1887

Abstract

This study describes genetic differences in resistance/susceptibility to pseudorabies virus (PrV) between European Large White and Chinese Meishan pigs, with a mapping of quantitative trait loci (QTL) obtained from a genome-wide scan in F animals. Eighty-nine F pigs were challenged intranasally at 12 weeks with 10 p.f.u. of the wild-type PrV strain NIA-3. For QTL analysis, 85 microsatellite markers, evenly spaced on the 18 porcine autosomes and on the pseudoautosomal region of the X chromosome, were genotyped. All pigs developed clinical signs, i.e. fever, from 3 to 7 days p.i. The pure-bred Large White pigs, the F and three-quarters of the F animals, but none of the Meishan pigs, developed neurological symptoms and died or were euthanized. QTLs for appearance/non-appearance of neurological symptoms were found on chromosomes 9, 5, 6 and 13. They explained 10·6–17·9% of F phenotypic variance. QTL effects for rectal temperature after PrV challenge were found on chromosomes 2, 4, 8, 10, 11 and 16. Effects on chromosomes 9, 10 and 11 were significant on a genome-wide level. The results present chromosomal regions that are associated with presence/absence of neurological symptoms as well as temperature course after intranasal challenge with NIA-3. The QTLs are in proximity to important candidate genes that are assumed to play crucial roles in host defence against PrV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-1-167
2002-01-01
2020-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/1/0830167a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-1-167&mimeType=html&fmt=ahah

References

  1. Baskerville A.. 1981; Aujeszky’s disease: recent advances and current problems. New Zealand Veterinary Journal29:183–185
    [Google Scholar]
  2. Baskerville A., McFerran J. B., Dow C.. 1973; Aujeszky’s disease in pigs. Veterinary Bulletin43:465–480
    [Google Scholar]
  3. Churchill G. A., Doerge R. W.. 1994; Empirical threshold values for quantitative trait mapping. Genetics138:963–971
    [Google Scholar]
  4. Eberle F., Dubreuil P., Mattei M. G., Devilard E., Lopez M.. 1995; The human PRR2 gene, related to the human poliovirus receptor gene (PVR), is the true homolog of the murine MPH gene. Gene159:267–272
    [Google Scholar]
  5. Favoreel H. W., Nauwynck H. J., Halewyck H. M., Van Oostveldt P., Mettenleiter T. C., Pensaert M. B.. 1999; Antibody-induced endocytosis of viral glycoproteins and major histocompatibility complex class I on pseudorabies virus-infected monocytes. Journal of General Virology80:1283–1291
    [Google Scholar]
  6. Geraghty R. J., Krummenacher C., Cohen G. H., Eisenberg R. J., Spear P. G.. 1998; Entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor. Science280:1618–1620
    [Google Scholar]
  7. Green P., Falls K., Crooks S.. 1990; Documentation for Crimap version 2.4 St Louis: Washington University School of Medicine. Polykopie;
    [Google Scholar]
  8. Grob P., Schijns V. E. C. J., Van den Broek M. F., Cox S. P. J., Ackermann M., Suter M.. 1999; Role of the individual interferon systems and specific immunity in mice in controlling systemic dissemination of attenuated pseudorabies virus infection. Journal of Virology73:4748–4754
    [Google Scholar]
  9. Haley C. S., Knott S. A., Elsen J. M.. 1994; Mapping quantitative trait loci in crosses between outbred lines using least squares. Genetics136:1195–1207
    [Google Scholar]
  10. Hessing M. J., Scheepens C. J., Schouten W. G., Tielen M. J., Wiepkema P. R.. 1994; Social rank and disease susceptibility in pigs. Veterinary Immunology and Immunopathology43:373–387
    [Google Scholar]
  11. Hessing M. J., Coenen G. J., Vaiman M., Renard C.. 1995; Individual differences in cell-mediated and humoral immunity in pigs. Veterinary Immunology and Immunopathology45:97–113
    [Google Scholar]
  12. Husak P. J., Kuo T., Enquist L. W.. 2000; Pseudorabies virus membrane proteins gI and gE facilitate anterograde spread of infection in projection-specific neurons in the rat. Journal of Virology74:10975–10983
    [Google Scholar]
  13. Karger A., Mettenleiter T. C.. 1996; Identification of cell surface molecules that interact with pseudorabies virus. Journal of Virology70:2138–2145
    [Google Scholar]
  14. Keats B. J., Sherman S. L., Morton N. E., Robson E. B., Buetow K. H., Cartwright P. E., Chakravarti A., Francke U., Green P. P., Ott J.. 1991; Guidelines for human linkage maps: an international system for human linkage maps (ISLM, 1990). Genomics9:557–560
    [Google Scholar]
  15. Kimman T. G., de Wind N., Oei-Lie N., Pol J. M. A., Berns A. J. M., Gielkens A. L. J.. 1992; Contribution of single genes within the unique short region of Aujeszky’s disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. Journal of General Virology73:243–251
    [Google Scholar]
  16. Kluge J. P., Beran G. W., Hill H. T., Platt K. B.. 1999; Pseudorabies. In Diseases of Swine pp233–246 Edited by Straw B. E., Dállaire S., Mengeling W. L., Taylor D. J.. Oxford: Blackwell Science;
    [Google Scholar]
  17. Kritas S. K., Pensaert M. B., Mettenleiter T. C.. 1994; Role of envelope glycoproteins gI, gp63 and gIII in the invasion and spread of Aujeszky’s disease virus in the olfactory nervous pathway of the pig. Journal of General Virology75:2319–2327
    [Google Scholar]
  18. McFerran J. B., Dow C.. 1975; Studies on immunisation of pigs with the Bartha strain of Aujeszky’s disease virus. Research in Veterinary Science19:17–22
    [Google Scholar]
  19. Meeker D. L., Rothschild M. F., Christian L. L., Warner C. M., Hill H. T.. 1987a; Genetic control of immune response to pseudorabies and atrophic rhinitis vaccines: I. Heterosis, general combining ability and relationship to growth and backfat. Journal of Animal Science64:407–413
    [Google Scholar]
  20. Meeker D. L., Rothschild M. F., Christian L. L., Warner C. M., Hill H. T.. 1987b; Genetic control of immune response to pseudorabies and atrophic rhinitis vaccines: II. Comparison of additive direct and maternal genetic effects. Journal of Animal Science64:414–419
    [Google Scholar]
  21. Mettenleiter T. C.. 2000; Aujeszky’s disease (pseudorabies) virus: the virus and molecular pathogenesis – state of the art, June 1999. Veterinary Research31:99–115
    [Google Scholar]
  22. Rohrer G. A., Alexander L. J., Hu Z., Smith T. P., Keele J. W., Beattie C. W.. 1996; A comprehensive map of the porcine genome. Genome Research6:371–391
    [Google Scholar]
  23. Rothschild M. F., Hill H. T., Christian L. L., Warner C. M.. 1984; Genetic differences in serum-neutralization titers of pigs after vaccination with pseudorabies modified live-virus vaccine. American Journal of Veterinary Research45:1216–1218
    [Google Scholar]
  24. Sin J. I., Kim J. J., Arnold R. L., Shroff K. E., McCallus D., Pachuk C., McElhiney S. P., Wolf M. W., Pompa-de Bruin S. J., Higgins T. J., Ciccarelli R. B., Weiner D. B.. 1999; IL-12 gene as a DNA vaccine adjuvant in a herpes mouse model: IL-12 enhances Th1-type CD4+ T cell-mediated protective immunity against herpes simplex virus-2 challenge. Journal of Immunology162:2912–2921
    [Google Scholar]
  25. Zuckermann F. A.. 2000; Aujeszky’s disease virus: opportunities and challenges. Veterinary Research31:121–131
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-1-167
Loading
/content/journal/jgv/10.1099/0022-1317-83-1-167
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error