1887

Abstract

Genetic diversity was characterized in 14 isolates of (GFLV) recovered from grapevine (). Virions were collected by immunocapture, and a 1557 bp fragment containing part of the viral coat protein gene and part of the untranslated region to its 3′ side was amplified by RT–PCR. Sequence variation among isolates was characterized by restriction fragment length polymorphism (RFLP) analysis and by sequencing. The II-generated RFLP patterns from the various isolates were highly variable. The isolates were passaged in . The RFLP patterns altered with passage through the alternate host, but the variation stabilized after a number of passages. Individual genomes were recovered by cloning. The subcloned sequences were found to vary from each other by as much as 13%, and the encoded amino acid sequences by as much as 9%. The data suggest that the GFLV genome consists of quasispecies populations.

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2001-07-01
2019-10-22
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