1887

Abstract

The multicapsid nucleopolyhedrovirus (AcMNPV) gene was found previously to be necessary to support optimal levels of transient expression from an AcMNPV late promoter. The gene is unusual in that it overlaps both upstream () and downstream () genes. In this study, the expression and cellular localization of LEF-11 were examined. The transcripts were detected from 4 to 36 h post-infection (p.i.). The 1·5 kb mRNA initiates 196 nt upstream of the translation initiation codon, within the upstream gene. This relatively long 5′ upstream region encodes a potential small upstream open reading frame (ORF) of 58 amino acids that overlaps the ORF. The 3′ end of the mRNA was mapped as co-terminal with mRNAs from the downstream gene. Using affinity purified anti-LEF-11 antibodies, levels of LEF-11 expression were found to be maximal between approximately 8 and 24 h p.i., although LEF-11 could be detected as late as 72 h p.i. Using immunofluorescence microscopy, it was determined that LEF-11 localized to dense regions of infected cell nuclei, consistent with its role as a possible late transcription factor.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-9-2289
2001-09-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/9/0822289a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-9-2289&mimeType=html&fmt=ahah

References

  1. Ayres, M. D., Howard, S. C., Kuzio, J., Lopez-Ferber, M. & Possee, R. D. ( 1994; ). The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202, 586-605.[CrossRef]
    [Google Scholar]
  2. Baklanov, M. M., Golikova, L. N. & Malygin, E. G. ( 1996; ). Effect on DNA transcription of nucleotide sequences upstream to T7 promoter. Nucleic Acids Research 24, 3659-3660.[CrossRef]
    [Google Scholar]
  3. Blissard, G. W. & Rohrmann, G. F. ( 1989; ). Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170, 537-555.[CrossRef]
    [Google Scholar]
  4. Chang, M.-J. & Blissard, G. W. ( 1997; ). Baculovirus gp64 gene expression: negative regulation by a minicistron. Journal of Virology 71, 7448-7460.
    [Google Scholar]
  5. Friesen, P. D. ( 1997; ). Regulation of baculovirus early gene expression. In The Baculoviruses , pp. 141-166. Edited by L. K. Miller. New York:Plenum.
  6. Fuchs, L. Y., Woods, M. S. & Weaver, R. F. ( 1983; ). Viral transcription during Autographa californica nuclear polyhedrosis virus infection: a novel RNA polymerase induced in infected Spodoptera frugiperda cells. Journal of Virology 48, 641-646.
    [Google Scholar]
  7. Glocker, B., Hoopes, R. R.Jr, Hodges, L. & Rohrmann, G. F. ( 1993; ). In vitro transcription from baculovirus late gene promoters: accurate mRNA initiation by nuclear extracts prepared from infected Spodoptera frugiperda cells. Journal of Virology 67, 3771-3776.
    [Google Scholar]
  8. Gross, C. H. & Shuman, S. ( 1998; ). RNA 5′-triphosphatase, nucleoside triphosphatase, and guanylyltransferase activities of baculovirus LEF-4 protein. Journal of Virology 72, 10020-10028.
    [Google Scholar]
  9. Grula, M. A., Buller, P. L. & Weaver, R. F. ( 1981; ). α-Amanitin-resistant viral RNA synthesis in nuclei isolated from nuclear polyhedrosis virus-infected Heliothis zea larvae and Spodoptera frugiperda cells. Journal of Virology 38, 916-921.
    [Google Scholar]
  10. Guarino, L. A. & Smith, M. W. ( 1990; ). Nucleotide sequence and characterization of the 39k gene region of Autographa californica nuclear polyhedrosis virus. Virology 179, 1-8.[CrossRef]
    [Google Scholar]
  11. Guarino, L. A. & Smith, M. W. ( 1992; ). Regulation of delayed-early gene transcription by dual TATA boxes. Journal of Virology 66, 3733-3739.
    [Google Scholar]
  12. Guarino, L. A., Jin, J. & Dong, W. ( 1998a; ). Guanylyltransferase activity of the LEF-4 subunit of baculovirus RNA polymerase. Journal of Virology 72, 10003-10010.
    [Google Scholar]
  13. Guarino, L. A., Xu, B., Jin, J. & Dong, W. ( 1998b; ). A virus-encoded RNA polymerase purified from baculovirus-infected cells. Journal of Virology 72, 7985-7991.
    [Google Scholar]
  14. Huh, N. E. & Weaver, R. F. ( 1990; ). Categorizing some early and late transcripts directed by the Autographa californica nuclear polyhedrosis virus. Journal of General Virology 71, 2195-2200.[CrossRef]
    [Google Scholar]
  15. Jin, J., Dong, W. & Guarino, L. A. ( 1998; ). The LEF-4 subunit of baculovirus RNA polymerase has RNA 5′-triphosphatase and ATPase activities. Journal of Virology 72, 10011-10019.
    [Google Scholar]
  16. Li, L., Harwood, S. H. & Rohrmann, G. F. ( 1999; ). Identification of additional genes that influence baculovirus late gene expression. Virology 255, 9-19.[CrossRef]
    [Google Scholar]
  17. Lu, A. & Carstens, E. B. ( 1992; ). Transcription analysis of the EcoRI D region of the baculovirus Autographa californica nuclear polyhedrosis virus identifies an early 4-kilobase RNA encoding the essential p143 gene. Journal of Virology 66, 655-663.
    [Google Scholar]
  18. Lu, A. & Miller, L. K. ( 1994; ). Identification of three late expression factor genes within the 33·8- to 43·4-map-unit region of Autographa californica nuclear polyhedrosis virus. Journal of Virology 68, 6710-6718.
    [Google Scholar]
  19. Lu, A. & Miller, L. K. ( 1995; ). The roles of eighteen baculovirus late expression factor genes in transcription and DNA replication. Journal of Virology 69, 975-982.
    [Google Scholar]
  20. Lu, A. & Miller, L. K. ( 1997; ). Regulation of baculovirus late and very late gene expression. In The Baculoviruses , pp. 193-216. Edited by L. K. Miller. New York:Plenum.
  21. Monsma, S. A. & Wolfner, M. F. ( 1988; ). Structure and expression of a Drosophila male accessory gland gene whose product resembles a peptide pheromone precursor. Genes & Development 2, 1063-1073.[CrossRef]
    [Google Scholar]
  22. Okano, K., Mikhailov, V. S. & Maeda, S. ( 1999; ). Colocalization of baculovirus IE-1 and two DNA-binding proteins, DBP and LEF-3, to viral replication factories. Journal of Virology 73, 110-119.
    [Google Scholar]
  23. Passarelli, A. L. & Miller, L. K. ( 1993; ). Identification and characterization of lef-1, a baculovirus gene involved in late and very late gene expression. Journal of Virology 67, 3481-3488.
    [Google Scholar]
  24. Passarelli, A. L., Todd, J. W. & Miller, L. K. ( 1994; ). A baculovirus gene involved in late gene expression predicts a large polypeptide with a conserved motif of RNA polymerases. Journal of Virology 68, 4673-4678.
    [Google Scholar]
  25. Rapp, J. C., Wilson, J. A. & Miller, L. K. ( 1998; ). Nineteen baculovirus open reading frames, including LEF-12, support late gene expression. Journal of Virology 72, 10197-10206.
    [Google Scholar]
  26. Todd, J. W., Passarelli, A. L. & Miller, L. K. ( 1995; ). Eighteen baculovirus genes, including lef-11, p35, 39K, and p47, support late gene expression. Journal of Virology 69, 968-974.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-9-2289
Loading
/content/journal/jgv/10.1099/0022-1317-82-9-2289
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error