka G., Grey H. M., Rose R., Farness P., LaFond R., Yuan L., Chisari F. V., Furze J., Bartholomeuz R.. and others 1995; Development of a lipopeptide-based therapeutic vaccine to treat chronic HBV infection. I. Induction of a primary cytotoxic T lymphocyte response in humans. Journal of Clinical Investigation95:341–349
    [Google Scholar]
  53. Vogel T., Kurth R., Norley S.. 1994; The majority of neutralizing Abs in HIV-1-infected patients recognize linear V3 loop sequences. Studies using HIV-1MN multiple antigenic peptides. Journal of Immunology153:1895–1904
    [Google Scholar]
  54. Vogel T., Norley S., Beer B., Kurth R.. 1995; Rapid screening for Mamu-A1-positive rhesus macaques using a SIVmac Gag peptide-specific cytotoxic T-lymphocyte assay. Immunology84:482–487
    [Google Scholar]
  55. Wang C. Y., Looney D. J., Li M. L., Walfield A. M., Ye J., Hosein B., Tam J. P., Wong-Staal F.. 1991; Long-term high-titer neutralizing activity induced by octameric synthetic HIV-1 antigen. Science254:285–288
    [Google Scholar]
  56. Watanari E., Dietzchold B., Szokan G., Herber-Katz E.. 1987; A synthetic peptide induces long-term protection from lethal infection with herpes simplex virus 2. Journal of Experimental Medicine165:459–470
    [Google Scholar]
  57. Watkins D. I., Kannagi M., Stone M. E., Letvin N. L.. 1988; Major histocompatibility complex class I molecules of nonhuman primates. European Journal of Immunology18:1425–1432
    [Google Scholar]
  58. Wiesmüller K. H., Bessler W., Jung G.. 1983; Synthesis of the mitogenic S-{2,3-Bis(palmitoyloxy)propyl}-N-palmitoylpentapeptide from E. coli lipoprotein. Hoppe-Seyler’s Zeitschrift fuer Physiologische Chemie364:593–606
    [Google Scholar]
  59. Yamamoto H., Miller M. D., Tsubota H., Watkins D. I., Mazzara G. P., Stallard V., Panicali D. L., Aldovini A., Young R. A., Letvin N. L.. 1990; Studies of cloned simian immunodeficiency virus-specific T lymphocytes. Gag-specific cytotoxic T lymphocytes exhibit a restricted epitope specificity. Journal of Immunology144:3385–3391
    [Google Scholar]
  60. Young J. A.. 1988; HIV and HLA similarity. Nature333:215
    [Google Scholar]
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