1887

Abstract

Feline leukaemia virus (FeLV) infection in domestic cats can vary in its outcome (persistent, transient, no infection) for reasons that are not entirely known. It was hypothesized that the initial virus and provirus load could significantly influence the course of retrovirus infection. To determine the role of provirus loads, two methods of PCR, a nested PCR and a fluorogenic probe-based (TaqMan) real-time quantitative PCR, which were specific to the U3 region of FeLV-A were established. FeLV provirus in naturally and experimentally infected cats was then measured. Only 3 weeks after experimental FeLV-A infection, persistently infected cats demonstrated higher provirus loads and lower humoral immune responses than cats that had overcome antigenaemia. Lower initial provirus loads were associated with successful humoral immune responses. Unexpectedly, provirus in the buffy-coat cells of two cats that tested negative for the p27 antigen (a marker for viraemia) was also detected. In 597 Swiss cats, comparison of p27 antigen levels with PCR results revealed broad agreement. However, similar to the experimental situation, a significant number of animals (10%) was negative for the p27 antigen and FeLV-positive by PCR. These cats had a mean provirus load 300-fold lower than that of animals testing positive for the p27 antigen. In conclusion, an association between the provirus load and the outcome of FeLV infection was found. Detection of provirus carriers should contribute to further the control of FeLV. In addition, quantification of provirus loads will lead to a better understanding of FeLV pathogenesis and anti-retrovirus protective mechanisms.

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2001-07-01
2024-03-28
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References

  1. Berry B. T., Ghosh A. K., Kumar D. V., Spodick D. A., Roy-Burman P. 1988; Structure and function of endogenous feline leukemia virus long terminal repeats and adjoining regions. Journal of Virology 62:3631–3641
    [Google Scholar]
  2. Elder J. H., Mullins J. I. 1983; Nucleotide sequence of the envelope gene of Gardner–Arnstein feline leukemia virus B reveals unique sequence homologies with a murine mink cell focus-forming virus. Journal of Virology 46:871–880
    [Google Scholar]
  3. Essex M. E. 1982; Feline leukemia: a naturally occurring cancer of infectious origin. Epidemiologic Reviews 4:189–203
    [Google Scholar]
  4. Flynn J. N., Hanlon L., Jarrett O. 2000; Feline leukaemia virus: protective immunity is mediated by virus-specific cytotoxic T lymphocytes. Immunology 101:120–125
    [Google Scholar]
  5. Gibson U. E. M., Heid C. A., Williams P. M. 1996; A novel method for real-time quantitative RT–PCR. Genome Research 6:995–1001
    [Google Scholar]
  6. Gut M., Leutenegger C., Huder J., Pedersen N., Lutz H. 1999; One-tube fluorogenic reverse transcription–polymerase chain reaction for the quantitation of feline coronavirus. Journal of Virological Methods 77:37–46
    [Google Scholar]
  7. Hardy W. D. Jr, McClelland A. J. 1977; Feline leukemia virus. Its related diseases and control. Veterinary Clinics of North America 7:93–103
    [Google Scholar]
  8. Heid C. A., Stevens J., Livak K. J., Williams P. M. 1996; Real–time quantitative PCR. Genome Research 6:986–994
    [Google Scholar]
  9. Holland P. M., Abramson R. D., Watson R., Gelfand D. H. 1991; Detection of specific polymerase chain reaction product by utilizing the 5′→3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proceedings of the National Academy of Sciences, USA 88:7276–7280
    [Google Scholar]
  10. Hoover E. A., Mullins J. I. 1991; Feline leukemia virus infection and diseases. Journal of the American Veterinary Medical Association 199:1287–1297
    [Google Scholar]
  11. Hoover E. A., Olsen R. G., Hardy W. D. Jr, Schaller J. P., Mathes L. E., Cockerell G. L. 1975; Biologic and immunologic response of cats to experimental infection with feline leukemia virus. Bibliotheca Haematologica 43:180–183
    [Google Scholar]
  12. Hoover E. A., Mullins J. I., Quackenbush S. L., Gasper P. W. 1987; Experimental transmission and pathogenesis of immunodeficiency syndrome in cats. Blood 70:1880–1892
    [Google Scholar]
  13. Jackson M. L., Haines D. M., Meric S. M., Misra V. 1993; Feline leukemia virus detection by immunohistochemistry and polymerase chain reaction in formalin-fixed, paraffin-embedded tumor tissue from cats with lymphosarcoma. Canadian Journal of Veterinary Research 57:269–276
    [Google Scholar]
  14. Jackson M. L., Haines D. M., Taylor S. M., Misra V. 1996; Feline leukemia virus detection by ELISA and PCR in peripheral blood from 68 cats with high, moderate, or low suspicion of having FeLV-related disease. Journal of Veterinary Diagnostic Investigation 8:25–30
    [Google Scholar]
  15. Jarrett W. F. 1975; Cat leukemia and its viruses. Advances in Veterinary Science and Comparative Medicine 19:165–193
    [Google Scholar]
  16. Jarrett O., Ganiere J. P. 1996; Comparative studies of the efficacy of a recombinant feline leukemia virus vaccine. Veterinary Record 138:7–11
    [Google Scholar]
  17. Jarrett O., Hardy W. D. Jr, Golder M. C., Hay D. 1978; The frequency of occurrence of feline leukaemia virus subgroups in cats. International Journal of Cancer 21:334–337
    [Google Scholar]
  18. Klenerman P., Hengartner H., Zinkernagel R. M. 1997; A non-retroviral RNA virus persists in DNA form. Nature 390:298–301
    [Google Scholar]
  19. Kumar D. V., Berry B. T., Roy-Burman P. 1989; Nucleotide sequence and distinctive characteristics of the env gene of endogenous feline leukemia provirus. Journal of Virology 63:2379–2384
    [Google Scholar]
  20. Lee L. G., Connell C. R., Bloch W. 1993; Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Research 21:761–766
    [Google Scholar]
  21. Livak K. J., Flood S. J. A., Marmaro J., Giusti W., Deetz K. 1995; Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods and Applications 4:357–362
    [Google Scholar]
  22. Lutz H., Pedersen N., Higgins J., Hubscher U., Troy F. A., Theilen G. H. 1980; Humoral immune reactivity to feline leukemia virus and associated antigens in cats naturally infected with feline leukemia virus. Cancer Research 40:3642–3651
    [Google Scholar]
  23. Lutz H., Pedersen N. C., Durbin R., Theilen G. H. 1983; Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27. Journal of Immunological Methods 56:209–220
    [Google Scholar]
  24. Lutz H., Arnold P., Hubscher U., Egberink H., Pedersen N., Horzinek M. C. 1988; Specificity assessment of feline T-lymphotropic lentivirus serology. Zentralblatt der Veterinärmedizin Reihe B 35:773–778
    [Google Scholar]
  25. Lyamichev V., Brow M. A. D., Dahlberg J. E. 1993; Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases. Science 260:778–783
    [Google Scholar]
  26. McClelland A. J., Hardy W. D., Zuckerman E. E. 1980; Prognosis of healthy feline leukemia virus infected cats. In Development in Cancer Research pp 121–126 Edited by Hardy W. D., Essex M., McClelland A. J. Amsterdam: Elsevier;
    [Google Scholar]
  27. Miyazawa T., Jarrett O. 1997; Feline leukaemia virus proviral DNA detected by polymerase chain reaction in antigenemic but non-viraemic (‘discordant’) cats. Archives of Virology 142:323–332
    [Google Scholar]
  28. Mullins J. I., Chen C. S., Hoover E. A. 1986; Disease-specific and tissue-specific production of unintegrated feline leukaemia virus variant DNA in feline AIDS. Nature 319:333–336
    [Google Scholar]
  29. Mullins J. I., Hoover E. A., Quackenbush S. L. 1991; Disease progression and viral genome variants in experimental feline leukemia virus-induced immunodeficiency syndrome. Journal of Acquired Immune Deficiency Syndromes 4:547–557
    [Google Scholar]
  30. Quackenbush S. L., Dean G. A., Mullins J. I., Hoover E. A. 1996a; Analysis of FeLV-FAIDS provirus burden and productive infection in lymphocyte subsets in vivo . Virology 223:1–9
    [Google Scholar]
  31. Quackenbush S. L., Mullins J. I., Hoover E. A. 1996b; Replication kinetics and cell tropism of an immunosuppressive feline leukaemia virus. Journal of General Virology 77:1411–1420
    [Google Scholar]
  32. Riedel N., Hoover E. A., Gasper P. W., Nicolson M. O., Mullins J. I. 1986; Molecular analysis and pathogenesis of the feline aplastic anemia retrovirus, feline leukemia virus C-Sarma. Journal of Virology 60:242–250
    [Google Scholar]
  33. Riedel N., Hoover E. A., Dornsife R. E., Mullins J. I. 1988; Pathogenic and host range determinants of the feline aplastic anemia retrovirus. Proceedings of the National Academy of Sciences, USA 85:2758–2762
    [Google Scholar]
  34. Rohn J. L., Overbaugh J. 1995; In vivo selection of long terminal repeat alterations in feline leukemia virus-induced thymic lymphomas. Virology 206:661–665
    [Google Scholar]
  35. Rojko J. L., Kociba G. J. 1991; Pathogenesis of infection by the feline leukemia virus. Journal of the American Veterinary Medical Association 199:1305–1310
    [Google Scholar]
  36. Sarma P. S., Log T. 1973; Subgroup classification of feline leukemia and sarcoma viruses by viral interference and neutralization tests. Virology 54:160–169
    [Google Scholar]
  37. Sparkes A. H. 1997; Feline leukaemia virus: a review of immunity and vaccination. Journal of Small Animal Practice 38:187–194
    [Google Scholar]
  38. Stewart M. A., Warnock M., Wheeler A., Wilkie N., Mullins J. I., Onions D. E., Neil J. C. 1986; Nucleotide sequences of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B viruses. Journal of Virology 58:825–834
    [Google Scholar]
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