1887

Abstract

The multicapsid nucleopolyhedrovirus (AcMNPV) gene was found previously to be necessary to support optimal levels of transient expression from an AcMNPV late promoter. The gene is unusual in that it overlaps both upstream () and downstream () genes. In this study, the expression and cellular localization of LEF-11 were examined. The transcripts were detected from 4 to 36 h post-infection (p.i.). The 1·5 kb mRNA initiates 196 nt upstream of the translation initiation codon, within the upstream gene. This relatively long 5′ upstream region encodes a potential small upstream open reading frame (ORF) of 58 amino acids that overlaps the ORF. The 3′ end of the mRNA was mapped as co-terminal with mRNAs from the downstream gene. Using affinity purified anti-LEF-11 antibodies, levels of LEF-11 expression were found to be maximal between approximately 8 and 24 h p.i., although LEF-11 could be detected as late as 72 h p.i. Using immunofluorescence microscopy, it was determined that LEF-11 localized to dense regions of infected cell nuclei, consistent with its role as a possible late transcription factor.

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2001-09-01
2024-03-29
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