1887

Abstract

A sequence of 1425 nt was established that included the complete coat protein (CP) gene of (LBVV). The LBVV CP gene encodes a 397 amino acid protein with a predicted of 44486. Antisera raised against synthetic peptides corresponding to N-terminal or C-terminal parts of the LBVV CP reacted in Western blot analysis with a protein with an of about 48000. RNA extracted from purified particles of LBVV by using proteinase K, SDS and phenol migrated in gels as two single-stranded RNA species of approximately 7·3 kb (ss-1) and 6·6 kb (ss-2). After denaturation by heat and annealing at room temperature, the RNA migrated as four species, ss-1, ss-2 and two additional double-stranded RNAs (ds-1 and ds-2). The Northern blot hybridization analysis using riboprobes from a full-length clone of the LBVV CP gene indicated that ss-2 has a negative-sense nature and contains the LBVV CP gene. Moreover, ds-2 is a double-stranded form of ss-2. Database searches showed that the LBVV CP most resembled the nucleocapsid proteins of rhabdoviruses. These results indicate that it would be appropriate to classify LBVV as a negative-sense single-stranded RNA virus rather than as a double-stranded RNA virus.

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2001-06-01
2019-10-22
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References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25, 3389-3402.[CrossRef]
    [Google Scholar]
  2. Barany, G. & Merrifield, R. B. ( 1980; ). Solid-phase peptide synthesis In The Peptides, vol. 2 , pp. 1-284. Edited by E. Gross & J. Meienhofer. New York:Academic Press.
  3. Campbell, R. N. ( 1996; ). Fungal transmission of plant viruses. Annual Review of Phytopathology 34, 87-108.[CrossRef]
    [Google Scholar]
  4. Falk, B. W. & Tsai, J. H. ( 1998; ). Biology and molecular biology of viruses in the genus Tenuivirus. Annual Review of Phytopathology 36, 139-163.[CrossRef]
    [Google Scholar]
  5. Fazeli, C. F., Habili, N. & Rezaian, M. A. ( 1998; ). Efficient cloning of cDNA from grapevine leafroll-associated virus 4 and demonstration of probe specificity by the viral antibody. Journal of Virological Methods 70, 201-211.[CrossRef]
    [Google Scholar]
  6. Gilmore, R. D.Jr & Leong, J. C. ( 1988; ). The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus. Virology 167, 644-648.
    [Google Scholar]
  7. Habili, N., Fazeli, C. F., Ewart, A., Hamilton, R., Cirami, R., Saldarelli, P., Minafra, A. & Rezaian, M. A. ( 1995; ). Natural spread and molecular analysis of grapevine leafroll-associated virus 3 in Australia. Phytopathology 85, 1418-1422.[CrossRef]
    [Google Scholar]
  8. Hirano, H., Komatsu, S., Nakamura, A., Kikuchi, F., Kajiwara, H., Tsunasawa, S. & Sakiyama, F. ( 1991; ). Structural homology between semidwarfism-related proteins and glutelin seed protein in rice (Oryza sativa L.). Theoretical and Applied Genetics 83, 153-158.
    [Google Scholar]
  9. Huijberts, N., Blystad, D.-R. & Bos, L. ( 1990; ). Lettuce big-vein virus: mechanical transmission and relationships to tobacco stunt virus. Annals of Applied Biology 116, 463-475.[CrossRef]
    [Google Scholar]
  10. Jelkmann, W., Martin, R. R. & Maiss, E. ( 1989; ). Cloning of four plant viruses from small quantities of double-stranded RNA. Phytopathology 79, 1250-1253.[CrossRef]
    [Google Scholar]
  11. Kuwata, S. ( 1996; ). Lettuce big-vein varicosavirus In Viruses of Plants , pp. 715-717. Edited by A. A. Brunt, K. Crabtree, M. J. Dallwitz, A. J. Gibbs & L. Watson. Wallingford:CAB International.
  12. Kuwata, S. & Kubo, S. (1986). Tobacco stunt virus. AAB Descriptions of Plant Viruses, no. 313.
  13. Kuwata, S., Kubo, S., Yamashita, S. & Doi, Y. ( 1983; ). Rod-shaped particles, a probable entity of lettuce big vein virus. Annals of the Phytopathological Society of Japan 49, 246-251.[CrossRef]
    [Google Scholar]
  14. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.[CrossRef]
    [Google Scholar]
  15. Lütcke, H. A., Chow, K. C., Mickel, F. S., Moss, K. A., Kern, H. F. & Scheele, G. A. ( 1987; ). Selection of AUG initiation codons differs in plants and animals. EMBO Journal 6, 43-48.
    [Google Scholar]
  16. Mayo, M. A. ( 2000; ). Genus Varicosavirus In Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses , pp. 521-523. Edited by M. H. V. van Regenmortel, C. M. Fauquet, D. H. L. Bishop, E. B. Carstens, M. K. Estes, S. M. Lemon, J. Maniloff, M. A. Mayo, D. J. McGeoch, C. R. Pringle & R. B. Wickner. San Diego:Academic Press.
  17. Pearson, W. R. & Lipman, D. J. ( 1988; ). Improved tools for biological sequence comparison. Proceedings of the National Academy of Sciences, USA 85, 2444-2448.[CrossRef]
    [Google Scholar]
  18. Sambrook, J. & Russell, D. W. (2000). Northern hybridization. In Molecular Cloning: A Laboratory Manual, 3rd edn, pp. 7.42–7.50. Edited by J. Sambrook & D. W. Russell. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  19. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673-4680.[CrossRef]
    [Google Scholar]
  20. Uyeda, I., Kimura, I. & Shikata, E. ( 1995; ). Characterization of genome structure and establishment of vector cell lines for plant reoviruses. Advances in Virus Research 45, 249-279.
    [Google Scholar]
  21. Vetten, H. J., Lesemann, D.-E. & Dalchow, J. ( 1987; ). Electron microscopical and serological detection of virus-like particles associated with lettuce big vein disease. Journal of Phytopathology 120, 53-59.[CrossRef]
    [Google Scholar]
  22. Walsh, J. A. ( 1994; ). Effects of some biotic and abiotic factors on symptom expression of lettuce big-vein virus in lettuce (Lactuca sativa). Journal of Horticultural Science 69, 21-28.
    [Google Scholar]
  23. Zelcer, A., Weaber, K. F., Balazs, E. & Zaitlin, M. ( 1981; ). The detection and characterization of viral-related double-stranded RNAs in tobacco mosaic virus-infected plants. Virology 113, 417-427.[CrossRef]
    [Google Scholar]
  24. Zink, F. W. & Grogan, R. G. ( 1954; ). The interrelated effects of big vein and market price on the yield of head lettuce. Plant Disease Reporter 38, 844-846.
    [Google Scholar]
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