1887

Abstract

Infectious salmon anaemia virus (ISAV) is an orthomyxo-like virus that causes serious disease in Atlantic salmon (). Like the orthomyxoviruses, ISAV has been shown to possess haemagglutinin (HA) activity. This study presents the cloning, expression and identification of the ISAV HA gene, which was isolated from a cDNA library by immunoscreening. The HA gene contained an ISAV-specific conserved nucleotide motif in the 5′ region and a 1167 bp open reading frame encoding a protein with a predicted molecular mass of 42·4 kDa. The HA gene was expressed in a baculovirus system. A monoclonal antibody (MAb) shown previously to be directed against the ISAV HA reacted with insect cells infected with recombinant baculovirus. Salmon erythrocytes also adsorbed to these cells and adsorption was inhibited by the addition of either the ISAV-specific MAb or a polyclonal rabbit serum prepared against purified virus, confirming the virus specificity of the reaction. Immunoblot analyses indicated that ISAV HA, in contrast to influenza virus HA, is not posttranslationally cleaved. Sequence comparisons of the HA gene from five Norwegian, one Scottish and one Canadian isolate revealed a highly polymorphic region that may be useful in epidemiological studies.

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2001-07-01
2019-09-18
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References

  1. Blake, S., Bouchard, D., Keleher, W., Opitz, M. & Nicholson, B. L. ( 1999; ). Genomic relationships of the North American isolate of infectious salmon anaemia virus (ISAV) to the Norwegian strain of ISAV. Diseases of Aquatic Organisms 35, 139-144.[CrossRef]
    [Google Scholar]
  2. Cunningham, C. O. & Snow, M. ( 2000; ). Genetic analysis of infectious salmon anaemia virus (ISAV) from Scotland. Diseases of Aquatic Organisms 41, 1-8.[CrossRef]
    [Google Scholar]
  3. Devold, M., Krossøy, B., Aspehaug, V. & Nylund, A. ( 2000; ). Use of RT–PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. Diseases of Aquatic Organisms 40, 9-18.[CrossRef]
    [Google Scholar]
  4. Eliassen, T. M., Frøystad, M. K., Dannevig, B. H., Jankowska, M., Brech, A., Falk, K., Romøren, K. & Gjøen, T. ( 2000; ). Initial events in infectious salmon anaemia virus infection: evidence for the requirement of a low-pH step. Journal of Virology 74, 218-227.[CrossRef]
    [Google Scholar]
  5. Falk, K., Namork, E., Rimstad, E., Mjaaland, S. & Dannevig, B. H. ( 1997; ). Characterization of infectious salmon anaemia virus, an orthomyxo-like virus isolated from Atlantic salmon (Salmo salar L.). Journal of Virology 71, 9016-9023.
    [Google Scholar]
  6. Falk, K., Namork, E. & Dannevig, B. H. ( 1998; ). Characterization and applications of a monoclonal antibody against infectious salmon anaemia virus. Diseases of Aquatic Organisms 34, 77-85.[CrossRef]
    [Google Scholar]
  7. Feldmann, H., Kretzschmar, E., Klingeborn, B., Rott, R., Klenk, H. D. & Garten, W. ( 1988; ). The structure of serotype H10 haemagglutinin of influenza A virus: comparison of an apathogenic avian and a mammalian strain pathogenic for mink. Virology 165, 428-437.[CrossRef]
    [Google Scholar]
  8. Herrler, G., Compans, R. W. & Meier-Ewert, H. ( 1979; ). A precursor glycoprotein in influenza C virus. Virology 99, 49-56.[CrossRef]
    [Google Scholar]
  9. Huang, R. T., Rott, R. & Klenk, H. D. ( 1981; ). Influenza viruses cause hemolysis and fusion of cells. Virology 110, 243-247.[CrossRef]
    [Google Scholar]
  10. Kibenge, F. S. B., Lyaku, J. R., Rainnie, D. & Hammell, K. L. ( 2000; ). Growth of infectious salmon anaemia virus in CHSE-214 cells and evidence for phenotypic differences between virus strains. Journal of General Virology 81, 143-150.
    [Google Scholar]
  11. Kitame, F., Sugawara, K., Ohwada, K. & Homma, M. ( 1982; ). Proteolytic activation of hemolysis and fusion by influenza C virus. Archives of Virology 73, 357-361.[CrossRef]
    [Google Scholar]
  12. Koren, C. W. R. & Nylund, A. ( 1997; ). Morphology and morphogenesis of infectious salmon anaemia virus replicating in the endothelium of Atlantic salmon Salmo salar. Diseases of Aquatic Organisms 29, 99-109.[CrossRef]
    [Google Scholar]
  13. Krossøy, B., Hordvik, I., Nilsen, F., Nylund, A. & Endresen, C. ( 1999; ). The putative polymerase sequence of infectious salmon anaemia virus suggests a new genus within the Orthomyxoviridae. Journal of Virology 73, 2136-2142.
    [Google Scholar]
  14. Krossøy, B., Nilsen, F., Falk, K., Endresen, C. & Nylund, A. ( 2001; ). Phylogenetic analysis of infectious salmon anaemia virus isolates from Norway, Canada and Scotland. Diseases of Aquatic Organisms 44, 1-6.[CrossRef]
    [Google Scholar]
  15. Lazarowitz, S. G., Compans, R. W. & Choppin, P. W. ( 1973; ). Proteolytic cleavage of the haemagglutinin polypeptide of influenza virus. Function of the uncleaved polypeptide HA. Virology 52, 199-212.[CrossRef]
    [Google Scholar]
  16. Maeda, T. & Ohnishi, S. ( 1980; ). Activation of influenza virus by acidic media causes hemolysis and fusion of erythrocytes. FEBS Letters 122, 283-287.[CrossRef]
    [Google Scholar]
  17. Maeda, T., Kawasaki, K. & Ohnishi, S. ( 1981; ). Interaction of influenza virus haemagglutinin with target membrane lipids is a key step in virus-induced hemolysis and fusion at pH 5·2. Proceedings of the National Academy of Sciences, USA 78, 4133-4137.[CrossRef]
    [Google Scholar]
  18. Mjaaland, S., Rimstad, E., Falk, K. & Dannevig, B. H. ( 1997; ). Genomic characterization of the virus causing infectious salmon anaemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost. Journal of Virology 71, 7681-7686.
    [Google Scholar]
  19. Mullins, J. E., Groman, D. & Wadowska, D. ( 1998; ). Infectious salmon anaemia in salt water Atlantic salmon (Salmo salar L.) in New Brunswick, Canada. Bulletin of the European Association of Fish Pathologists 18, 110-114.
    [Google Scholar]
  20. Ohuchi, M., Ohuchi, R. & Mifune, K. ( 1982; ). Demonstration of hemolytic and fusion activities of influenza C virus. Journal of Virology 42, 1076-1079.
    [Google Scholar]
  21. Rodger, H. D., Turnbull, T., Muir, F., Millar, S. & Richards, R. H. ( 1998; ). Infectious salmon anaemia (ISA) in the United Kingdom. Bulletin of the European Association of Fish Pathologists 18, 115-116.
    [Google Scholar]
  22. Rosenthal, P. B., Zhang, X., Formanowski, F., Fitz, W., Wong, C. H., Meier-Ewert, H., Skehel, J. J. & Wiley, D. C. ( 1998; ). Structure of the haemagglutinin–esterase–fusion glycoprotein of influenza C virus. Nature 396, 92-96.[CrossRef]
    [Google Scholar]
  23. Sandvik, T., Rimstad, E. & Mjaaland, S. ( 2000; ). The viral RNA 3′- and 5′-end structure and mRNA transcription of infectious salmon anaemia virus resemble those of influenza viruses. Archives of Virology 145, 1659-1669.[CrossRef]
    [Google Scholar]
  24. Skehel, J. J. & Wiley, D. C. ( 2000; ). Receptor binding and membrane fusion in virus entry: the influenza haemagglutinin. Annual Review of Biochemistry 69, 531-569.[CrossRef]
    [Google Scholar]
  25. Szilvay, A. M., Nornes, S., Haugan, I. R., Olsen, L., Prasad, V. R., Endresen, C., Goff, S. P. & Helland, D. E. ( 1992; ). Epitope mapping of HIV reverse transcriptase with monoclonal antibodies that inhibit the polymerase and RNase H activity. Journal of Acquired Immune Deficiency Syndromes 5, 647-657.
    [Google Scholar]
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673-4680.[CrossRef]
    [Google Scholar]
  27. Thorud, K. & Djupvik, H. O. ( 1988; ). Infectious salmon anaemia in Atlantic salmon (Salmo salar L.). Bulletin of the European Association of Fish Pathologists 8, 109-111.
    [Google Scholar]
  28. Wagner, R., Geyer, H., Geyer, R. & Klenk, H.-D. ( 1996; ). N-acetyl-β-glucosaminidase accounts for differences in glycosylation of influenza virus hemagglutinin expressed in insect cells from a baculovirus vector. Journal of Virology 70, 4103-4109.
    [Google Scholar]
  29. Webster, R. G., Bean, W. J., Gorman, O. T., Chambers, T. M. & Kawaoka, Y. ( 1992; ). Evolution and ecology of influenza A viruses. Microbiological Reviews 56, 152-179.
    [Google Scholar]
  30. White, J., Matlin, K. & Helenius, A. ( 1981; ). Cell fusion by Semliki Forest, influenza, and vesicular stomatitis viruses. Journal of Cell Biology 89, 674-679.[CrossRef]
    [Google Scholar]
  31. Wiley, D. C. & Skehel, J. J. ( 1987; ). The structure and function of the haemagglutinin membrane glycoprotein of influenza virus. Annual Review of Biochemistry 56, 365-394.[CrossRef]
    [Google Scholar]
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