A physical map of the genome of Adoxophyes orana granulovirus (AoGV) was constructed for the restriction enzymes BamHI, BglII, EcoRI, PstI and SacI using restriction endonuclease analysis and DNA hybridization techniques. This enabled the size of the AoGV genome to be estimated at 100·9 kbp. A plasmid library covering 99·9% of the AoGV genome was constructed using five restriction enzymes. The ecdysteroid UDP-glucosyltransferase gene (egt) was located by hybridization with the egt gene of Cydia pomonella granulovirus. The sequence of 6000 bp of the egt region is presented and compared to the equivalent area in other GVs. Database searches showed that this region contained eight open reading frames (ORFs) similar to the baculovirus genes egt, granulin, pk-1, me53 and four ORFs of Xestia c-nigrum granulovirus (ORF 178, ORF 2, ORF 7 and ORF 8). The egt gene was shown to encode an active EGT using an EGT assay. Phylogenetic trees of the granulovirus genes egt, granulin, pk-1 and me53 were constructed using maximum parsimony and distance analyses. These analyses indicated that AoGV genes may be more closely related to other tortricid-infecting GVs than to GVs that infect other lepidopteran families.
BaylisS. A.,
BanhamA. H.,
VydelingumS.,
DixonL. K.,
SmithG. L.1993; African swine fever virus encodes a serine protein kinase which is packaged into virions. Journal of Virology 67:4549–4556
BischoffD. S.,
SlavicekJ. M.1994; Identification and characterization of a protein kinase gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus. Journal of Virology 68:1728–1736
CrookN. E.,
JamesJ. D.,
SmithI. R. L.,
WinstanleyD.1997; Comprehensive physical map of the Cydia pomonella granulovirus genome and sequence analysis of the granulin gene region. Journal of General Virology 78:965–974
CrossJ. V.,
SolomonM. G.,
ChandlerD.,
JarrettP.,
RichardsonP. N.,
WinstanleyD.,
BathonH.,
HuberJ.,
KellerB.,
LangenbruchG. A.,
ZimmermanG.1999; Biocontrol of pests of apples and pears in northern and central Europe. 1. Microbial agents and nematodes. Biocontrol Science and Technology 9:125–149
FlipsenJ. T. M.,
MansR. M. W.,
KleefsmanA. W. F.,
Knebel-MörsdorfD.,
VlakJ. M.1995; Deletion of the baculovirus ecdysteroid UDP-glucosyltransferase gene induces early degeneration of malpighian tubules in infected insects. Journal of Virology 69:7380–7381
FreedmanL. P.,
LuisiB. F.,
KorszunZ. R.,
BasavappaR.,
SiglerP. B.,
YamamotoK. R.1988; The function and structure of the metal co-ordination sites within the glucocorticoid receptor DNA-binding domain. Nature 334:543–546
GuennelonG.,
AudemardH.,
FremondJ. C.,
El Idrissi AmmariM. A.1981; Progrès réalisés dans l’elevage permanent du Carpocapse ( Laspeyresia pomonella L.) sur milieu artificiel. Agronomie 1:59–64
HonmaK.1970; Morphological difference of the smaller tea tortrix, Adoxophyes orana , in Japan. Japanese Journal of Applied Entomology and Zoology 14:89–94
HuZ. H.,
BroerR.,
WesterlakenJ.,
MartensJ. W. M.,
JinF.,
JehleJ. A.,
WangL. M.,
VlakJ. M.1997; Characterization of the ecdysteroid UDP-glucosyltransferase gene of a single nucleocapsid nucleopolyhedrovirus of Buzura suppressaria. Virus Research4791–97
IJkelW. F. J.,
van StrienE. A.,
HeldensJ. G. M.,
BroerR.,
ZuidemaD.,
GoldbachR. W.,
VlakJ. M.1999; Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. Journal of General Virology 80:3289–3304
JehleJ. A.,
BackhausH.1994; The granulin gene region of Cryptophlebia leucotreta granulosis virus: sequence analysis and phylogenetic considerations. Journal of General Virology 75:3667–3671
Knebel-MörsdorfD.,
KremerA.,
JahnelF.1993; Baculovirus gene ME53, which contains a putative zinc finger motif, is one of the major early-transcribed genes. Journal of Virology 67:753–758
KuzioJ.,
PearsonM. N.,
HarwoodS. H.,
FunkC. J.,
EvansJ. T.,
SlavicekJ. M.,
RohrmannG. F.1999; Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar. Virology25317–34
MurphyF. A.,
FauquetC. M.,
BishopD. H. L.,
GhabrialS. A.,
JarvisA. W.,
MartelliG. P.,
MayoM. A.,
SummersM. D.
(editors) 1995; Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses Vienna & New York: Springer-Verlag;
O’ReillyD. R.,
BrownM. R.,
MillerL. K.1992; Alteration of ecdysteroid metabolism due to baculovirus infection of the fall armyworm Spodoptera frugiperda – host ecdysteroids are conjugated with galactose. Insect Biochemistry and Molecular Biology 22:313–320
O’ReillyD. R.,
HailsR. S.,
KellyT. J.1998; The impact of host developmental status on baculovirus replication. Journal of Invertebrate Pathology 72:269–275
ReillyL. M.,
GuarinoL. A.1994; The PK-1 gene of Autographa californica multinucleocapsid nuclear polyhedrosis virus encodes a protein kinase. Journal of General Virology 75:2999–3006
SambrookJ.,
FritschE. F.,
ManiatisT.1989Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
SangerF.,
Nicklen & CoulsenA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences USA 74:5463–5467
SmithI. R. L.,
CrookN. E.1988b; Physical maps of the genomes of four variants of Artogeia rapae granulosis virus. Journal of General Virology 69:1741–1747
TahaA.,
Nour-el-DinA.,
CroizierL.,
López FerberM. C.,
CroizierG.2000; Comparative analysis of the granulin regions of the Phthorimaea operculella and Spodoptera littoralis granuloviruses. Virus Genes 21:147–155
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
VlakJ. M.,
SmithG. E.1982; Orientation of the genome of Autographa californica nuclear polyhedrosis virus: a proposal. Journal of Virology 41:1118–1121
YamadaH.,
OnoN.1973; A granulosis virus, possible biological agent for control of Adoxophyes orana (Lepidoptera: Tortricidae) in apple orchards. Journal of Invertebrate Pathology 21:144–148
ZhangC. X.,
HuC.,
WuX. F.1998; Studies on the nucleotide sequence, transcription and deletion analysis of the BmNPV protein kinase gene. Acta Biochimica et Biophysica Sinica 30:185–190