@article{mbs:/content/journal/jgv/10.1099/0022-1317-82-7-1601, author = "Walther-Jallow, Lilian and Nilsson, Charlotta and Söderlund, Johan and ten Haaft, Peter and Mäkitalo, Barbro and Biberfeld, Peter and Böttiger, Per and Heeney, Jonathan and Biberfeld, Gunnel and Thorstensson, Rigmor", title = "Cross-protection against mucosal simian immunodeficiency virus (SIVsm) challenge in human immunodeficiency virus type 2-vaccinated cynomolgus monkeys", journal= "Journal of General Virology", year = "2001", volume = "82", number = "7", pages = "1601-1612", doi = "https://doi.org/10.1099/0022-1317-82-7-1601", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-82-7-1601", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "In this study we compared the efficacy of live attenuated human immunodeficiency virus type 2 (HIV-2) vaccine alone versus boosting with live non-pathogenic HIV-2 following priming with ALVAC HIV-2 (recombinant canarypox virus expressing HIV-2 env, gag and pol). Six monkeys were first inoculated intravenously with live HIV-2SBL-6669 and 7 to 10 months later were challenged intrarectally with 10 MID50 of cell-free simian immunodeficiency virus (SIV) strain SIVsm. One monkey was completely protected against SIV infection and all five monkeys that became SIV-infected showed a lower virus replication and an initial lower virus load as compared with a parallel group of six control animals. In another experiment five monkeys were immunized either three times with ALVAC HIV-2 alone or twice with ALVAC HIV-2 and once with purified native HIV-2 gp125. The monkeys were then challenged with HIV-2 given intravenously and finally with pathogenic SIVsm given intrarectally. After challenge with SIVsm, three of five monkeys were completely protected against SIVsm infection whereas the remaining two macaques became SIV-infected but with limited virus replication. In conclusion, vaccination with an ALVAC HIV-2 vaccine followed by exposure to live HIV-2 could induce cross-protection against mucosal infection with SIVsm and seemed to be more efficient than immunization with a live HIV-2 vaccine only.", }