1887

Abstract

To study the neutralizing epitopes of influenza B virus Victoria group strains, two monoclonal antibodies (MAbs) were used to select antigenic variants of the virus. MAbs 10B8 and 8E6 were found to react with B/Victoria group strains in three tests, peroxidase–antiperoxidase staining, haemagglutination inhibition and neutralization tests; no reactivity with B/Yamagata group strains was observed. Analysis of the deduced amino acid sequences of 10B8-induced variants identified a single amino acid deletion at residue 165 or 170, as well as a single amino acid substitution at residues 164 (Asp→Tyr), 165 (Asn→Ser or Thr) or 203 (Lys→Thr or Asn). A single amino acid substitution at residue 241 (Pro→Ser) was observed in 8E6-induced variants. Three-dimensional analysis showed that the epitopes for both MAbs were situated in close proximity to each other. Since B/Yamagata group strains are characterized by amino acid deletions at residues 164–166, the epitope for MAb 10B8 is strictly specific for B/Victoria group strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-9-2169
2001-09-01
2019-09-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/9/0822169a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-9-2169&mimeType=html&fmt=ahah

References

  1. Berton, M. T. & Webster, R. G. ( 1985; ). The antigenic structure of the influenza B virus hemagglutinin: operational and topological mapping with monoclonal antibodies. Virology 143, 583-594.[CrossRef]
    [Google Scholar]
  2. Berton, M. T., Naeve, C. W. & Webster, R. G. ( 1984; ). Antigenic structure of the influenza B virus hemagglutinin: nucleotide sequence analysis of antigenic variants selected with monoclonal antibodies. Journal of Virology 52, 919-927.
    [Google Scholar]
  3. Cleveland, S. M., Taylor, H. P. & Dimmock, N. J. ( 1997; ). Selection of neutralizing antibody escape mutants with type A influenza virus HA-specific polyclonal antisera: possible significance for antigenic drift. Epidemiology and Infection 118, 149-154.[CrossRef]
    [Google Scholar]
  4. Daniels, P. S., Jeffries, S., Yates, P., Schild, G. C., Rogers, G. N., Paulson, J. C., Wharton, S. A., Douglas, A. R., Skehel, J. J. & Wiley, D. C. ( 1987; ). The receptor-binding and membrane-fusion properties of influenza virus variants selected using antihaemagglutinin monoclonal antibodies. EMBO Journal 6, 1459-1465.
    [Google Scholar]
  5. Kanegae, Y., Sugita, S., Endo, A., Ishida, M., Senya, S., Osako, K., Nerome, K. & Oya, A. ( 1990; ). Evolutionary pattern of the hemagglutinin gene of influenza B viruses isolated in Japan: cocirculating lineages in the same epidemic season. Journal of Virology 64, 2860-2865.
    [Google Scholar]
  6. Krystal, M., Elliott, R. M., Benz, E. W.Jr, Young, J. F. & Palese, P. ( 1982; ). Evolution of influenza A and B viruses: conservation of structural features in the hemagglutinin genes. Proceedings of the National Academy of Sciences, USA 79, 4800-4804.[CrossRef]
    [Google Scholar]
  7. Lambkin, R., McLain, L., Jones, S. E., Aldridge, S. L. & Dimmock, N. J. ( 1994; ). Neutralization escape mutants of type A influenza virus are readily selected by antisera from mice immunized with whole virus: a possible mechanism for antigenic drift. Journal of General Virology 75, 3493-3502.[CrossRef]
    [Google Scholar]
  8. Lindstrom, S. E., Hiromoto, Y., Nishimura, H., Saito, T., Nerome, R. & Nerome, K. ( 1999; ). Comparative analysis of evolutionary mechanisms of the hemagglutinin and three internal protein genes of influenza B virus: multiple cocirculating lineages and frequent reassortment of the NP, M, and NS genes. Journal of Virology 73, 4413-4426.
    [Google Scholar]
  9. Luoh, S.-M., McGregor, M. W. & Hinshaw, V. S. ( 1992; ). Hemagglutinin mutations related to antigenic variation in H1 swine influenza viruses. Journal of Virology 66, 1066-1073.
    [Google Scholar]
  10. McCullers, J. A., Wang, G. C., He, S. & Webster, R. G. ( 1999; ). Reassortment and insertion–deletion are strategies for the evolution of influenza B viruses in nature. Journal of Virology 73, 7343-7348.
    [Google Scholar]
  11. Nakagawa, N., Maeda, A., Kase, T., Kubota, R. & Okuno, Y. ( 1999; ). Rapid detection and identification of two lineages of influenza B strains with monoclonal antibodies. Journal of Virological Methods 79, 113-120.[CrossRef]
    [Google Scholar]
  12. Nakagawa, N., Kubota, R., Maeda, A., Nakagawa, T. & Okuno, Y. ( 2000; ). Heterogeneity of influenza B virus strains in one epidemic season differentiated by monoclonal antibodies and nucleotide sequences. Journal of Clinical Microbiology 38, 3467-3469.
    [Google Scholar]
  13. Nerome, R., Hiromoto, Y., Sugita, S., Tanabe, N., Ishida, M., Matsumoto, M., Lindstrom, S. E., Takahashi, T. & Nerome, K. ( 1998; ). Evolutionary characteristics of influenza B virus since its first isolation in 1940: dynamic circulation of deletion and insertion mechanism. Archives of Virology 143, 1569-1583.[CrossRef]
    [Google Scholar]
  14. Okuno, Y., Tanaka, K., Baba, K., Maeda, A., Kunita, N. & Ueda, S. ( 1990; ). Rapid focus reduction neutralization test of influenza A and B viruses in microtiter system. Journal of Clinical Microbiology 28, 1308-1313.
    [Google Scholar]
  15. Okuno, Y., Isegawa, Y., Sasao, F. & Ueda, S. ( 1993; ). A common neutralizing epitope conserved between the hemagglutinins of influenza A virus H1 and H2 strains. Journal of Virology 67, 2552-2558.
    [Google Scholar]
  16. Weis, W., Brown, J. H., Cusack, S., Paulson, J. C., Skehel, J. J. & Wiley, D. C. ( 1988; ). Structure of the influenza virus haemagglutinin completed with its receptor, sialic acid. Nature 333, 426-431.[CrossRef]
    [Google Scholar]
  17. Wilson, I. A., Skehel, J. J. & Wiley, D. C. ( 1981; ). Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 Å resolution. Nature 289, 366-373.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-9-2169
Loading
/content/journal/jgv/10.1099/0022-1317-82-9-2169
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error