1887

Abstract

Friend murine leukaemia virus (FrMLV) FrC6 clone A8 causes spongiform degeneration in the central nervous system (CNS) of newborn but not 3-week-old rats. To assess whether expression of the ecotropic MLV receptor (CAT-1) in the CNS correlates with the pathogenicity of the A8 virus, we generated an anti-CAT-1 antibody raised against a synthetic peptide that corresponds to the carboxyl-terminal amino acid sequence of CAT-1. In the CNS of newborn and 3 to 4-week-old rats, a strong immunoreactivity against the antibody was detected in most of the endothelial cells. However, almost no expression of CAT-1 was detected in the CNS of 21-week-old rats. In newborn rats, many parenchymal cells in the brain as well as the vascular wall expressed CAT-1 antigen. These findings suggest that retrovirus receptor-bearing glial cells contribute to the neuropathogenesis of MLV, including clone A8, which induces spongiosis in rats only when inoculated into newborns.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-8-1815
2001-08-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/8/0821815a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-8-1815&mimeType=html&fmt=ahah

References

  1. Albritton L. M., Tseng L., Scadden D., Cunningham J. M. 1989; A putative murine ecotropic retrovirus receptor gene encodes a multiple membrane-spanning protein and confers susceptibility to virus infection. Cell 57:659–666
    [Google Scholar]
  2. Albritton L. M., Kim J. W., Tseng L., Cunningham J. M. 1993; Envelope-binding domain in the cationic amino acid transporter determines the host range of ecotropic murine retroviruses. Journal of Virology 67:2091–2096
    [Google Scholar]
  3. Aulak K. S., Liu J., Wu J., Hyatt S. L., Puppi M., Henning S. J., Hatzoglou M. 1996; Molecular sites of regulation of expression of the rat cationic amino acid transporter gene. Journal of Biological Chemistry 271:29799–29806
    [Google Scholar]
  4. Baszler T. V., Zachary J. F. 1990; Murine retroviral-induced spongiform neuronal degeneration parallels resident microglial cell infection: ultrastructural findings. Laboratory Investigation 63:612–623
    [Google Scholar]
  5. Baszler T. V., Zachary J. F. 1991; Murine retroviral neurovirulence correlates with an enhanced ability of virus to infect selectively, replicate in, and activate resident microglial cells. American Journal of Pathology 138:655–671
    [Google Scholar]
  6. Braissant O., Gotoh T., Loup M., Mori M., Bachmann C. 1999; l-Arginine uptake, the citrulline-NO cycle and arginase II in the rat brain: an in situ hybridization study. Molecular Brain Research 70:231–241
    [Google Scholar]
  7. Clapp D. W., Dumenco L. L., Hatzoglou M., Gerson S. L. 1991; Fetal liver hematopoietic stem cells as a target for in utero retroviral gene transfer. Blood 78:1132–1139
    [Google Scholar]
  8. Czub M., Czub S., McAtee F. J., Portis J. L. 1991; Age-dependent resistance to murine retrovirus-induced spongiform neurodegeneration results from central nervous system-specific restriction of virus replication. Journal of Virology 65:2539–2544
    [Google Scholar]
  9. Czub M., Czub S., Rappold M., Mazgareanu S., Schwender S., Demuth M., Hein A., Dorries R. 1995; Murine leukemia virus-induced neurodegeneration of rats: enhancement of neuropathogenicity correlates with enhanced viral tropism for macrophages, microglia, and brain vascular cells. Virology 214:239–244
    [Google Scholar]
  10. Hatzoglou M., Lamers W., Bosch F., Wynshaw-Boris A., Clapp D. W., Hanson R. W. 1990; Hepatic gene transfer in animals using retroviruses containing the promoter from the gene for phosphoenolpyruvate carboxykinase. Journal of Biological Chemistry 265:17285–17293
    [Google Scholar]
  11. Ikeda T., Takase-Yoden S., Watanabe R. 1995; Characterization of monoclonal antibodies recognizing neurotropic Friend murine leukemia virus. Virus Research 38:297–304
    [Google Scholar]
  12. Ikeda H., Kato K., Suzuki T., Kitani H., Matsubara Y., Takase-Yoden S., Watanabe R., Kitagawa M., Aizawa S. 2000; Properties of the naturally occurring soluble surface glycoprotein of ecotropic murine leukemia virus: binding specificity and possible conformational change after binding to receptor. Journal of Virology 74:1815–1826
    [Google Scholar]
  13. Ito K., Groudine M. 1997; A new member of the cationic amino acid transporter family is preferentially expressed in adult mouse brain. Journal of Biological Chemistry 272:26780–26786
    [Google Scholar]
  14. Jaenisch R., Hoffmann E. 1979; Transcription of endogenous C-type viruses in resting and proliferating tissues of BALB/Mo mice. Virology 98:289–297
    [Google Scholar]
  15. Kai K., Furuta T. 1984; Isolation of paralysis-inducing murine leukemia viruses from Friend virus passaged in rats. Journal of Virology 50:970–973
    [Google Scholar]
  16. Kai K., Mitsuno K., Goto N., Ami Y., Ando S., Kanoe M. 1996; Factors affecting induction of neurological disorders in mice by paralysis-inducing Friend-related PVC viruses. Journal of Veterinary Medical Science 58:285–290
    [Google Scholar]
  17. Kavanaugh M. P., Wang H., Boyd C. A., North R. A., Kabat D. 1994; Cell surface receptor for ecotropic host-range mouse retroviruses: a cationic amino acid transporter. Archives of Virology Supplementum 9:485–494
    [Google Scholar]
  18. Kim J. W., Cunningham J. M. 1993; N-linked glycosylation of the receptor for murine ecotropic retroviruses is altered in virus-infected cells. Journal of Biological Chemistry 268:16316–16320
    [Google Scholar]
  19. Kim J. W., Closs E. I., Albritton L. M., Cunningham J. M. 1991; Transport of cationic amino acids by the mouse ecotropic retrovirus receptor. Nature 352:725–728
    [Google Scholar]
  20. Lynch W. P., Czub S., McAtee F. J., Hayes S. F., Portis J. L. 1991; Murine retrovirus-induced spongiform encephalopathy: productive infection of microglia and cerebellar neurons in accelerated CNS disease. Neuron 7:365–379
    [Google Scholar]
  21. Lynch W. P., Robertson S. J., Portis J. L. 1995; Induction of focal spongiform neurodegeneration in developmentally resistant mice by implantation of murine retrovirus-infected microglia. Journal of Virology 69:1408–1419
    [Google Scholar]
  22. MacLeod C. L. 1996; Regulation of cationic amino acid transporter (CAT) gene expression. Biochemical Society Transactions 24:846–852
    [Google Scholar]
  23. Masuda M., Remington M. P., Hoffman P. M., Ruscetti S. K. 1992; Molecular characterization of a neuropathogenic and nonerythroleukemogenic variant of Friend murine leukemia virus PVC-211. Journal of Virology 66:2798–2806
    [Google Scholar]
  24. Masuda M., Kakushima N., Wilt S. G., Ruscetti S. K., Hoffman P. M., Iwamoto A., Masuda M. 1999; Analysis of receptor usage by ecotropic murine retroviruses, using green fluorescent protein-tagged cationic amino acid transporters. Journal of Virology 73:8623–8629
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  26. Stoll J., Wadhwani K. C., Smith Q. R. 1993; Identification of the cationic amino acid transporter (System y+) of the rat blood–brain barrier. Journal of Neurochemistry 60:1956–1959
    [Google Scholar]
  27. Takase-Yoden S., Watanabe R. 1997; Unique sequence and lesional tropism of a new variant of neuropathogenic Friend murine leukemia virus. Virology 233:411–422
    [Google Scholar]
  28. Takase-Yoden S., Watanabe R. 1999; Contribution of virus–receptor interaction to distinct viral proliferation of neuropathogenic and nonneuropathogenic murine leukemia viruses in rat glial cells. Journal of Virology 73:4461–4464
    [Google Scholar]
  29. Wang H., Kavanaugh M. P., North R. A., Kabat D. 1991; Cell-surface receptor for ecotropic murine retroviruses is a basic amino-acid transporter. Nature 352:729–731
    [Google Scholar]
  30. Watanabe R., Takase-Yoden S. 1995; Gene expression of neurotropic retrovirus in the CNS. Progress in Brain Research 105:255–262
    [Google Scholar]
  31. White M. F., Christensen H. N. 1982; Cationic amino acid transport into cultured animal cells. II. Transport system barely perceptible in ordinary hepatocytes, but active in hepatoma cell lines. Journal of Biological Chemistry 257:4450–4457
    [Google Scholar]
  32. Wu J. Y., Robinson D., Kung H. J., Hatzoglou M. 1994; Hormonal regulation of the gene for the type C ecotropic retrovirus receptor in rat liver cells. Journal of Virology 68:1615–1623
    [Google Scholar]
  33. Yoshimoto T., Yoshimoto E., Meruelo D. 1992; Enhanced gene expression of the murine ecotropic retroviral receptor and its human homolog in proliferating cells. Journal of Virology 66:4377–4381
    [Google Scholar]
  34. Yoshimoto T., Yoshimoto E., Meruelo D. 1993; Identification of amino acid residues critical for infection with ecotropic murine leukemia retrovirus. Journal of Virology 67:1310–1314
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-8-1815
Loading
/content/journal/jgv/10.1099/0022-1317-82-8-1815
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error