1887

Abstract

All transcripts of the human parvovirus B19 identified so far are regulated by a single promoter at map unit 6 of the viral genome, the so-called p6 promoter. This promoter is active in a wide variety of different cells. In order to identify cellular transcription factors involved in regulating promoter activity, we performed gel-retardation and supershift assays using the parts of the p6 promoter sequence shown previously to be protected in footprint experiments. Thereby, binding was demonstrated of the Oct-1 protein to an octamer motif within the p6 promoter and of the transcription factor Sp1 to three GC boxes. A specific preferential interaction of the factor Sp3 with one of these boxes was observed, indicating that the ratio Sp1:Sp3 may be involved in the regulation of promoter activity. Consensus sites for the regulatory protein YY1 are located close to the GC boxes and the octamer motif, to which this factor binds efficiently.

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2001-06-01
2020-06-01
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References

  1. Anderson M. J., Jones S. E., Fisher-Hoch S. P., Lewis E., Hall S. M., Bartlett C. L., Cohen B. J., Mortimer P. P., Pereira M. S.. 1983; Human parvovirus, the cause of erythema infectiosum (fifth disease)?. Lancet i, 1378
    [Google Scholar]
  2. Blundell M. C., Astell C. R.. 1989; A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription. Journal of Virology63:4814–4823
    [Google Scholar]
  3. Blundell M. C., Beard C., Astell C. R.. 1987; In vitro identification of a B19 parvovirus promoter. Virology157:534–538
    [Google Scholar]
  4. Brown K. A.. 1984; Another viral candidate. Nature309:582
    [Google Scholar]
  5. Deiss V., Tratschin J. D., Weitz M., Siegl G.. 1990; Cloning of the human parvovirus B19 genome and structural analysis of its palindromic termini. Virology175:247–254
    [Google Scholar]
  6. Dennig J., Hagen G., Beato M., Suske G.. 1995; Members of the Sp transcription factor family control transcription from the uteroglobin promoter. Journal of Biological Chemistry270:12737–12744
    [Google Scholar]
  7. Dignam J. D., Lebovitz R. M., Roeder R. G.. 1983; Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Research11:1475–1489
    [Google Scholar]
  8. Doerig C., Hirt B., Antonietti J. P., Beard P.. 1990; Nonstructural protein of parvoviruses B19 and minute virus of mice controls transcription. Journal of Virology64:387–396
    [Google Scholar]
  9. Gareus R., Gigler A., Hemauer A., Leruez-Ville M., Morinet F., Wolf H., Modrow S.. 1998; Characterization of cis-acting and NS1 protein-responsive elements in the p6 promoter of parvovirus B19. Journal of Virology72:609–616
    [Google Scholar]
  10. Gigler A., Dorsch S., Hemauer A., Williams C., Kim S., Young N. S., Zolla-Pazner S., Wolf H., Gorny M. K., Modrow S.. 1999; Generation of neutralizing human monoclonal antibodies against parvovirus B19 proteins. Journal of Virology73:1974–1979
    [Google Scholar]
  11. Hagen G., Müller S., Beato M., Suske G.. 1992; Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes. Nucleic Acids Research20:5519–5525
    [Google Scholar]
  12. Hagen G., Muller S., Beato M., Suske G.. 1994; Sp1-mediated transcriptional activation is repressed by Sp3. EMBO Journal13:3843–3851
    [Google Scholar]
  13. Hata Y., Duh E., Zhang K., Robinson G. S., Aiello L. P.. 1998; Transcription factors Sp1 and Sp3 alter vascular endothelial growth factor receptor expression through a novel recognition sequence. Journal of Biological Chemistry273:19294–19303
    [Google Scholar]
  14. Kalenik J. L., Chen D., Bradley M. E., Chen S. J., Lee T. C.. 1997; Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1. Nucleic Acids Research25:843–849
    [Google Scholar]
  15. Kennett S. B., Udvadia A. J., Horowitz J. M.. 1997; Sp3 encodes multiple proteins that differ in their capacity to stimulate or repress transcription. Nucleic Acids Research25:3110–3117
    [Google Scholar]
  16. Klug J., Beato M.. 1996; Binding of YY1 to a site overlapping a weak TATA box is essential for transcription from the uteroglobin promoter in endometrial cells. Molecular and Cellular Biology16:6398–6407
    [Google Scholar]
  17. Kwon H. S., Kim M. S., Edenberg H. J., Hur M. W.. 1999; Sp3 and Sp4 can repress transcription by competing with Sp1 for the core cis-elements on the human ADH5/FDH minimal promoter. Journal of Biological Chemistry274:20–28
    [Google Scholar]
  18. Liu J. M., Green S. W., Hao Y. S., McDonagh K. T., Young N. S., Shimada T.. 1991; Upstream sequences within the terminal hairpin positively regulate the P6 promoter of B19 parvovirus. Virology185:39–47
    [Google Scholar]
  19. Luo W., Astell C. R.. 1993; A novel protein encoded by small RNAs of parvovirus B19. Virology195:448–455
    [Google Scholar]
  20. Momoeda M., Kawase M., Jane S. M., Miyamura K., Young N. S., Kajigaya S.. 1994; The transcriptional regulator YY1 binds to the 5′-terminal region of B19 parvovirus and regulates p6 promoter activity. Journal of Virology68:7159–7168
    [Google Scholar]
  21. Nielsen S. J., Præstegaard M., Jørgensen H. F., Clark B. F. C.. 1998; Different Sp1 family members differentially affect transcription from the human elongation factor 1 A-1 gene promoter. Biochemical Journal333:511–517
    [Google Scholar]
  22. Ozawa K., Ayub J., Hao Y. S., Kurtzman G., Shimada T., Young N.. 1987; Novel transcription map for the B19 (human) pathogenic parvovirus. Journal of Virology61:2395–2406
    [Google Scholar]
  23. Pattison J. R., Jones S. E., Hodgson J., Davis L. R., White J. M., Stroud C. E., Murtaza L.. 1981; Parvovirus infections and hypoplastic crisis in sickle-cell anaemia. Lanceti:664–665
    [Google Scholar]
  24. Qin H., Sun Y., Benveniste E. N.. 1999; The transcription factors Sp1, Sp3, and AP-2 are required for constitutive matrix metalloproteinase-2 gene expression in astroglioma cells. Journal of Biological Chemistry274:29130–29137
    [Google Scholar]
  25. Reid D. M., Reid T. M., Brown T., Rennie J. A., Eastmond C. J.. 1985; Human parvovirus-associated arthritis: a clinical and laboratory description. Lanceti:422–425
    [Google Scholar]
  26. Tsai C. N., Lee C. M., Chien C. K., Kuo S. C., Chang Y. S.. 1999; Additive effect of Sp1 and Sp3 in regulation of the ED-L1E promoter of the EBV LMP 1 gene in human epithelial cells. Virology261:288–294
    [Google Scholar]
  27. Vassias I., Hazan U., Michel Y., Sawa C., Handa H., Gouya L., Morinet F.. 1998; Regulation of human B19 parvovirus promoter expression by hGABP (E4TF1) transcription factor. Journal of Biological Chemistry273:8287–8293
    [Google Scholar]
  28. Wildhage I., Trusheim H., Goke B., Lankat-Buttgereit B.. 1999; Gene expression of the human glucagon-like peptide-1 receptor is regulated by Sp1 and Sp3. Endocrinology140:624–631
    [Google Scholar]
  29. Wingender E., Chen X., Hehl R., Karas H., Liebich I., Matys V., Meinhardt T., Pruß M., Reuter I., Schacherer F.. 2000; TRANSFAC: an integrated system for gene expression regulation. Nucleic Acids Research28:316–319
    [Google Scholar]
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