1887

Abstract

This work focuses on the development of a potential recombinant DNA vaccine against foot-and-mouth disease virus (FMDV). Such a vaccine would have significant advantages over the conventional inactivated virus vaccine, in particular having none of the risks associated with the high security requirements for working with live virus. The principal aim of this strategy was to stimulate an antibody response to native, neutralizing epitopes of empty FMDV capsids generated . Thus, a plasmid (pcDNA3.1/P1–2A3C3D) was constructed containing FMDV cDNA sequences encoding the viral structural protein precursor P1–2A and the non-structural proteins 3C and 3D. The 3C protein was included to ensure cleavage of the P1–2A precursor to VP0, VP1 and VP3, the components of self-assembling empty capsids. The non-structural protein 3D was also included in the construct in order to provide additional stimulation of CD4 T cells. When swine were immunized with this plasmid, antibodies to FMDV and the 3D polymerase were synthesized. Furthermore, neutralizing antibodies were detected and, after three sequential vaccinations with DNA, some of the animals were protected against challenge with live virus. Additional experiments suggested that the antibody response to FMDV proteins was improved by the co-administration of a plasmid encoding porcine granulocyte–macrophage colony-stimulating factor. Although still not as effective as the conventional virus vaccine, the results encourage further work towards the development of a DNA vaccine against FMDV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-7-1713
2001-07-01
2019-09-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/7/0821713a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-7-1713&mimeType=html&fmt=ahah

References

  1. Abrams, C. C., King, A. M. Q. & Belsham, G. J. ( 1995; ). Assembly of foot-and-mouth disease virus empty capsids synthesized by a vaccinia virus expression system. Journal of General Virology 76, 3089-3098.[CrossRef]
    [Google Scholar]
  2. Bautista, E. M. & Molitor, T. W. ( 1997; ). Cell mediated immunity to porcine reproductive and respiratory syndrome virus in swine. Viral Immunology 10, 83-94.[CrossRef]
    [Google Scholar]
  3. Belsham, G. J. ( 1993; ). Distinctive features of foot-and-mouth disease virus, a member of the picornavirus family; aspects of virus protein synthesis, protein processing and structure. Progress in Biophysics and Molecular Biology 60, 241-260.[CrossRef]
    [Google Scholar]
  4. Belsham, G. J., Brangwyn, J. K., Ryan, M. D., Abrams, C. C. & King, A. M. Q. ( 1990; ). Intracellular expression and processing of foot-and-mouth disease virus capsid precursors using vaccinia virus vectors: influence of the L protease. Virology 176, 524-530.[CrossRef]
    [Google Scholar]
  5. Chinsangaram, J., Beard, C., Mason, P. W., Zellner, M. K., Ward, G. & Grubman, M. J. ( 1998; ). Antibody response in mice inoculated with DNA expressing foot-and-mouth disease virus capsid proteins. Journal of Virology 72, 4454-4457.
    [Google Scholar]
  6. Collen, T. ( 1994; ). Foot and mouth disease (aphthovirus): viral T cell epitopes. In Cell-Mediated Immunity in Ruminants , pp. 173-197. Edited by B. M. L. Goddeeris & W. I. Morrison. Boca Raton, FL:CRC Press.
  7. Collen, T., Pullen, L. & Doel, T. R. ( 1989; ). T cell-dependent induction of antibody against foot-and-mouth disease virus in a mouse model. Journal of General Virology 70, 395-403.[CrossRef]
    [Google Scholar]
  8. Collen, T., Baron, J., Childerstone, A., Corteyn, A., Doel, T. R., Flint, M., Garcia-Valcarcel, M., Parkhouse, R. M. E. & Ryan, M. D. ( 1998; ). Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: the polymerase (P3Dpol) as an immunodominant T-cell immunogen. Virus Research 56, 125-133.[CrossRef]
    [Google Scholar]
  9. DiMarchi, R., Brooke, G., Gale, C., Cracknell, V., Doel, T. R. & Mowat, N. ( 1986; ). Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232, 639-641.[CrossRef]
    [Google Scholar]
  10. Ellard, F. M., Drew, J., Blakemore, W. E., Stuart, D. I. & King, A. M. Q. ( 1999; ). Evidence for the role of His-142 of protein 1C in the acid-induced disassembly of foot-and-mouth disease virus capsids. Journal of General Virology 80, 1911-1918.
    [Google Scholar]
  11. Foster, M., Cook, A., Cedillo, L. & Parkhouse, R. M. E. ( 1998; ). Serological and cellular immune responses to non-structural proteins in animals infected with FMDV. Veterinary Quarterly 20, S28-S30.
    [Google Scholar]
  12. Foster-Cuevas, M. (1996). Immunodeterminants of foot-and-mouth disease virus. PhD thesis, University of Hertfordshire, UK.
  13. Francis, M. J., Fry, C. M., Rowlands, D. J., Brown, F., Bittle, J. L., Houghten, R. A. & Lerner, R. A. ( 1985; ). Immunological priming with synthetic peptides of foot-and-mouth disease virus. Journal of General Virology 66, 2347-2354.[CrossRef]
    [Google Scholar]
  14. Francis, M. J., Hastings, G. Z., Syred, A. D., McGinn, B., Brown, F. & Rowlands, D. J. ( 1987; ). Non-responsiveness to a foot-and-mouth disease virus peptide overcome by addition of foreign helper T-cell determinants. Nature 330, 168-170.[CrossRef]
    [Google Scholar]
  15. Fuerst, T. R., Niles, E. G., Studier, F. W. & Moss, B. ( 1986; ). Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proceedings of the National Academy of Sciences, USA 83, 8122-8126.[CrossRef]
    [Google Scholar]
  16. Garcia-Valcarcel, M., Doel, T., Collen, T., Ryan, M. & Parkhouse, R. M. E. ( 1996; ). Recognition of foot-and-mouth disease virus and its capsid protein VP1 by bovine peripheral T lymphocytes. Journal of General Virology 77, 727-735.[CrossRef]
    [Google Scholar]
  17. Geysen, H. M., Meloen, R. H. & Barteling, S. J. ( 1984; ). Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proceedings of the National Academy of Sciences, USA 81, 3998-4002.[CrossRef]
    [Google Scholar]
  18. Golding, S. M., Hedger, R. S. & Talbot, P. ( 1976; ). Radial immuno-diffusion and serum-neutralisation techniques for the assay of antibodies to swine vesicular disease. Research in Veterinary Science 20, 142-147.
    [Google Scholar]
  19. Grubman, M. J., Morgan, D. O., Kendall, J. & Baxt, B. ( 1985; ). Capsid intermediates assembled in a foot-and-mouth disease virus genome RNA-programmed cell-free translation system and in infected cells. Journal of Virology 56, 120-126.
    [Google Scholar]
  20. Katrak, K., Mahon, B. P., Jones, W. C., Brautigam, S. & Mills, K. H. ( 1992; ). Preparative separation of foreign antigens for highly efficient presentation of T cells in vitro. Journal of Immunological Methods 156, 247-254.[CrossRef]
    [Google Scholar]
  21. Kim, J. J., Bagarazzi, M. L., Trivedi, N., Hu, Y., Kazahaya, K., Wilson, D. M., Ciccarelli, R., Chattergoon, M. A., Dang, K., Mahalingam, S., Chalian, A. A., Agadjanyan, M. G., Boyer, J. D., Wang, B. & Weiner, D. B. ( 1997; ). Engineering of in vivo immune responses to DNA immunization via codelivery of costimulatory molecule genes. Nature Biotechnology 15, 641-646.[CrossRef]
    [Google Scholar]
  22. Lewis, S. A., Morgan, D. O. & Grubman, M. J. ( 1991; ). Expression, processing, and assembly of foot-and-mouth disease virus capsid structures in heterologous systems: induction of a neutralizing antibody response in guinea pigs. Journal of Virology 65, 6572-6580.
    [Google Scholar]
  23. McCullough, K. C., De Simone, F., Brocchi, E., Capucci, L., Crowther, R. J. & Kihm, U. ( 1992; ). Protective immune response against foot-and-mouth disease. Journal of Virology 66, 1835-1840.
    [Google Scholar]
  24. Mayr, G. A., Chinsangaram, J. & Grubman, M. J. ( 1999; ). Development of replication-defective adenovirus serotype 5 containing the capsid and 3C protease coding regions of foot-and-mouth disease virus as a vaccine candidate. Virology 263, 496-506.[CrossRef]
    [Google Scholar]
  25. Rodriguez, A., Saiz, J. C., Novella, I. S., Andreu, D. & Sobrino, F. ( 1994; ). Antigenic specificity of porcine T cell response against foot-and-mouth disease virus structural proteins: identification of T helper epitopes in VP1. Virology 205, 24-33.[CrossRef]
    [Google Scholar]
  26. Roosien, J., Belsham, G. J., Ryan, M. D., King, A. M. Q. & Vlak, J. M. ( 1990; ). Synthesis of foot-and-mouth disease virus capsid proteins in insect cells using baculovirus expression vectors. Journal of General Virology 71, 1703-1711.[CrossRef]
    [Google Scholar]
  27. Rowlands, D. J., Sangar, D. V. & Brown, F. ( 1975; ). A comparative chemical and serological study of the full and empty particles of foot-and-mouth disease virus. Journal of General Virology 26, 227-238.[CrossRef]
    [Google Scholar]
  28. Rweyemamu, M. M., Terry, G. & Pay, T. W. F. ( 1979; ). Stability and immunogenicity of empty particles of foot-and-mouth disease virus. Archives of Virology 59, 69-79.[CrossRef]
    [Google Scholar]
  29. Ryan, M. D., Belsham, G. J. & King, A. M. Q. ( 1989; ). Specificity of enzyme–substrate interactions in foot-and-mouth disease virus polyprotein processing. Virology 173, 35-45.[CrossRef]
    [Google Scholar]
  30. Saiz, J. C., Cairo, J., Medina, M., Zuidema, D., Abrams, C., Belsham, G. J., Domingo, E. & Vlak, J. M. ( 1994; ). Unprocessed foot-and-mouth disease virus capsid precursor displays discontinuous epitopes involved in viral neutralization. Journal of Virology 68, 4557-4564.
    [Google Scholar]
  31. Salt, J. S., Barnett, P. V., Dani, P. & Williams, L. ( 1998; ). Emergency vaccination of pigs against foot-and-mouth disease: protection against disease and reduction in contact transmission. Vaccine 16, 746-754.[CrossRef]
    [Google Scholar]
  32. Sanz-Parra, A., Vázquez, B., Sobrino, F., Cox, S. J., Ley, V. & Salt, J. S. ( 1999; ). Evidence of partial protection against foot-and-mouth disease in cattle immunized with a recombinant adenovirus vector expressing the precursor polypeptide (P1) of foot-and-mouth disease virus capsid proteins. Journal of General Virology 80, 671-679.
    [Google Scholar]
  33. Somasundaram, C., Takamatsu, H., Andreoni, C., Audonnet, J. C., Fischer, L., Lefèvre, F. & Charley, B. ( 1999; ). Enhanced protective response and immuno-adjuvant effects of porcine GM-CSF on DNA vaccination of pigs against Aujeszky’s disease virus. Veterinary Immunology and Immunopathology 70, 277-287.[CrossRef]
    [Google Scholar]
  34. Tuteja, R., Li, T. C., Takeda, N. & Jameel, S. ( 2000; ). Augmentation of immune responses to hepatitis E virus ORF2 DNA vaccination by codelivery of cytokine genes. Viral Immunology 13, 169-178.[CrossRef]
    [Google Scholar]
  35. van Bekkum, J. G. (1969). Correlation between serum antibody level and protection against challenge with FMD virus. In Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease, Brescia, Italy, pp. 38–40. Rome: FAO.
  36. Ward, G., Rieder, E. & Mason, P. W. ( 1997; ). Plasmid DNA encoding replicating foot-and-mouth disease virus genomes induces antiviral immune responses in swine. Journal of Virology 71, 7442-7447.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-7-1713
Loading
/content/journal/jgv/10.1099/0022-1317-82-7-1713
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error