1887

Abstract

Reverse genetics technology offers the possibility to study the influence of particular amino acids of infectious bursal disease virus (IBDV) on adaptation to tissue culture. Genomic segments A and B of the very virulent (vv) IBDV field strain UK661 were completely cloned and sequenced, and the strain was rescued from full-length cDNA copies of both segments (UK661rev). Using site-directed mutagenesis, alteration of a single amino acid in the segment A-encoded VP2 (A284T) resulted in a limited capacity of UK661 to replicate in tissue culture. Additional alteration of a second amino acid (Q253H) increased replication efficiency in tissue culture. The second mutant (UK661-Q253H-A284T) was used to infect chickens and results were compared with UK661 and UK661rev. Whereas UK661 and UK661rev induced 100% morbidity and 50–80% mortality, UK661-Q253H-A284T proved to be strikingly attenuated, producing neither morbidity nor mortality. Moreover, UK661-Q253H-A284T-infected animals were protected from challenge infection. Thus, alteration of two specific amino acids in the VP2 region of IBDV resulted in tissue culture adaptation and attenuation in chickens of vvIBDV. The data demonstrate that VP2 plays a decisive role in pathogenicity of IBDV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-1-121
2002-01-01
2020-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/1/0830121a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-1-121&mimeType=html&fmt=ahah

References

  1. Bayliss, C. D., Spies, U., Shaw, K., Peters, R. W., Papageorgiou, A., Müller, H. & Boursnell, M. E. G. ( 1990; ). A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2. Journal of General Virology 71, 1303-1311.[CrossRef]
    [Google Scholar]
  2. Birghan, C., Mundt, E. & Gorbalenya, A. E. ( 2000; ). A non-canonical Lon proteinase deficient of the ATPase domain employs the Ser–Lys catalytic dyad to impose broad control over the life cycle of a double-stranded RNA virus. EMBO Journal 19, 114-123.[CrossRef]
    [Google Scholar]
  3. Boot, H. J., ter Huurne, A. A., Peeters, B. P. & Gielkens, A. L. ( 1999; ). Efficient rescue of infectious bursal disease virus from cloned cDNA: evidence for involvement of the 3′-terminal sequence in genome replication. Virology 265, 330-341.[CrossRef]
    [Google Scholar]
  4. Boot, H. J., ter Huurne, A. A., Hoekman, A. J. W., Peeters, B. P. & Gielkens, A. L. ( 2000; ). Rescue of very virulent and mosaic infectious bursal disease virus from cloned cDNA: VP2 is not the sole determinant of the very virulent phenotype. Journal of Virology 74, 6701-6711.[CrossRef]
    [Google Scholar]
  5. Box, P. ( 1989; ). High maternal antibodies help chicks beat virulent strains. World Poultry 53, 17-19.
    [Google Scholar]
  6. Brown, M. D. & Skinner, M. A. ( 1996; ). Coding sequences of both genome segments of a European ’very virulent’ infectious bursal disease virus. Virus Research 40, 1-15.[CrossRef]
    [Google Scholar]
  7. Bygrave, A. C. & Faragher, J. T. ( 1970; ). Mortality associated with Gumboro disease. Veterinary Record 86, 758-759.
    [Google Scholar]
  8. Chettle, N., Stuart, J. C. & Wyeth, P. J. ( 1989; ). Outbreak of virulent infectious bursal disease in East Anglia. Veterinary Record 125, 271-272.[CrossRef]
    [Google Scholar]
  9. Cosgrove, A. S. ( 1962; ). An apparently new disease of chickens – avian nephrosis. Avian Diseases 6, 385-389.[CrossRef]
    [Google Scholar]
  10. Cursiefen, D., Käufer, I. & Becht, H. ( 1979; ). Loss of virulence in a small plaque mutant of the infectious bursal disease virus. Archives of Virology 59, 39-46.[CrossRef]
    [Google Scholar]
  11. Di Fabio, J., Rossini, L. I., Eterradossi, N., Toquin, M. D. & Gardin, Y. ( 1999; ). European-like pathogenic infectious bursal disease viruses in Brazil. Veterinary Record 145, 203-204.
    [Google Scholar]
  12. Eterradossi, N., Picault, J. P., Drouin, P., Guittet, M., L’Hospitalier, R. & Bennejean, G. ( 1992; ). Pathogenicity and preliminary antigenic characterization of six infectious bursal disease virus strains isolated in France from acute outbreaks. Journal of Veterinary Medicine B 39, 683-691.[CrossRef]
    [Google Scholar]
  13. Eterradossi, N., Arnauld, C., Tekaia, F., Toquin, D., Le Coq, H., Rivallan, G., Guittet, M., Domenech, J., van den Berg, T. P. & Skinner, M. A. ( 1999; ). Antigenic and genetic relationship between European very virulent infectious bursal disease viruses and an early West African isolate. Avian Pathology 28, 36-46.[CrossRef]
    [Google Scholar]
  14. Hassan, M. K., Nielsen, C. K., Ward, L. A., Jackwood, D. J. & Saif, Y. M. ( 1996; ). Antigenicity, pathogenicity, and immunogenicity of small and large plaque infectious bursal disease virus clones. Avian Diseases 40, 832-836.[CrossRef]
    [Google Scholar]
  15. Kunkel, T. A., Roberts, J. D. & Zakour, R. A. ( 1987; ). Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods in Enzymology 154, 367-382.
    [Google Scholar]
  16. Lange, H., Müller, H., Käufer, I. & Becht, H. ( 1987; ). Pathogenic and structural properties of wild type infectious bursal disease virus (IBDV) and virus grown in vitro. Archives of Virology 92, 187-196.[CrossRef]
    [Google Scholar]
  17. Leong, J. C., Brown, D., Dobos, P., Kibenge, F. S. B., Ludert, J. E., Müller, H., Mundt, E. & Nicholson, B. ( 2000; ). Family Birnaviridae. In Virus Taxonomy. Seventh Report of the International Committee on the Taxonomy of Viruses , pp. 481-490. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego:Academic Press.
  18. Lim, B. L., Cao, Y., Yu, T. & Mo, C.-W. ( 1999; ). Adaption of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2. Journal of Virology 73, 2854-2862.
    [Google Scholar]
  19. McFerran, J. B., McNulty, M. S., Killop, E. R., Connor, T. J., McCracken, R. M., Collins, P. S. & Allan, G. M. ( 1980; ). Isolation and serological studies with infectious bursal disease virus from fowl, turkeys and ducks: demonstration of a second serotype. Avian Pathology 9, 395-404.[CrossRef]
    [Google Scholar]
  20. Müller, H., Lange, H. & Becht, H. ( 1986; ). Formation, characterization and interfering capacity of a small plaque mutant and of incomplete virus particles of infectious bursal disease virus. Virus Research 4, 297-309.[CrossRef]
    [Google Scholar]
  21. Mundt, E. ( 1999; ). Tissue culture infectivity of different strains of infectious bursal disease virus is determined by distinct amino acids in VP2. Journal of General Virology 80, 2067-2076.
    [Google Scholar]
  22. Mundt, E. & Müller, H. ( 1995; ). Complete nucleotide sequences of 5′- and 3′-noncoding regions of both genome segments of different strains of infectious bursal disease virus. Virology 209, 209-218.
    [Google Scholar]
  23. Mundt, E. & Vakharia, V. N. ( 1996; ). Synthetic transcripts of double-stranded birnavirus genome are infectious. Proceedings of the National Academic of Sciences, USA 93, 11131-11136.[CrossRef]
    [Google Scholar]
  24. Mundt, E., Beyer, J. & Müller, H. ( 1995; ). Identification of a novel viral protein in infectious bursal disease virus-infected cells. Journal of General Virology 76, 437-443.[CrossRef]
    [Google Scholar]
  25. Nakamura, T., Otaki, Y. & Nunoya, T. ( 1992; ). Immunsuppressive effect of a highly virulent infectious bursal disease virus isolated in Japan. Avian Diseases 36, 891-896.[CrossRef]
    [Google Scholar]
  26. Öppling, V., Müller, H. & Becht, H. ( 1991; ). The structural polypeptide VP3 of infectious bursal disease virus carries group- and serotype-specific epitopes. Journal of General Virology 72, 2275-2278.[CrossRef]
    [Google Scholar]
  27. Schröder, A., van Loon, A. A. W. M., Goovaerts, D. & Mundt, E. ( 2000; ). Chimeras in noncoding regions between serotypes I and II of segment A of infectious bursal disease virus are viable and show pathogenic phenotype in chickens. Journal of General Virology 81, 533-540.
    [Google Scholar]
  28. Snyder, D. B., Vakharia, V. N. & Savage, P. K. ( 1992; ). Naturally occurring-neutralizing monoclonal antibody escape variants define the epidemiology of infectious bursal disease virus in the United States. Archives of Virology 127, 89-101.[CrossRef]
    [Google Scholar]
  29. Spies, U., Müller, H. & Becht, H. ( 1987; ). Properties of RNA polymerase activity associated with infectious bursal disease virus and characterization of its reaction products. Virus Research 8, 127-140.[CrossRef]
    [Google Scholar]
  30. Spies, U., Müller, H. & Becht, H. ( 1989; ). Nucleotide sequence of infectious bursal disease virus segment A delineates two major open reading frames. Nucleic Acids Research 17, 7982.[CrossRef]
    [Google Scholar]
  31. van den Berg, T. P., Gonze, M. & Meulemans, G. ( 1991; ). Acute infectious bursal disease in poultry: isolation and characterization of a highly virulent strain. Avian Pathology 20, 133-143.[CrossRef]
    [Google Scholar]
  32. van Loon, A. A. W. M., Lütticken, D. & Snyder, D. B. (1994). Rapid quantification of infectious bursal disease (IBD) challenge, field or vaccine virus strains. Proceedings of the International Symposium on Infectious Bursal Disease and Chicken Infectious Anaemia, pp. 179–187. World Veterinary Poultry Association and Institut für Geflügelkrankheiten, Giessen, Germany, 21–24 June 1994.
  33. Yamaguchi, T., Kondo, T., Inoshima, Y., Ogawa, M., Miyoshi, M., Yanai, T., Masegi, T., Fukushi, H. & Hirai, K. ( 1996a; ). In vitro attenuation of highly virulent infectious bursal disease virus: some characteristics of attenuated strains. Avian Diseases 40, 501-509.[CrossRef]
    [Google Scholar]
  34. Yamaguchi, T., Ogawa, M., Inoshima, Y., Miyoshi, M., Fukushi, H. & Hirai, K. ( 1996b; ). Identification of sequence changes responsible for the attenuation of highly virulent infectious bursal disease virus. Virology 223, 219-223.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-1-121
Loading
/content/journal/jgv/10.1099/0022-1317-83-1-121
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error