1887

Abstract

Structural studies have implicated Cys, Cys and Cys of simian virus 40 (SV40) Vp1 in disulfide bond formation. Recently, we have shown the three cysteines to be essential for disulfide linkage of Vp1 complexes . Here, the role of the three cysteines was explored during the course of SV40 infection. Single-, double- and triple-mutant Vp1 at Cys, Cys and Cys continued to localize to the nuclei of transfected CV-1 cells and to bind DNA, but showed a range of abilities to form plaques. Only mutants containing the Cys→Ser change showed defects in plaque formation. Single mutants at Cys formed small plaques; mutants at Cys . Cys, Cys . Cys and Cys . Cys . Cys formed no plaques. All three isolated revertants contained back-mutations at the Vp1 Cys codon. These results further confirm the involvement of the three Vp1 cysteines in protein–protein interactions during virus assembly. Cys is critical for production of wild-type infectious virions, whereas Cys and Cys play secondary roles.

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2001-08-01
2020-01-25
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