1887

Abstract

A previous report showed that insertion of a foreign gene encoding chloramphenicol acetyltransferase (CAT) between the HN and L genes of the full-length cDNA of a virulent Newcastle disease virus (NDV) yielded virus with growth retardation and attenuation. The NDV vector used in that study was pathogenic to chickens; it is therefore not suitable for use as a vaccine vector. In the present study, an avirulent NDV vector was generated and its potential to express CAT protein was evaluated. The CAT gene was under the control of NDV transcriptional start and stop signals and was inserted immediately before the open reading frame of the viral 3′-proximal nucleocapsid protein gene. A recombinant NDV expressing CAT activity at a high level was recovered. The replication and pathogenesis of the CAT-expressing recombinant NDV were not modified significantly. These results indicate the potential utility of an avirulent NDV as a vaccine vector.

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2001-07-01
2019-09-20
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References

  1. Alexander, D. J. ( 1997; ). Newcastle disease and other avian Paramyxoviridae infections. In Diseases of Poultry , pp. 541-569. Edited by B. W. Calnek. Ames, IA:Iowa State University Press.
  2. Bukreyev, A., Camargo, E. & Collins, P. L. ( 1996; ). Recovery of infectious respiratory syncytial virus expressing an additional, foreign gene. Journal of Virology 70, 6634-6641.
    [Google Scholar]
  3. Byrappa, S., Gavin, D. K. & Gupta, K. C. ( 1995; ). A highly efficient procedure for site-specific mutagenesis of full-length plasmids using Vent DNA polymerase. Genome Research 5, 404-407.[CrossRef]
    [Google Scholar]
  4. Calain, P. & Roux, L. ( 1993; ). The rule of six, a basic feature for efficient replication of Sendai virus defective interfering RNA. Journal of Virology 67, 4822-4830.
    [Google Scholar]
  5. Conzelmann, K.-K. ( 1996; ). Genetic manipulation of non-segmented negative-strand RNA viruses. Journal of General Virology 77, 381-389.[CrossRef]
    [Google Scholar]
  6. de Leeuw, O. & Peeters, B. ( 1999; ). Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae. Journal of General Virology 80, 131-136.
    [Google Scholar]
  7. Haglund, K., Forman, J., Kräusslich, H. G. & Rose, J. K. ( 2000; ). Expression of human immunodeficiency virus type 1 Gag protein precursor and envelope proteins from a vesicular stomatitis virus recombinant: high-level production of virus-like particles containing HIV envelope. Virology 268, 112-121.[CrossRef]
    [Google Scholar]
  8. Hasan, M. K., Kato, A., Shioda, T., Sakai, Y., Yu, D. & Nagai, Y. ( 1997; ). Creation of an infectious recombinant Sendai virus expressing the firefly luciferase gene from the 3′ proximal first locus. Journal of General Virology 78, 2813-2820.
    [Google Scholar]
  9. He, B., Paterson, R. G., Ward, C. D. & Lamb, R. A. ( 1997; ). Recovery of infectious SV5 from cloned DNA and expression of a foreign gene. Virology 237, 249-260.[CrossRef]
    [Google Scholar]
  10. Kingsbury, D. W. ( 1966; ). Newcastle disease virus. I. Isolation and preliminary characterization of RNA from virus particles. Journal of Molecular Biology 18, 195-203.[CrossRef]
    [Google Scholar]
  11. Krishnamurthy, S. & Samal, S. K. ( 1998; ). Nucleotide sequences of the trailer, nucleocapsid protein gene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of the entire genome sequence. Journal of General Virology 79, 2419-2424.
    [Google Scholar]
  12. Krishnamurthy, S., Huang, Z. & Samal, S. K. ( 2000; ). Recovery of a virulent strain of Newcastle disease virus from cloned cDNA: expression of a foreign gene results in growth retardation and attenuation. Virology 278, 168-182.[CrossRef]
    [Google Scholar]
  13. Lamb, R. A. & Kolakofsky, D. ( 1996; ). Paramyxoviridae: the viruses and their replication. In Fields Virology , pp. 1177-1204. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia:Lippincott–Raven.
  14. Mebatsion, T., Schnell, M. J., Cox, J. H., Finke, S. & Conzelmann, K. K. ( 1996; ). Highly stable expression of a foreign gene from rabies virus vectors. Proceedings of the National Academy of Sciences, USA 93, 7310-7314.[CrossRef]
    [Google Scholar]
  15. Nagai, Y. ( 1999; ). Paramyxovirus replication and pathogenesis. Reverse genetics transforms understanding. Reviews in Medical Virology 9, 83-99.[CrossRef]
    [Google Scholar]
  16. Palese, P., Zheng, H., Engelhardt, O. G., Pleschka, S. & Garcia-Sastre, A. ( 1996; ). Negative-strand RNA viruses: genetic engineering and applications. Proceedings of the National Academy of Sciences, USA 93, 11354-11358.[CrossRef]
    [Google Scholar]
  17. Peeples, M. E. ( 1988; ). Newcastle disease virus replication. In Newcastle Disease , pp. 45-78. Edited by D. J. Alexander. Dordrecht:Kluwer Academic.
  18. Peeters, B. P., de Leeuw, O. S., Koch, G. & Gielkens, A. L. ( 1999; ). Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. Journal of Virology 73, 5001-5009.
    [Google Scholar]
  19. Peeters, B. P., Gruijthuijsen, Y. K., de Leeuw, O. S. & Gielkens, A. L. ( 2000; ). Genome replication of Newcastle disease virus: involvement of the rule-of-six. Archives of Virology 145, 1829-1845.[CrossRef]
    [Google Scholar]
  20. Phillips, R. J., Samson, A. R. & Emmerson, P. T. ( 1998; ). Nucleotide sequence of the 5′-terminus of Newcastle disease virus and assembly of the complete genomic sequence: agreement with the ‘rule of six’. Archives of Virology 143, 1993-2002.[CrossRef]
    [Google Scholar]
  21. Pringle, C. R. ( 1997; ). The order Mononegavirales – current status. Archives of Virology 142, 2321-2326.
    [Google Scholar]
  22. Rima, B., Alexander, D. J., Billeter, M. A., Collins, P. L., Kingsbury, D. W., Lipkind, M. A., Nagai, Y., Örvell, C., Pringle, C. R. & ter Meulen, V. ( 1995; ). Family Paramyxoviridae. In Virus Taxonomy. Sixth Report of the International Committee on the Taxonomy of Viruses , pp. 268-274. Edited by F. A. Murphy, C. M. Fauquet, D. H. Bishop, S. A. Ghabrial, A. W. Jarvis, G. P. Martelli, M. A. Mayo & M. D. Summers. Vienna & New York:Springer-Verlag.
  23. Römer-Oberdörfer, A., Mundt, E., Mebatsion, T., Buchholz, U. J. & Mettenleiter, T. C. ( 1999; ). Generation of recombinant lentogenic Newcastle disease virus from cDNA. Journal of General Virology 80, 2987-2995.
    [Google Scholar]
  24. Sakai, Y., Kiyotani, K., Fukumura, M., Asakawa, M., Kato, A., Shioda, T., Yoshida, T., Tanaka, A., Hasegawa, M. & Nagai, Y. ( 1999; ). Accommodation of foreign genes into the Sendai virus genome: sizes of inserted genes and viral replication. FEBS Letters 456, 221-226.[CrossRef]
    [Google Scholar]
  25. Schnell, M. J., Buonocore, L., Whitt, M. A. & Rose, J. K. ( 1996; ). The minimal conserved transcription stop–start signal promotes stable expression of a foreign gene in vesicular stomatitis virus. Journal of Virology 70, 2318-2323.
    [Google Scholar]
  26. Steward, M., Vipond, I. B., Millar, N. S. & Emmerson, P. T. ( 1993; ). RNA editing in Newcastle disease virus. Journal of General Virology 74, 2539-2547.[CrossRef]
    [Google Scholar]
  27. Wertz, G. W., Perepelitsa, V. P. & Ball, L. A. ( 1998; ). Gene rearrangement attenuates expression and lethality of a nonsegmented negative strand RNA virus. Proceedings of the National Academy of Sciences, USA 95, 3501-3506.[CrossRef]
    [Google Scholar]
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